Abstract:

Color is one of the most important traits of ornamental plants and also an important direction of ornamental horticulture breeding. To investigate the presentation mechanism of red-blue color of Ananas comosus var. bracteatus, the primary analysis of the kinds and concentration of anthocyanins in different tissues had been made, the promoter of AbF3'5'H was used to build the bait vector, and the yeast one-hybrid (Y1H) library was used to select the transcription factors that control the gene’s upstream activity; The yeast one-hybrid technique was used to verify the interaction between the selected transcription factors and the AbF3'5'H promoter. The results showed that the phenotypic color of the tissues were determined by the kinds and relative proportion of anthocyanins. AbF3'5'H was a key gene functioned in the species branch determination of the anthocyanin synthesis pathway, and was crucial for regulating the kinds and relative proportion of anthocyanins; AbBTB/POZ, AbHSP81-1 and AbGLOX-like could bind to the promoter of AbF3'5'H to regulate its transcription, suggesting that they might play important role in the regulation of anthocyanin anabolism in A. comosus var. bracteatus. These results had important theoretical and practical significance in understanding the regulation mechanism of anthocyanin biosynthesis, investigating the color formation mechanism and cultivating new leaf color variants of A. comosus var. bracteatus.

Key words: Ananas comosus var. bracteatus, F3'5'H, yeast one-hybrid, regulatory factor