Construction of high-density bin marker genetic map and QTL mapping for fruit length in Luffa spp.

doi:10.3969/j.issn.1004-1524.2023.03.12

Acta Agriculturae Zhejiangensis ›› 2023, Vol. 35 ›› Issue (3): 590-597.DOI: 10.3969/j.issn.1004-1524.2023.03.12

• Horticultural Science • Previous Articles Next Articles

Construction of high-density bin marker genetic map and QTL mapping for fruit length in Luffa spp.

CUI Junjie(), LYU Zhen, YANG Tianwen, WANG Jing, HONG Yu, CAO Yi^*()

Department of Horticulture, Foshan University, Foshan 528225, Guangdong, China

Received:2022-05-05 Online:2023-03-25 Published:2023-04-07

Abstract

Abstract:

To explore the construction of a high-density genetic map of Luffa spp. and to identify the genetic loci for fruit length variation, a BC₁ population of 67 individuals was developed by crossing two inbred lines, Luffa acutangula ‘Shengyou’ and L. cylindrica ‘Pingguo’, with significant differences in fruit length. A high-density interspecific genetic map of Luffa spp. was constructed using the bin markers based on whole genome resequencing. The map consisted of 9 299 bin markers distributed in 14 linkage groups spanning 2 956.57 cM (about 80.47% of the Luffa cylindrica genome) with an average genetic distance of 0.32 cM. Combined with the genetic map, the MQM model in MapQTL 6 software was used to detect QTL for fruit length in Luffa spp. A QTL locus qFL1.1 for fruit length in Luffa spp. was mapped between markers scaf1-bin33 (19.76 cM) and scaf1-bin359 (92.84 cM) on linkage group Scaf 1. The LOD of qFL1.1 was 4.21, the contribution rate was 28.90% and the additive effect was 6.04. The results provided a theoretical basis for genetic mining based on the interspecific genetic map in Luffa spp.

Key words: Luffa spp., bin marker, interspecific genetic map, fruit length, QTL mapping

CLC Number:

S642.4

CUI Junjie, LYU Zhen, YANG Tianwen, WANG Jing, HONG Yu, CAO Yi. Construction of high-density bin marker genetic map and QTL mapping for fruit length in Luffa spp.[J]. Acta Agriculturae Zhejiangensis, 2023, 35(3): 590-597.

Figures/Tables 6

References 30

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材料 Sample	平均值 Mean/cm	标准差 Standard deviation	标准误 Standard error of the mean	正态分布W检验(P) Normal distribution W test (P)	偏度 Skewness	峰度 Kurtosis
盛优长丝瓜Shengyou	62.70	5.06	1.69	0.20	-0.44	-0.09
苹果丝瓜Pingguo	13.63	0.76	0.25	0.98	-0.06	-1.88
F₁	41.04	2.06	0.69	0.59	-0.39	-0.95
BC₁	19.76	5.65	0.75	0.02	0.83	-0.13

材料 Sample	平均值 Mean/cm	标准差 Standard deviation	标准误 Standard error of the mean	正态分布W检验(P) Normal distribution W test (P)	偏度 Skewness	峰度 Kurtosis
盛优长丝瓜Shengyou	62.70	5.06	1.69	0.20	-0.44	-0.09
苹果丝瓜Pingguo	13.63	0.76	0.25	0.98	-0.06	-1.88
F₁	41.04	2.06	0.69	0.59	-0.39	-0.95
BC₁	19.76	5.65	0.75	0.02	0.83	-0.13

连锁群 Linkage groups	标记数量 Number of markers	连锁群长度 Linkage group length/cM	平均遗传距离 Average distance/cM	覆盖基因组大小 Genome coverage/Mb	重组率(cM/Mb) Recombination rate (cM/Mb)
Scaf 1	948	318.34	0.34	56.71	5.61
Scaf 2	1 365	310.58	0.23	51.04	6.08
Scaf 3	1 097	283.53	0.26	49.46	5.73
Scaf 4	417	98.44	0.24	48.32	2.04
Scaf 5	700	258.13	0.37	48.36	5.34
Scaf 6	509	333.61	0.66	48.61	6.86
Scaf 7	1 035	248.23	0.24	48.37	5.13
Scaf 8	771	291.90	0.38	45.43	6.43
Scaf 9	127	35.70	0.28	4.81	7.42
Scaf 10-1	130	25.98	0.20	4.62	5.62
Scaf 10-2	153	21.25	0.14	4.11	5.17
Scaf 11	729	275.49	0.38	44.99	6.12
Scaf 12	538	242.54	0.45	41.69	5.82
Scaf 13	780	212.85	0.27	41.82	5.09
总数Total	9 299	2 956.57	0.32	538.33	5.49

连锁群 Linkage groups	标记数量 Number of markers	连锁群长度 Linkage group length/cM	平均遗传距离 Average distance/cM	覆盖基因组大小 Genome coverage/Mb	重组率(cM/Mb) Recombination rate (cM/Mb)
Scaf 1	948	318.34	0.34	56.71	5.61
Scaf 2	1 365	310.58	0.23	51.04	6.08
Scaf 3	1 097	283.53	0.26	49.46	5.73
Scaf 4	417	98.44	0.24	48.32	2.04
Scaf 5	700	258.13	0.37	48.36	5.34
Scaf 6	509	333.61	0.66	48.61	6.86
Scaf 7	1 035	248.23	0.24	48.37	5.13
Scaf 8	771	291.90	0.38	45.43	6.43
Scaf 9	127	35.70	0.28	4.81	7.42
Scaf 10-1	130	25.98	0.20	4.62	5.62
Scaf 10-2	153	21.25	0.14	4.11	5.17
Scaf 11	729	275.49	0.38	44.99	6.12
Scaf 12	538	242.54	0.45	41.69	5.82
Scaf 13	780	212.85	0.27	41.82	5.09
总数Total	9 299	2 956.57	0.32	538.33	5.49

Construction of high-density bin marker genetic map and QTL mapping for fruit length in Luffa spp.

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