OSA-modified porous starch loading and improving bioavailability of naringin

doi:10.3969/j.issn.1004-1524.2023.02.20

Acta Agriculturae Zhejiangensis ›› 2023, Vol. 35 ›› Issue (2): 425-433.DOI: 10.3969/j.issn.1004-1524.2023.02.20

• Food Science • Previous Articles Next Articles

OSA-modified porous starch loading and improving bioavailability of naringin

WANG Lu¹(), DING Zhe¹^,², LU Shengmin¹^,^*(), JIANG Hao¹^,³, ZHENG Meiyu¹, YANG Ying¹

1. Institute of Food Science, Zhejiang Academy of Agricultural Sciences, Hangzhou 310021, China
2. College of Food Science and Engineering, Zhejiang University of Technology, Hangzhou 310014, China
3. School of Chemical Sciences, Auckland University, Auckland 1010, New Zealand

Received:2022-04-20 Online:2023-02-25 Published:2023-03-14
Contact: LU Shengmin

Abstract

Abstract:

In order to enhance the loading of naringin (NA) in OSA modified porous starch (OSAPS) by optimization experiments and further enhance the in vivo bioavailability of NA with the form of complex, OSAPS-NA complexes were prepared by antisolvent precipitation method and metabolic kinetic studies were performed on the complexes prepared under optimal conditions. The oral bioavailability of the raw NA material and the OSAPS-NA complex was evaluated by fitting the compartmental model and the statistical moment model. The result showed that when acetone was applied as the solvent, solvent co-precipitation combined with ultrasonication significantly enhanced the loading rate of NA in OSAPS, and the highest loading rate of NA in OSAPS was achieved when the volume ratio of antisolvent (n-hexane) was 0.6, the sonication time was 200 W and the sonication time was 20 min. The oral relative bioavailability results indicated that the area under the drug-time curve of NA in the OSAPS-NA complex prepared under optimal conditions was 9.80 times that of the original NA material, and the time to peak was reduced from 60 min to 15 min. The study successfully optimized the OSAPS-NA complex, resulting in approximately (71.49±2.26)% loading of NA. And by being loaded by OSAPS, the relative oral bioavailability of NA was increased by 9.80 times.

Key words: OSA modified starch, porous starch, naringin, antisolvent precipitation method, oral bioavailability

CLC Number:

WANG Lu, DING Zhe, LU Shengmin, JIANG Hao, ZHENG Meiyu, YANG Ying. OSA-modified porous starch loading and improving bioavailability of naringin[J]. Acta Agriculturae Zhejiangensis, 2023, 35(2): 425-433.

Figures/Tables 9

References 22

[1]	BAJAJ R, SINGH N, KAUR A. Properties of octenyl succinic anhydride (OSA) modified starches and their application in low fat mayonnaise[J]. International Journal of Biological Macromolecules, 2019, 131: 147-157.
[2]	于洋. 辛烯基琥珀酸淀粉酯的合成[D]. 石家庄: 河北科技大学, 2009.
	YU Y. The synthesis of octenylsuccinate starch acetate[D]. Shijiazhuang: Hebei University of Science and Technology, 2009. (in Chinese with English abstract)
[3]	马铁铮, 邓秀琴, 王静. 制备食品微胶囊壁材用改性多糖的应用进展[J]. 中国食品学报, 2018, 18(6): 212-220.
	MA T Z, DENG X Q, WANG J. Progress on application of modified polysaccharides for wall materials of food microcapsules[J]. Journal of Chinese Institute of Food Science and Technology, 2018, 18(6): 212-220. (in Chinese with English abstract)
[4]	刘坤. 淀粉基自组装胶束递送系统的构建及其M细胞靶向特性研究[D]. 广州: 华南理工大学, 2018.
	LIU K. Fabrication of starch-based self-assembled micelles delivery system and their M-cell targeting property[D]. Guangzhou: South China University of Technology, 2018. (in Chinese with English abstract)
[5]	靳蔷薇. 天然高分子乳化剂的比较研究:玉米纤维胶及改性淀粉、阿拉伯胶和大豆多糖的流变学和乳化稳定机理[D]. 上海: 上海交通大学, 2017.
	JIN Q W. A comparison study of natural polysaccharide emulsifiers: rheological response and emulsion stabilization mechanisms of corn fiber gum, modified starch, gum arabic and soybean soluble polysaccharide[D]. Shanghai: Shanghai Jiao Tong University, 2017. (in Chinese with English abstract)
[6]	曹亚飞. 疏水多孔淀粉的制备及其吸油性能研究[D]. 广州: 华南理工大学, 2016.
	CAO Y F. Preparation of hydrophobic porous starch and application in oil absorbency[D]. Guangzhou: South China University of Technology, 2016. (in Chinese with English abstract)
[7]	CHEN J H, WANG Y X, LIU J, et al. Preparation, characterization, physicochemical property and potential application of porous starch: a review[J]. International Journal of Biological Macromolecules, 2020, 148: 1169-1181.
[8]	SUO F Y, LIU X, LI C S, et al. Mesoporous activated carbon from starch for superior rapid pesticides removal[J]. International Journal of Biological Macromolecules, 2019, 121: 806-813.
[9]	LI C Y, LOU T, YAN X, et al. Fabrication of pure chitosan nanofibrous membranes as effective absorbent for dye removal[J]. International Journal of Biological Macromolecules, 2018, 106: 768-774.
[10]	BELINGHERI C, FERRILLO A, VITTADINI E. Porous starch for flavor delivery in a tomato-based food application[J]. LWT-Food Science and Technology, 2015, 60(1): 593-597.
[11]	ZHU J Z, ZHONG L, CHEN W X, et al. Preparation and characterization of pectin/chitosan beads containing porous starch embedded with doxorubicin hydrochloride: a novel and simple colon targeted drug delivery system[J]. Food Hydrocolloids, 2019, 95: 562-570.
[12]	高彦祥, 方政. 柑橘类果汁加工副产品综合利用研究进展[J]. 饮料工业, 2005, 8(1): 1-7.
	GAO Y X, FANG Z. Progress in research on comprehensive utilization of by-products of citrus juice processing[J]. The Beverage Industry, 2005, 8(1): 1-7. (in Chinese with English abstract)
[13]	向露, 唐伟敏, 郑美瑜, 等. 不同分子质量辛烯基琥珀酸蜡质玉米淀粉酯对柚皮素的增溶作用研究[J]. 食品科技, 2020, 45(4): 226-231.
	XIANG L, TANG W M, ZHENG M Y, et al. Solubilization of naringenin by octenyl succinic acid esterified waxy corn starch with different molecular weights[J]. Food Science and Technology, 2020, 45(4): 226-231. (in Chinese with English abstract)
[14]	李海燕, 马云翔, 俞力月, 等. 辛烯基琥珀酸酐改性多孔淀粉结构表征[J]. 中国粮油学报, 2020, 35(6): 70-75.
	LI H Y, MA Y X, YU L Y, et al. Structure characterization of octenyl succinic anhydride modified porous starch[J]. Journal of the Chinese Cereals and Oils Association, 2020, 35(6): 70-75. (in Chinese with English abstract)
[15]	李媛媛. 苹果树枝中根皮素的提取纯化、载药体系构建与评价[D]. 哈尔滨: 东北林业大学, 2019.
	LI Y Y. Separation and purification of phloretin from apple tree branches and design and evaluation on its drug delivery system[D]. Harbin: Northeast Forestry University, 2019. (in Chinese with English abstract)
[16]	WANG L, LU S M, DENG Y P, et al. Pickering emulsions stabilized by luteolin micro-nano particles to improve the oxidative stability of pine nut oil[J]. Journal of the Science of Food and Agriculture, 2021, 101(4): 1314-1322.
[17]	LI Z, WEI C X. Morphology, structure, properties and applications of starch ghost: a review[J]. International Journal of Biological Macromolecules, 2020, 163: 2084-2096.
[18]	WANG J Q, LV X R, LAN T, et al. Modification in structural, physicochemical, functional, and in vitro digestive properties of kiwi starch by high-power ultrasound treatment[J]. Ultrasonics Sonochemistry, 2022, 86: 106004.
[19]	LIN Q Q, LIANG R, ZHONG F, et al. Self-assembled micelles based on OSA-modified starches for enhancing solubility of β-carotene: effect of starch macromolecular architecture[J]. Journal of Agricultural and Food Chemistry, 2019, 67(23): 6614-6624.
[20]	唐圆, 谭周进, 谢果珍. 植物中苷类化合物的水解及其药理作用研究进展[J]. 现代农业科技, 2021(17): 216-219.
	TANG Y, TAN Z J, XIE G Z. Research progress on hydrolysis and pharmacological action of glycosides in plants[J]. Modern Agricultural Science and Technology, 2021(17): 216-219. (in Chinese with English abstract)
[21]	ANAR P J, SINGH R P, VAIBHAV P, et al. Application of mathematical models in drug release kinetics of Lagerstroemia speciosa extract-phospholipid complex[J]. Research Journal of Pharmacy and Technology, 2022, 15(3): 1257-1262.
[22]	LI X, LU Y, WANG Y X, et al. Thermo-responsive injectable naringin-loaded hydrogel polymerised sodium alginate/bioglass delivery for articular cartilage[J]. Drug Delivery, 2021, 28(1): 1290-1300.

序号	V_正己烷/V_丙酮 V_n-Hexane /V_Acetone	m_OSAPS/m_NA	超声强度 Ultrasound intensity/W	超声时间 Ultrasound time/ min
1	0.1	2	80	10
2	0.2	5	120	20
3	0.3	10	160	30
4	0.4	15	200	40
5	0.5	20		50
6	0.6
7	0.7
8	0.8

序号	V_正己烷/V_丙酮 V_n-Hexane /V_Acetone	m_OSAPS/m_NA	超声强度 Ultrasound intensity/W	超声时间 Ultrasound time/ min
1	0.1	2	80	10
2	0.2	5	120	20
3	0.3	10	160	30
4	0.4	15	200	40
5	0.5	20		50
6	0.6
7	0.7
8	0.8

样品名称 Sample name	模拟胃液Simulated gastric fluid			模拟肠液Simulated intestinal fluid
样品名称 Sample name	k₁	n₁	R²	k₂	n₂	R²
NA原材料Raw NA	5.88±1.35	0.19±0.04	0.849 9	9.75±1.23	0.38±0.08	0.979 1
OSAPS-NA复合物OSAPS-NA complex	33.40±3.08	0.47±0.06	0.991 1	31.26±2.38	0.46±0.05	0.994 9

样品名称 Sample name	模拟胃液Simulated gastric fluid			模拟肠液Simulated intestinal fluid
样品名称 Sample name	k₁	n₁	R²	k₂	n₂	R²
NA原材料Raw NA	5.88±1.35	0.19±0.04	0.849 9	9.75±1.23	0.38±0.08	0.979 1
OSAPS-NA复合物OSAPS-NA complex	33.40±3.08	0.47±0.06	0.991 1	31.26±2.38	0.46±0.05	0.994 9

参数 Parameters	NA原材料 Raw NA	OSAPS-NA复合物 OSAPS-NA complex
C_max/(μg·mL^-1)	0.88±0.02	4.02±0.50^**
T_max/min	60.00±0.01	15.00±0.01^**
t_1/2α/min	34.96±2.21	69.32±2.07^*
t_1/2β/min	69.31±3.47	69.32±3.59
AUC_(0-2880)/	636.15±25.20	6 231.82±100.11^**
(μg·L^-1·min^-1)
AUC_(0-∞)/	1 553.07±177.87	27 398.23±261.10^**
(μg·L^-1·min^-1)
MRT_(0-∞)/min	5 566.07±186.95	11 129.46±239.65^**
F_rb/%	—	979.61±56.79

OSA-modified porous starch loading and improving bioavailability of naringin

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References 22

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序号	V_正己烷/V_丙酮 V_n-Hexane /V_Acetone	m_OSAPS/m_NA	超声强度 Ultrasound intensity/W	超声时间 Ultrasound time/ min
1	0.1	2	80	10
2	0.2	5	120	20
3	0.3	10	160	30
4	0.4	15	200	40
5	0.5	20		50
6	0.6
7	0.7
8	0.8

序号	V_正己烷/V_丙酮 V_n-Hexane /V_Acetone	m_OSAPS/m_NA	超声强度 Ultrasound intensity/W	超声时间 Ultrasound time/ min
1	0.1	2	80	10
2	0.2	5	120	20
3	0.3	10	160	30
4	0.4	15	200	40
5	0.5	20		50
6	0.6
7	0.7
8	0.8

序号	V_正己烷/V_丙酮 V_n-Hexane /V_Acetone	m_OSAPS/m_NA	超声强度 Ultrasound intensity/W	超声时间 Ultrasound time/ min
1	0.1	2	80	10
2	0.2	5	120	20
3	0.3	10	160	30
4	0.4	15	200	40
5	0.5	20		50
6	0.6
7	0.7
8	0.8

序号	V_正己烷/V_丙酮 V_n-Hexane /V_Acetone	m_OSAPS/m_NA	超声强度 Ultrasound intensity/W	超声时间 Ultrasound time/ min
1	0.1	2	80	10
2	0.2	5	120	20
3	0.3	10	160	30
4	0.4	15	200	40
5	0.5	20		50
6	0.6
7	0.7
8	0.8

序号	V_正己烷/V_丙酮 V_n-Hexane /V_Acetone	m_OSAPS/m_NA	超声强度 Ultrasound intensity/W	超声时间 Ultrasound time/ min
1	0.1	2	80	10
2	0.2	5	120	20
3	0.3	10	160	30
4	0.4	15	200	40
5	0.5	20		50
6	0.6
7	0.7
8	0.8

序号	V_正己烷/V_丙酮 V_n-Hexane /V_Acetone	m_OSAPS/m_NA	超声强度 Ultrasound intensity/W	超声时间 Ultrasound time/ min
1	0.1	2	80	10
2	0.2	5	120	20
3	0.3	10	160	30
4	0.4	15	200	40
5	0.5	20		50
6	0.6
7	0.7
8	0.8