Effect of biogas slurry application with chemical fertilizer reduction on water bamboo production and soil improvement

doi:10.3969/j.issn.1004-1524.20250027

Acta Agriculturae Zhejiangensis ›› 2026, Vol. 38 ›› Issue (1): 126-135.DOI: 10.3969/j.issn.1004-1524.20250027

• Environmental Science • Previous Articles Next Articles

Effect of biogas slurry application with chemical fertilizer reduction on water bamboo production and soil improvement

BA Shihao¹^,²(), TONG Wenbin³^,^*(), YANG Haijun³, JIANG Jianfeng³, LI Zichuan¹, WU Chunyan², TANG Xu², CHAI Yanjun¹, LI Yan²^,^*()

1. Key Laboratory of Recycling and Ecotreatment of Waste Biomass of Zhejiang Province, School of Environment and Natural Resources, Zhejiang University of Science and Technology, Hangzhou 310023, China
2. Institute of Environment, Resource, Soil and Fertilizer, Zhejiang Academy of Agricultural Sciences, Hangzhou 310021, China
3. Qujiang District Agriculture and Rural Affairs Bureau, Quzhou 324022, Zhejiang, China

Received:2025-01-06 Online:2026-01-25 Published:2026-02-11

Abstract

Abstract:

To solve the problems of nutrient loss and soil degradation caused by excessive application of chemical fertilizer in water bamboo (Zizania latifolia) fields, the effect of biogas slurry application with chemical fertilizer reduction on water bamboo production was explored. There were six treatments in this experiment, including blank control (CK, no fertilizer was applied), control (NPK, conventional fertilization), and test treatments (biogas slurry instead of 25%, 50%, 75%, 100% equivalent nitrogen fertilizer, denoted as 25%NP₁K₁Z₁, 50%NP₂K₂Z₂, 75%NP₃K₃Z₃, 100%NP₄K₄Z₄, respectively). The effects of these treatments were monitored on water bamboo yield and quality, soil basic physical and chemical properties, soil microbial biomass and enzyme activity. It was shown that there was no significant difference in water bamboo yield among treatments excluding CK. Compared with NPK treatment, the content of crude protein and vitamin C of water bamboo was significantly (p<0.05) increased under the treatments of 75%NP₃K₃Z₃ and 100%NP₄K₄Z₄, while the nitrate content of water bamboo was significantly decreased. Besides, the content of soil available phosphorus, available potassium, microbial biomass carbon and microbial biomass nitrogen and the activities of soil sucrase, urease and catalase under these two treatments were also significantly higher than those under NPK treatment. In conclusion, 75%NP₃K₃Z₃ treatment was the most suitable treatment under the test condition, as it not only improved the quality of water bamboo without yield reduction, but also improved soil properties, which achieved the goal of reducing chemical fertilizer application and increasing utilization efficiency of biogas slurry.

Key words: water bamboo (Zizania latifolia), biogas slurry, reduced chemical fertilizer application rate, soil improvement, reducing fertilizer use and increasing use efficiency

CLC Number:

S158.1

BA Shihao, TONG Wenbin, YANG Haijun, JIANG Jianfeng, LI Zichuan, WU Chunyan, TANG Xu, CHAI Yanjun, LI Yan. Effect of biogas slurry application with chemical fertilizer reduction on water bamboo production and soil improvement[J]. Acta Agriculturae Zhejiangensis, 2026, 38(1): 126-135.

Figures/Tables 7

References 37

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处理 Treatment	茭长/cm Length of water bamboo shoot/cm	茭宽/cm Width of water bamboo shoot/cm	叶长/cm Length of leaf/cm	叶宽/cm Width of leaf/cm	壳茭重/g Gross weight of water bamboo shoot/g	净茭重/g Net weight of water bamboo shoot/g
CK	16.15±2.01 c	4.25±1.11 a	1.63±0.21 a	2.76±0.15 c	128.63±20.11 b	96.02±9.89 b
NPK	18.90±1.05 b	4.44±0.56 a	1.74±0.35 a	3.65±0.99 b	140.24±23.12 a	105.48±15.23 ab
25%NP₁K₁Z₁	18.18±2.22 b	4.46±0.86 a	1.77±0.40 a	3.66±0.81 b	137.73±15.50 a	112.66±8.96 a
50%NP₂K₂Z₂	20.09±3.15 a	4.43±0.29 a	1.79±0.11 a	3.75±0.63 b	141.65±29.91 a	114.57±19.6 1a
75%NP₃K₃Z₃	20.16±3.76 a	4.43±0.25 a	1.81±0.19 a	3.69±0.89 b	145.25±10.69 a	115.12±20.11 a
100%NP₄K₄Z₄	20.50±2.56 a	4.54±1.02 a	1.87±0.26 a	4.02±0.88 a	148.63±12.22 a	115.29±11.50 a

处理 Treatment	茭长/cm Length of water bamboo shoot/cm	茭宽/cm Width of water bamboo shoot/cm	叶长/cm Length of leaf/cm	叶宽/cm Width of leaf/cm	壳茭重/g Gross weight of water bamboo shoot/g	净茭重/g Net weight of water bamboo shoot/g
CK	16.15±2.01 c	4.25±1.11 a	1.63±0.21 a	2.76±0.15 c	128.63±20.11 b	96.02±9.89 b
NPK	18.90±1.05 b	4.44±0.56 a	1.74±0.35 a	3.65±0.99 b	140.24±23.12 a	105.48±15.23 ab
25%NP₁K₁Z₁	18.18±2.22 b	4.46±0.86 a	1.77±0.40 a	3.66±0.81 b	137.73±15.50 a	112.66±8.96 a
50%NP₂K₂Z₂	20.09±3.15 a	4.43±0.29 a	1.79±0.11 a	3.75±0.63 b	141.65±29.91 a	114.57±19.6 1a
75%NP₃K₃Z₃	20.16±3.76 a	4.43±0.25 a	1.81±0.19 a	3.69±0.89 b	145.25±10.69 a	115.12±20.11 a
100%NP₄K₄Z₄	20.50±2.56 a	4.54±1.02 a	1.87±0.26 a	4.02±0.88 a	148.63±12.22 a	115.29±11.50 a

处理 Treatment	总糖含量/ (g·kg^-1) Total sugar content/(g·kg^-1)	粗蛋白含量/ (g·kg^-1) Crude protein content/(g·kg^-1)	膳食纤维含量/ (g·kg^-1) Dietary fiber content/(g·kg^-1)	维生素C含量/ (mg·kg^-1) Vitamin C content/(mg·kg^-1)	硝酸盐含量/ (mg·kg^-1) Nitrate content/ (mg·kg^-1)
CK	43.85±4.21 a	10.15±0.42 c	23.82±1.65 a	179.16±7.40 c	506.5±43.7 c
NPK	45.60±2.69 a	11.02±1.03 bc	22.43±2.42 a	209.78±13.42 b	627.3±60.3 a
25%NP₁K₁Z₁	46.75±0.80 a	11.67±0.91 bc	23.28±2.46 a	215.92±23.12 b	578.9±48.9 b
50%NP₂K₂Z₂	47.11±5.14 a	12.17±1.95 b	23.05±2.20 a	222.15±21.21 b	564.3±55.2 b
75%NP₃K₃Z₃	47.17±3.47 a	13.66±1.07 a	22.51±1.39 a	239.52±19.74 a	512.9±1.29 c
100%NP₄K₄Z₄	48.90±2.41 a	13.96±1.31 a	22.43±2.41 a	240.28±22.80 a	524.3±26.7 c

处理 Treatment	总糖含量/ (g·kg^-1) Total sugar content/(g·kg^-1)	粗蛋白含量/ (g·kg^-1) Crude protein content/(g·kg^-1)	膳食纤维含量/ (g·kg^-1) Dietary fiber content/(g·kg^-1)	维生素C含量/ (mg·kg^-1) Vitamin C content/(mg·kg^-1)	硝酸盐含量/ (mg·kg^-1) Nitrate content/ (mg·kg^-1)
CK	43.85±4.21 a	10.15±0.42 c	23.82±1.65 a	179.16±7.40 c	506.5±43.7 c
NPK	45.60±2.69 a	11.02±1.03 bc	22.43±2.42 a	209.78±13.42 b	627.3±60.3 a
25%NP₁K₁Z₁	46.75±0.80 a	11.67±0.91 bc	23.28±2.46 a	215.92±23.12 b	578.9±48.9 b
50%NP₂K₂Z₂	47.11±5.14 a	12.17±1.95 b	23.05±2.20 a	222.15±21.21 b	564.3±55.2 b
75%NP₃K₃Z₃	47.17±3.47 a	13.66±1.07 a	22.51±1.39 a	239.52±19.74 a	512.9±1.29 c
100%NP₄K₄Z₄	48.90±2.41 a	13.96±1.31 a	22.43±2.41 a	240.28±22.80 a	524.3±26.7 c

处理 Treatment	pH值 pH value	有机质含量/ (g·kg^-1) Organic matter content/ (g·kg^-1)	全氮含量/ (g·kg^-1) Total nitrogen content/ (g·kg^-1)	全磷含量/ (g·kg^-1) Total phosphorus content/ (g·kg^-1)	全钾含量/ (g·kg^-1) Total potassium content/ (g·kg^-1)	碱解氮含量/ (mg·kg^-1) Alkali hydrolyzable nitrogen content/ (mg·kg^-1)	有效磷含量/ (mg·kg^-1) Available phosphorus content/ (mg·kg^-1)	速效钾含量/ (mg·kg^-1) Available potassium content/ (mg·kg^-1)
CK	5.17± 0.71 a	18.24± 1.01 a	1.02± 0.09 a	0.71± 0.08 a	13.6± 1.10 a	120.9± 11.3 c	31.29± 2.31 b	78.72± 7.42 b
NPK	5.20± 0.34 a	19.96± 1.45 a	1.18± 0.12 a	0.74± 0.06 a	14.5± 0.89 a	149.5± 13.2 a	32.08± 0.27 b	73.87± 6.29 b
25%NP₁K₁Z₁	5.36± 0.42 a	20.15± 2.14 a	1.17± 0.04 a	0.74± 0.03 a	14.8± 0.72 a	134.2± 10.5 b	33.16± 1.19 b	70.78± 4.41 b
50%NP₂K₂Z₂	5.38± 0.26 a	20.36± 1.25 a	1.20± 0.11 a	0.76± 0.06 a	15.0± 1.21 a	142.4± 8.61 a	33.10± 3.26 b	87.24± 7.03 a
75%NP₃K₃Z₃	5.37± 0.72 a	21.02± 0.58 a	1.25± 0.07 a	0.83± 0.05 a	15.8± 0.97 a	151.2± 12.7 a	43.34± 2.32 a	90.55± 8.95 a
100%NP₄K₄Z₄	5.30± 0.52 a	20.21± 1.42 a	1.24± 0.13 a	0.81± 0.07 a	15.2± 0.47 a	153.5± 14.2 a	42.12± 3.37 a	91.21± 7.95 a

Effect of biogas slurry application with chemical fertilizer reduction on water bamboo production and soil improvement

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处理 Treatment	Cu含量 Cu content	Cr含量 Cr content	Cd含量 Cd content	Ni含量 Ni content	Pb含量 Pb content	Zn含量 Zn content
CK	27.43±3.29 a	16.71±1.84 a	0.24±0.02 a	26.02±1.41 a	30.02±0.31 a	68.24±6.99 a
NPK	30.12±2.43 a	19.01±1.21 a	0.18±0.05 a	25.14±2.75 a	32.56±1.53 a	68.80±9.21 a
25%NP₁K₁Z₁	26.47±2.26 a	17.23±1.62 a	0.23±0.04 a	27.32±2.24 a	32.39±1.42 a	73.24±8.25 a
50%NP₂K₂Z₂	30.08±2.61 a	20.12±1.86 a	0.17±0.02 a	24.98±2.23 a	32.16±0.89 a	73.16±6.43 a
75%NP₃K₃Z₃	27.56±1.43 a	18.48±1.31 a	0.25±0.05 a	26.56±1.76 a	33.79±1.85 a	73.42±5.68 a
100%NP₄K₄Z₄	30.74±3.01 a	19.56±2.01 a	0.19±0.08 a	28.43±1.56 a	32.58±1.49 a	73.95±0.88 a

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