Acta Agriculturae Zhejiangensis ›› 2025, Vol. 37 ›› Issue (1): 203-216.DOI: 10.3969/j.issn.1004-1524.20240081
• Quality and Safety of Agricultural Products • Previous Articles Next Articles
YU Hongwei1(), DENG Huidan2, YANG Hua2, XIAO Yingping2, DING Xiangying3,*(
), JI Xiaofeng2,*(
)
Received:
2024-01-16
Online:
2025-01-25
Published:
2025-02-14
CLC Number:
YU Hongwei, DENG Huidan, YANG Hua, XIAO Yingping, DING Xiangying, JI Xiaofeng. Research progress on detection technology and analysis of veterinary drug residues in animal-derived foods[J]. Acta Agriculturae Zhejiangensis, 2025, 37(1): 203-216.
前处理方法 Pre-treatment method | 优点 Advantage | 缺点 Disadvantage | 参考文献 Reference |
---|---|---|---|
固相萃取法 Solid-phase extraction | 提取效果好Good extraction effect 选择性强Strong selectivity 分离时间较短Short separation time | 固相萃取柱成本高High cost 回收误差大Large recovery error | [ |
液液萃取法 Liquid-liquid extraction | 操作方法简单Simple operation method 分离效果好Good separation effect 适用性强Strong applicability | 有机液体使用量大Large organic liquids 萃取溶剂污染环境 Extraction solvents pollute the environment | [ |
QuEChERs萃取 QuEChERs extraction | 准确度高High accuracy 适用范围广Wide scope of application 有机液体使用量少Less organic liquid | 净化效果不足Insufficient purification effect 易受到基质干扰Susceptible to matrix interference | [ |
加速溶剂萃取 Accelerated solvent extraction | 安全快速Safe and fast 萃取效率高High extraction efficiency 基质效应小Low matrix effect 适合大批量样品检测 Suitable for large-batch sample testing | 需要高温条件High temperature conditions required 需要高压条件High pressure conditions required 仪器操作复杂Complex instrument operation | [ |
新型微萃取 New micro extraction | 富集痕量分析物 Enrichment of trace analytes 高通量检测High-throughput detection 易实现自动化Easy automation | 复杂基质提取效率低 Efficient complex matrix extraction 耗时长Time-consuming | [ |
超临界流体萃取 Super-critical extraction | 操作简单Simple operation 无污染Non-pollution | 设备成本高 High equipment cost | [ |
微波辅助萃取 Microwave-assisted extraction | 经济环保 Economic and environmental protection 提取效率高High extraction efficiency 测量准确度高High measurement accuracy | 选择性低 Low selectivity | [ |
Table 1 Comparison of the advantages and disadvantages of pretreatment methods for detection of veterinary drug residues in animal-derived foods
前处理方法 Pre-treatment method | 优点 Advantage | 缺点 Disadvantage | 参考文献 Reference |
---|---|---|---|
固相萃取法 Solid-phase extraction | 提取效果好Good extraction effect 选择性强Strong selectivity 分离时间较短Short separation time | 固相萃取柱成本高High cost 回收误差大Large recovery error | [ |
液液萃取法 Liquid-liquid extraction | 操作方法简单Simple operation method 分离效果好Good separation effect 适用性强Strong applicability | 有机液体使用量大Large organic liquids 萃取溶剂污染环境 Extraction solvents pollute the environment | [ |
QuEChERs萃取 QuEChERs extraction | 准确度高High accuracy 适用范围广Wide scope of application 有机液体使用量少Less organic liquid | 净化效果不足Insufficient purification effect 易受到基质干扰Susceptible to matrix interference | [ |
加速溶剂萃取 Accelerated solvent extraction | 安全快速Safe and fast 萃取效率高High extraction efficiency 基质效应小Low matrix effect 适合大批量样品检测 Suitable for large-batch sample testing | 需要高温条件High temperature conditions required 需要高压条件High pressure conditions required 仪器操作复杂Complex instrument operation | [ |
新型微萃取 New micro extraction | 富集痕量分析物 Enrichment of trace analytes 高通量检测High-throughput detection 易实现自动化Easy automation | 复杂基质提取效率低 Efficient complex matrix extraction 耗时长Time-consuming | [ |
超临界流体萃取 Super-critical extraction | 操作简单Simple operation 无污染Non-pollution | 设备成本高 High equipment cost | [ |
微波辅助萃取 Microwave-assisted extraction | 经济环保 Economic and environmental protection 提取效率高High extraction efficiency 测量准确度高High measurement accuracy | 选择性低 Low selectivity | [ |
检测技术 Detection technique | 优点 Advantage | 缺点 Disadvantage | 参考文献 Reference |
---|---|---|---|
液相色谱法 Liquid chromatography | 分离效率高High separation efficiency 选择性好Good selectivity 检测灵敏度高High detection sensitivity 适用范围广Wide range of application | 设备维护成本昂贵 The equipment is expensive to maintain 检测成本高High detection cost 分析时间长Long analysis time | [ |
气相色谱-质谱法 Gas chromatography- mass spectrometry | 检测速度快Fast detection speed 精准度高High accuracy 成本低Low cost | 主要适用于可气化或衍生的小分子药物 Small molecule drugs are mainly suitable for the gasified or derived drugs 检测后信号输出须矫正 The signal output should be corrected after detection | [ |
液相色谱-串联质谱法 Liquid chromatography- mass spectrometry | 适用范围广Wide scope of application 分离能力强Strong separation ability 数据可靠Reliable data | 难以直接检测Difficult to detect directly 缺乏合适的电离官能团 Lack of suitable ionizing functional groups | [ |
表面增强拉曼光谱技术 Surface-enhanced Raman spectroscopy | 检测速度快Fast detection speed 检测灵敏度高High detection sensitivity 痕量检测Trace detection | 样品基质复杂,对SERS信号的影响较大 The sample matrix is complex and has a significant impact on SERS signals 目标分析物与纳米活性基底的亲和力问题 也亟待解决 The affinity between the target analyte and the nano-active substrate needs to be solved | [ |
分子生物学技术 Molecular biology technique | 高通量检测High throughput detection 准确性高High accuracy 简单快速Simple and fast | 材料选择具有一定局限性 Material selection has certain limitations | [ |
免疫分析法 Immunoassay | 特异性强Strong specificity 操作简单simple operation 重复性好Good repeatability 易于标准化Easy to standardize | 检测结果与抗体来源和亲和力密切相关 The test results were closely related to the antibody source and affinity 样品中细胞因子可溶性受体的存在可能影响细胞 因子特异性抗体的结合 The presence of cytokine-soluble receptors in the samples may affect the binding of cytokine-specific antibodies | [ |
生物传感器法 Biosensor analysis | 使用固定化生物活性物质作为催化剂 Immobilized bioactive substances are used as catalysts 特异性强Strong specificity 分析速度快Fast analysis 准确性高High accuracy 操作简单Simple operation 成本低Low cost | 稳定性差Poor stability 重复性差Poor reproducibility | [ |
Table 2 Comparison of the advantages and disadvantages of detection techniques for veterinary drug residues in animal-derived foods
检测技术 Detection technique | 优点 Advantage | 缺点 Disadvantage | 参考文献 Reference |
---|---|---|---|
液相色谱法 Liquid chromatography | 分离效率高High separation efficiency 选择性好Good selectivity 检测灵敏度高High detection sensitivity 适用范围广Wide range of application | 设备维护成本昂贵 The equipment is expensive to maintain 检测成本高High detection cost 分析时间长Long analysis time | [ |
气相色谱-质谱法 Gas chromatography- mass spectrometry | 检测速度快Fast detection speed 精准度高High accuracy 成本低Low cost | 主要适用于可气化或衍生的小分子药物 Small molecule drugs are mainly suitable for the gasified or derived drugs 检测后信号输出须矫正 The signal output should be corrected after detection | [ |
液相色谱-串联质谱法 Liquid chromatography- mass spectrometry | 适用范围广Wide scope of application 分离能力强Strong separation ability 数据可靠Reliable data | 难以直接检测Difficult to detect directly 缺乏合适的电离官能团 Lack of suitable ionizing functional groups | [ |
表面增强拉曼光谱技术 Surface-enhanced Raman spectroscopy | 检测速度快Fast detection speed 检测灵敏度高High detection sensitivity 痕量检测Trace detection | 样品基质复杂,对SERS信号的影响较大 The sample matrix is complex and has a significant impact on SERS signals 目标分析物与纳米活性基底的亲和力问题 也亟待解决 The affinity between the target analyte and the nano-active substrate needs to be solved | [ |
分子生物学技术 Molecular biology technique | 高通量检测High throughput detection 准确性高High accuracy 简单快速Simple and fast | 材料选择具有一定局限性 Material selection has certain limitations | [ |
免疫分析法 Immunoassay | 特异性强Strong specificity 操作简单simple operation 重复性好Good repeatability 易于标准化Easy to standardize | 检测结果与抗体来源和亲和力密切相关 The test results were closely related to the antibody source and affinity 样品中细胞因子可溶性受体的存在可能影响细胞 因子特异性抗体的结合 The presence of cytokine-soluble receptors in the samples may affect the binding of cytokine-specific antibodies | [ |
生物传感器法 Biosensor analysis | 使用固定化生物活性物质作为催化剂 Immobilized bioactive substances are used as catalysts 特异性强Strong specificity 分析速度快Fast analysis 准确性高High accuracy 操作简单Simple operation 成本低Low cost | 稳定性差Poor stability 重复性差Poor reproducibility | [ |
类别 Category | 产品 Product | 兽药残留种类 Types of veterinary drug residues |
---|---|---|
蜂产品 Bee products | 蜂蜜Honey | 氯霉素、呋喃西林代谢物、诺氟沙星、甲硝唑、呋喃唑酮 Chloramphenicol, furacillin metabolites, norfloxacin, metronidazole, furazolidone |
蛋产品 Egg products | 鸡蛋Egg | 恩诺沙星、氟苯尼考、磺胺类、甲砜霉素、氯霉素、地美硝唑、金刚烷胺、甲硝唑 Enrofloxacin, flufenicol, sulfonamides, sulfoxamycin, chloramphenicol, dimetronidazole, amantadine, metronidazole |
水产品 Aquatic products | 鱼类Fish | 恩诺沙星、西地泮、磺胺类、呋喃西林代谢物、氧氟沙星、甲硝唑、甲氧苄啶、五氯酚酸钠 Enrofloxacin, cediam, sulfonamides, furacillin metabolites, ofloxacin, metronidazole, trimethoprim, sodium pentachlorophenate |
泥鳅Loach | 恩诺沙星、氧氟沙星Enrofloxacin, ofloxacin | |
畜禽肉 Livestock and | 乌鸡Silkie | 恩诺沙星、金刚烷胺、甲氧苄啶、尼卡巴嗪、呋喃唑酮代谢物、磺胺类 Enrofloxacin, amantadine, trimethoprim, nicarbazine, furazolidone metabolites, sulfonamides |
poultry meat | 牛肉Beef | 克伦特罗、呋喃西林代谢物、地塞米松、五氯酚酸钠 Clenbuterol, furacillin metabolite, dexamethasone, sodium pentachlorophenate |
牛蛙Bullfrog | 恩诺沙星、氯霉素、呋喃西林代谢物Enrofloxacin, chloramphenicol, furacillin metabolites | |
猪肉Pork | 恩诺沙星、氯霉素、氧氟沙星、地塞米松、多西环素 Enrofloxacin, chloramphenicol, ofloxacin, dexamethasone, doxycycline | |
其他肉类 Other meat | 恩诺沙星、孔雀石绿、氯霉素、五氯酚酸钠、多西环素、尼卡巴嗪、呋喃西林代谢物 Enrofloxacin, malachite green, chloramphenicol, sodium pentachlorophenate, doxycycline, nicarbazine, furacillin metabolites |
Table 3 Statistics of veterinary drug residues exceeding the standard from 2020 to 2022
类别 Category | 产品 Product | 兽药残留种类 Types of veterinary drug residues |
---|---|---|
蜂产品 Bee products | 蜂蜜Honey | 氯霉素、呋喃西林代谢物、诺氟沙星、甲硝唑、呋喃唑酮 Chloramphenicol, furacillin metabolites, norfloxacin, metronidazole, furazolidone |
蛋产品 Egg products | 鸡蛋Egg | 恩诺沙星、氟苯尼考、磺胺类、甲砜霉素、氯霉素、地美硝唑、金刚烷胺、甲硝唑 Enrofloxacin, flufenicol, sulfonamides, sulfoxamycin, chloramphenicol, dimetronidazole, amantadine, metronidazole |
水产品 Aquatic products | 鱼类Fish | 恩诺沙星、西地泮、磺胺类、呋喃西林代谢物、氧氟沙星、甲硝唑、甲氧苄啶、五氯酚酸钠 Enrofloxacin, cediam, sulfonamides, furacillin metabolites, ofloxacin, metronidazole, trimethoprim, sodium pentachlorophenate |
泥鳅Loach | 恩诺沙星、氧氟沙星Enrofloxacin, ofloxacin | |
畜禽肉 Livestock and | 乌鸡Silkie | 恩诺沙星、金刚烷胺、甲氧苄啶、尼卡巴嗪、呋喃唑酮代谢物、磺胺类 Enrofloxacin, amantadine, trimethoprim, nicarbazine, furazolidone metabolites, sulfonamides |
poultry meat | 牛肉Beef | 克伦特罗、呋喃西林代谢物、地塞米松、五氯酚酸钠 Clenbuterol, furacillin metabolite, dexamethasone, sodium pentachlorophenate |
牛蛙Bullfrog | 恩诺沙星、氯霉素、呋喃西林代谢物Enrofloxacin, chloramphenicol, furacillin metabolites | |
猪肉Pork | 恩诺沙星、氯霉素、氧氟沙星、地塞米松、多西环素 Enrofloxacin, chloramphenicol, ofloxacin, dexamethasone, doxycycline | |
其他肉类 Other meat | 恩诺沙星、孔雀石绿、氯霉素、五氯酚酸钠、多西环素、尼卡巴嗪、呋喃西林代谢物 Enrofloxacin, malachite green, chloramphenicol, sodium pentachlorophenate, doxycycline, nicarbazine, furacillin metabolites |
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[1] | XIA Wei, LI Zuguang, YANG Hua, JI Xiaofeng, WANG Jianmei, QIAN Mingrong. Study on sulfamonomethoxine residue in eggs [J]. , 2019, 31(1): 155-160. |
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