Comparative study on inactivated vaccines and similar products against Escherichia coli and Clostridium perfringens

doi:10.3969/j.issn.1004-1524.20240914

Acta Agriculturae Zhejiangensis ›› 2025, Vol. 37 ›› Issue (11): 2258-2264.DOI: 10.3969/j.issn.1004-1524.20240914

• Animal Science • Previous Articles Next Articles

Comparative study on inactivated vaccines and similar products against Escherichia coli and Clostridium perfringens

LIU Zewen(), YUAN Fangyan, LIU Wei, GAO Ting, YANG Keli, GUO Rui, LI Chang, WU Qiong, ZHU Jiajia, TIAN Yongxiang, ZHOU Danna()

Key Laboratory of Prevention and Control Agents for Animal Bacteriosis, Ministry of Agriculture and Rural Affairs, Hubei Provincial Key Laboratory of Livestock and Poultry of Pathogen Microbiology, Institute of Animal Husbandry and Veterinary, Hubei Academy of Agricultural Sciences, Wuhan 430064,China

Received:2024-10-25 Online:2025-11-25 Published:2025-12-08

Abstract

Abstract:

In order to compare the safety and immune efficacy of the dual inactivated vaccine for piglet Escherichia coli disease and Clostridium perfringens(bivalent vaccine) developed by the author team with similar dual vaccine and individual vaccines, the inactivated vaccine in our laboratory was compared with the trivalent inactivated vaccine against Escherichia coli and the inactivated vaccine against C-type Clostridium perfringens produced by domestic biopharmaceutical companies, as well as the combined inactivated vaccine produced by a foreign company. Comparative experiments were conducted on traits, sterility testing, safety, and immune protection effects under the same conditions. The results showed that the inactivated vaccines were qualified in terms of characteristics and sterility compared with domestic and imported vaccines, and all vaccines were safe for mice and weaned piglets. The protection rate of the inactivated vaccine against various strains of Escherichia coli and C-type Clostridium perfringens achieved 80%-100%, with no significant difference in efficacy compared with various single vaccines and imported vaccines of the same type. The comparison results showed that the quality of the inactivated vaccine developed by author team is not lower than that of each monovalent vaccine, trivalent vaccine, and similar imported vaccine.

Key words: Escherichia coli, C-type Clostridium perfringens, inactivated vaccine, similar vaccine, safety test, immune protection test

CLC Number:

LIU Zewen, YUAN Fangyan, LIU Wei, GAO Ting, YANG Keli, GUO Rui, LI Chang, WU Qiong, ZHU Jiajia, TIAN Yongxiang, ZHOU Danna. Comparative study on inactivated vaccines and similar products against Escherichia coli and Clostridium perfringens[J]. Acta Agriculturae Zhejiangensis, 2025, 37(11): 2258-2264.

Figures/Tables 5

References 27

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组别 Group	接种数量 Vaccination quantity	接种剂量 Vaccination dose/mL	结果 Results
二联苗Bivalent vaccine	5	0.5	5/5健活Living
E苗E vaccine	5	0.5	5/5健活Living
C苗C vaccine	5	0.5	5/5健活Living
进口二联苗Imported bivalent vaccine	5	0.5	5/5健活Living

组别 Group	接种数量 Vaccination quantity	接种剂量 Vaccination dose/mL	结果 Results
二联苗Bivalent vaccine	5	0.5	5/5健活Living
E苗E vaccine	5	0.5	5/5健活Living
C苗C vaccine	5	0.5	5/5健活Living
进口二联苗Imported bivalent vaccine	5	0.5	5/5健活Living

组别 Group	接种数量 Vaccination quantity	接种剂量 Vaccination dose/mL	结果 Results
二联苗Bivalent vaccine	5	4	5/5健活Living
E苗E vaccine	5	8	5/5健活Living
C苗C vaccine	5	8	5/5健活Living
进口二联苗Imported bivalent vaccine	5	4	5/5健活Living
对照组Control	5	0	5/5健活Living

组别 Group	接种数量 Vaccination quantity	接种剂量 Vaccination dose/mL	结果 Results
二联苗Bivalent vaccine	5	4	5/5健活Living
E苗E vaccine	5	8	5/5健活Living
C苗C vaccine	5	8	5/5健活Living
进口二联苗Imported bivalent vaccine	5	4	5/5健活Living
对照组Control	5	0	5/5健活Living

组别 Group	疫苗注射不同时间(天)后仔猪体温Body temperature of weaned piglets after different vaccine injection time (days)
组别 Group	1	2	3	4	5	6	7	8	9	10	11	12	13	14
二联苗Bivalent vaccine	39.0	39.0	38.9	39.0	38.9	39.3	39.2	39.1	38.8	39.0	39.1	38.8	39.0	39.1
E苗E vaccine	39.1	39.1	38.9	38.8	39.1	39.2	39.1	39.4	39.1	39.0	39.1	39.0	39.0	39.2
C苗C vaccine	38.9	39.2	39.0	38.7	39.1	39.2	39.4	39.0	39.2	38.8	39.0	39.2	39.2	39.0
进口二联苗 Imported bivalent vaccine	39.1	39.2	39.0	39.0	39.0	39.2	39.0	38.9	39.1	39.1	38.9	39.4	39.1	38.9
对照组Control	39.0	38.9	39.1	39.2	39.1	39.1	38.9	39.2	39.3	39.1	38.9	39.3	39.2	39.1

Comparative study on inactivated vaccines and similar products against Escherichia coli and Clostridium perfringens

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组别 Group	纤毛类型 Cilia type	攻毒菌株 Challenge strains	攻毒量 Challenge amount/CFU	攻毒数 Challenge number	发病数 Diseased number	发病保护率 Disease protection rate/%
二联苗Bivalent vaccine	K88	C83549	1.5×10¹⁰	5	0	100
	K99	C83644	1.5×10¹⁰	5	1	80
	987P	C83710	1.5×10¹⁰	5	0	100
E苗E vaccine	K88	C83549	1.5×10¹⁰	5	0	100
	K99	C83644	1.5×10¹⁰	5	1	80
	987P	C83710	1.5×10¹⁰	5	1	80
进口二联苗 Imported bivalent vaccine	K88	C83549	1.5×10¹⁰	5	0	100
	K99	C83644	1.5×10¹⁰	5	1	80
	987P	C83710	1.5×10¹⁰	5	1	80
非免疫攻毒对照组 Non immune challenge control group	—	C83549	1.5×10¹⁰	5	5	0
		C83644	1.5×10¹⁰	5	5	0
		C83710	1.5×10¹⁰	5	5	0
正常对照组Normal control	—	—	—	—	5	—

组别 Group	毒素攻击剂量 Toxin challenge dose	攻毒数 Challenge number	死亡数 Death number	死亡保护率 Death protection rate/%
二联苗Bivalent vaccine	1个仔猪最小致死剂量Minimum lethal dose for 1 piglet	5	0	100
E苗E vaccineImported bivalent vaccine	1个仔猪最小致死剂量Minimum lethal dose for 1 piglet	5	0	100
进口二联苗 Imported bivalent vaccine	1个仔猪最小致死剂量Minimum lethal dose for 1 piglet	5	0	100
非免疫攻毒对照组Non immune challenge control group	1个仔猪最小致死剂量Minimum lethal dose for 1 piglet	5	5	0
正常对照组Normal control	—	5	0	—

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