Change regularity of leaf color of tea plant P113 (Camellia tachangensis F. C. Zhang) by metabolomics

doi:10.3969/j.issn.1004-1524.2022.10.06

Acta Agriculturae Zhejiangensis ›› 2022, Vol. 34 ›› Issue (10): 2121-2131.DOI: 10.3969/j.issn.1004-1524.2022.10.06

• Crop Science • Previous Articles Next Articles

Change regularity of leaf color of tea plant P113 (Camellia tachangensis F. C. Zhang) by metabolomics

LI Fang¹(), LIU Xia¹, HUANG Zheng², HE Yingqin¹, SONG Qinfei¹, NIU Suzhen^*()

1. College of Tea Science, Guizhou University, Guiyang 550025, China
2. College of Economics and Management, Guiyang Institute of Humanities and Science, Guiyang 550025, China

Received:2021-11-18 Online:2022-10-25 Published:2022-10-26
Contact: NIU Suzhen

Abstract

Abstract:

To explore the change regularity of leaf color of tea plant P113 with the seasons, and lay a research foundation for the identification and development of special tea germplasm resources, tea plant P113(Camellia tachangensis F. C. Zhang) was used as material, and multiple reaction monitoring (MRM) was used to identify the flavonoids metabolites of one bud with two leaves of P113 in spring, summer and autumn. The results showed that 165 ion peaks of flavonoids were detected, including 71 positive ion peaks and 94 negative ion peaks, including anthocyanins (6.06%), biflavones (2.42%), chalcone (0.6%), dihydroflavones (4.24%), dihydroflavonols (3.03%), flavanols (9.70%), flavonoids (26.67%), flavonoid carbonosides (4.24%), flavonols (26.67%) and isoflavones (2.42%), proanthocyanidins (7.88%) and tannins (6.06%). There were 12 kinds of flavonoids in total. Principal component analysis showed that the variation of metabolite contents between spring, summer and autumn were large, and the variation of metabolite content within the season were small, indicating that flavonoids had undergone significant dynamic changes in the process of seasonal change. Venn analysis showed that there were 158 common metabolites in spring and summer, 160 common metabolites in summer and autumn, 157 common metabolites in spring and autumn, and 2 specific metabolites (isoorange flavone and 3,5,6,7,8,3', 4'-heptamethoxyflavone) existed in spring, and there were no specific metabolites in summer and autumn. In general, most flavonoids metabolites were expressed in each period. Correlation analysis showed that anthocyanins were positively correlated with dihydroflavonols, flavonoids, flavone carboglycosides, flavonols and procyanidins, and negatively correlated with biflavones, chalcone, dihydroflavonoids, flavanols and tannins. Therefore, this study suggested that the leaf color purple change of C.tachangensis P113 was closely related to the content of flavonoids, especially anthocyanins.

Key words: metabolome, Camellia tachangensis F.C.Zhang, flavonoids, leaf color

CLC Number:

S571.1

LI Fang, LIU Xia, HUANG Zheng, HE Yingqin, SONG Qinfei, NIU Suzhen. Change regularity of leaf color of tea plant P113 (Camellia tachangensis F. C. Zhang) by metabolomics[J]. Acta Agriculturae Zhejiangensis, 2022, 34(10): 2121-2131.

Figures/Tables 8

Fig.1 Growth of P113 tea plant in spring (A), summer (B) and autumn (C)

Fig.2 Superposition of total ion current (TIC) of QC samples detected by mass spectrometry

Fig.3 Score of principal component analysis A, B and C represented samples in spring, summer and autumn, respectively. The greater the proportion of principal component, the greater the reliability of the sub sample representing the principal component.

Fig.4 Proportion of metabolite proportion

Fig.5 Venn diagram between samples in different seasons A, B and C represented spring, summer and autumn. Circles of different colors represented the collection of metabolites in different seasons, and overlapping circles represented the same metabolites in different seasons, and the number indicated the number of metabolites.

Fig.6 Expression of flavonoid in different seasons The ordinate of all histograms in figure A represented the normalized value of the peak (Fold change), and the abscissa A, B and C represented spring, summer and autumn, respectively; In figure B, the ordinate represented the metabolite name, the abscissa represented the peak normalized value, and different colors represented different seasons.

Fig.7 Heat map of correlation between different categories of metabolites The right and lower sides were metabolite category names, the left and upper sides were sample clustering trees, and the legend in the upper left corner was the color range of different r values. The redder the color, the greater the correlation between metabolites, and the bluer the color, the smaller the correlation between metabolites.

Fig.8 Metabolic pathway of flavonoids

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Change regularity of leaf color of tea plant P113 (Camellia tachangensis F. C. Zhang) by metabolomics

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