Pathogens identification of pepper anthracnose and fungicide screening

doi:10.3969/j.issn.1004-1524.20250101

Acta Agriculturae Zhejiangensis ›› 2025, Vol. 37 ›› Issue (11): 2315-2324.DOI: 10.3969/j.issn.1004-1524.20250101

• Plant Protection • Previous Articles Next Articles

Pathogens identification of pepper anthracnose and fungicide screening

LIU Yating¹(), WU Lidong¹^,², QIU Yinhui¹, CHEN Guoyu¹, ZHONG Liuqing¹^,^*()

1. Sanming Academy of Agricultural Sciences, Sanming 365509, Fujian, China
2. Fujian Key Laboratory of Crop Genetic Improvement and Innovative Utilization of Mountain Area, Sanming 365509, Fujian, China

Received:2025-02-11 Online:2025-11-25 Published:2025-12-08

Abstract

Abstract:

To identify the causal pathogen of pepper anthracnose in Sanming City, Fujian Province, China, and to screen out effective fungicides for disease control, diseased fruits were collected from major production areas. The pathogen was isolated and purified by tissue separation, and was identified through morphological characterization and multilocus phylogenetic analysis (ITS, ACT, CHS-1, TUB2, GAPDH and CAL). Antifungal activities of 10 fungicides were evaluated by using the mycelial growth inhibition method, as well as their field efficacy. It was shown that the pathogen was identified as Colletotrichum siamense. In vitro toxicity tests revealed that the 24% guazatine pyraclostrobin wettable powder exhibited the highest inhibitory effect, with a median effective concentration (EC₅₀) of 0.176 mg·L^-1, which was followed by 30% difenoconazole pyraclostrobin suspension concentrate (EC₅₀ of 0.263 mg·L^-1), 60% azoxystrobin + tebuconazole water dispersible granules (EC₅₀ of 0.367 mg·L^-1), 17% azoxystrobin epoxiconazole suspension concentrate (EC₅₀ of 0.371 mg·L^-1). In field trials, 30% difenoconazole pyraclostrobin suspension concentrate and 50% prochloraz-copper salt suspension concentrate demonstrated the highest control efficacy of 91.51% and 90.83%, respectively, on the 7th day after the third application, which was followed by 24% guazatine pyraclostrobin wettable powder. Therefore, 30% difenoconazole pyraclostrobin suspension concentrate and 24% guazatine pyraclostrobin wettable powder could be used as preferred agents to control pepper anthracnose in peppers, and should be used alternately.

Key words: pepper anthracnose, Colletotrichum siamense, fungicide screening, control efficacy

CLC Number:

S436.418.11

LIU Yating, WU Lidong, QIU Yinhui, CHEN Guoyu, ZHONG Liuqing. Pathogens identification of pepper anthracnose and fungicide screening[J]. Acta Agriculturae Zhejiangensis, 2025, 37(11): 2315-2324.

Figures/Tables 6

References 31

[1]	邹学校, 马艳青, 戴雄泽, 等. 辣椒在中国的传播与产业发展[J]. 园艺学报, 2020, 47(9): 1715-1726.
	ZOU X X, MA Y Q, DAI X Z, et al. Spread and industry development of pepper in China[J]. Acta Horticulturae Sinica, 2020, 47(9): 1715-1726. (in Chinese with English abstract)
[2]	THAN P P, JEEWON R, HYDE K D, et al. Characterization and pathogenicity of Colletotrichum species associated with anthracnose on chilli (Capsicum spp.) in Thailand[J]. Plant Pathology, 2008, 57(3): 562-572.
[3]	SAXENA A, RAGHUWANSHI R, GUPTA V K, et al. Chilli anthracnose: the epidemiology and management[J]. Frontiers in Microbiology, 2016, 7: 1527.
[4]	TORRES-CALZADA C, TAPIA-TUSSELL R, HIGUERA-CIAPARA I, et al. Characterization of Colletotrichum truncatum from papaya, pepper and physic nut based on phylogeny, morphology and pathogenicity[J]. Plant Pathology, 2018, 67(4): 821-830.
[5]	NOOR N M, ZAKARIA L. Identification and characterization of Colletotrichum spp. associated with chili anthracnose in peninsular Malaysia[J]. European Journal of Plant Pathology, 2018, 151(4): 961-973.
[6]	张河庆, 宋占峰, 韩帅, 等. 四川辣椒炭疽病菌鉴定及防治药剂筛选研究[J]. 中国蔬菜, 2024(4): 93-99.
	ZHANG H Q, SONG Z F, HAN S, et al. Studies on identification of Capsicum anthracnose in Sichuan and screening of control agents[J]. China Vegetables, 2024(4): 93-99. (in Chinese with English abstract)
[7]	周志成, 孙海, 肖仲久, 等. 辣椒炭疽病病原分离鉴定及对杀菌剂敏感性测定[J]. 华南农业大学学报, 2023, 44(3): 430-437.
	ZHOU Z C, SUN H, XIAO Z J, et al. Identification and fungicide sensitivity of pathogen causing anthracnose of pepper[J]. Journal of South China Agricultural University, 2023, 44(3): 430-437. (in Chinese with English abstract)
[8]	郑婕, 庄远航, 郭梓琪, 等. 南雄辣椒炭疽病菌的分离鉴定与药剂筛选[J]. 中国蔬菜, 2022(10): 58-65.
	ZHENG J, ZHUANG Y H, GUO Z Q, et al. Isolation, identification and fungicide screening of Capsicum anthracnose pathogen in Nanxiong County[J]. China Vegetables, 2022(10): 58-65. (in Chinese with English abstract)
[9]	荆卓琼, 郭致杰, 孙倩, 等. 甘肃省辣椒炭疽病病原菌鉴定[J]. 北方园艺, 2024(15): 1-7.
	JING Z Q, GUO Z J, SUN Q, et al. Identification of pathogen causing anthracnose of pepper (Capsicum annuum L.) in Gansu Province[J]. Northern Horticulture, 2024(15): 1-7. (in Chinese with English abstract)
[10]	陈剑山, 郑服丛. ITS序列分析在真菌分类鉴定中的应用[J]. 安徽农业科学, 2007, 35(13): 3785-3786.
	CHEN J S, ZHENG F C. Application of ITS sequences in fungi classification and identification[J]. Journal of Anhui Agricultural Sciences, 2007, 35(13): 3785-3786. (in Chinese with English abstract)
[11]	CARBONE I, KOHN L M. A method for designing primer sets for speciation studies in filamentous ascomycetes[J]. Mycologia, 1999, 91(3): 553-556.
[12]	GLASS N L, DONALDSON G C. Development of primer sets designed for use with the PCR to amplify conserved genes from filamentous ascomycetes[J]. Applied and Environmental Microbiology, 1995, 61(4): 1323-1330.
[13]	TEMPLETON M D, RIKKERINK E H A, SOLON S L, et al. Cloning and molecular characterization of the glyceraldehyde-3-phosphate dehydrogenase-encoding gene and cDNA from the plant pathogenic fungus Glomerella cingulata[J]. Gene, 1992, 122(1): 225-230.
[14]	WEIR B S, JOHNSTON P R, DAMM U. The Colletotrichum gloeosporioides species complex[J]. Studies in Mycology, 2012, 73: 115-180.
[15]	吴立东, 罗英, 曾绍贵, 等. 朝天椒新品种明椒9号的选育[J]. 中国蔬菜, 2018(9): 70-72.
	WU L D, LUO Y, ZENG S G, et al. A new hot pepper F1 hybrid: ‘Mingjiao No.9’[J]. China Vegetables, 2018(9): 70-72. (in Chinese with English abstract)
[16]	张明良. 辣椒炭疽病的发生与防治[J]. 安徽农业, 2000(2): 21.
	ZHANG M L. Occurrence and control of pepper anthracnose[J]. Anhui Agriculture, 2000(2): 21. (in Chinese)
[17]	DIAO Y Z, ZHANG C, LIU F, et al. Colletotrichum species causing anthracnose disease of chili in China[J]. Persoonia, 2017, 38: 20-37.
[18]	冯乐乐, 竹龙鸣, 谢华, 等. 浙江省鲜食大豆炭疽病病原分离及抗性鉴定[J]. 植物病理学报, 2021, 51(6): 840-849.
	FENG L L, ZHU L M, XIE H, et al. Identification of the pathogen of vegetable soybean anthracnose in Zhejiang Province and evaluation of soybean cultivars for resistance to Colletotrichum truncatum[J]. Acta Phytopathologica Sinica, 2021, 51(6): 840-849. (in Chinese with English abstract)
[19]	王秋月, 黄娟, 李四光, 等. 重庆高粱炭疽病病原菌鉴定及防治药剂筛选[J]. 植物保护, 2024, 50(4): 323-330.
	WANG Q Y, HUANG J, LI S G, et al. Identification of the pathogen of sorghum anthracnose in Chongqing and fungicides screening[J]. Plant Protection, 2024, 50(4): 323-330. (in Chinese with English abstract)
[20]	侯圣凡, 华战迎, 刘峻杰, 等. 我国草莓胶孢炭疽菌的多基因联合鉴定与致病性分析[J]. 中国农业大学学报, 2022, 27(4): 82-94.
	HOU S F, HUA Z Y, LIU J J, et al. Multi-gene joint identification and pathogenicity analysis of Colletotrichum gloeosporioides complex of strawberry in China[J]. Journal of China Agricultural University, 2022, 27(4): 82-94. (in Chinese with English abstract)
[21]	李少卡, 赵亚, 王祥和, 等. 海南荔枝炭疽病病原菌鉴定及遗传多样性分析[J]. 农业生物技术学报, 2021, 29(4): 673-687.
	LI S K, ZHAO Y, WANG X H, et al. Identification and genetic diversity analysis of Litchi chinensis Colletotrichum spp. in Hainan[J]. Journal of Agricultural Biotechnology, 2021, 29(4): 673-687. (in Chinese with English abstract)
[22]	张童, 姚立萍, 胡玉慈, 等. 甜柿炭疽病病原菌的鉴定及其防治药剂的筛选[J]. 福建农林大学学报(自然科学版), 2020, 49(5): 589-596.
	ZHANG T, YAO L P, HU Y C, et al. Pathogen identification of sweet persimmon anthracnose and screening of the fungicides[J]. Journal of Fujian Agriculture and Forestry University(Natural Science Edition), 2020, 49(5): 589-596. (in Chinese with English abstract)
[23]	石文波, 黄楚平, 陈宇航, 等. 纳米氢氧化铜对茶炭疽病菌的抑制活性及作用机制[J]. 农药学学报, 2024, 26(5): 962-973.
	SHI W B, HUANG C P, CHEN Y H, et al. Antimicrobial activity and mechanism of nano-Cu(OH)₂ against tea anthracnose pathogen[J]. Chinese Journal of Pesticide Science, 2024, 26(5): 962-973. (in Chinese with English abstract)
[24]	任立超, 谢昀烨, 施鹏程, 等. 甜柿炭疽病病原种类及生物学特性比较[J]. 果树学报, 2023, 40(2): 340-349.
	REN L C, XIE Y Y, SHI P C, et al. Pathogen identification and biological characteristics of sweet persimmon anthracnose causing by Colletotrichum species[J]. Journal of Fruit Science, 2023, 40(2): 340-349. (in Chinese with English abstract)
[25]	杨洪俊, 张旭, 陈佳佳, 等. 草莓炭疽病病原鉴定及拮抗菌株筛选[J]. 江苏农业科学, 2024, 52(3): 147-152.
	YANG H J, ZHANG X, CHEN J J, et al. Identification of pathogen and screening of antagonistic strains for strawberry anthracnose[J]. Jiangsu Agricultural Sciences, 2024, 52(3): 147-152. (in Chinese with English abstract)
[26]	刘在哲, 葛磊, 季延平, 等. 红肉苹果炭疽病病原鉴定及其生长适应性研究[J]. 山东农业大学学报(自然科学版), 2021, 52(5): 713-722.
	LIU Z Z, GE L, JI Y P, et al. Identification and characterization of Colletotrichum siamense from anthracnose disease of red-fleshed apple and adapation[J]. Journal of Shandong Agricultural University(Natural Science Edition), 2021, 52(5): 713-722. (in Chinese with English abstract)
[27]	李晶, 仇芳, 张超, 等. 海南白沙和儋州油梨叶部及果实炭疽病菌的鉴定[J]. 果树学报, 2023, 40(7): 1443-1454.
	LI J, QIU F, ZHANG C, et al. Identification of pathogen species of avocado (Persea americana Mill.) leaf and fruit anthracnose in Baisha and Danzhou, Hainan[J]. Journal of Fruit Science, 2023, 40(7): 1443-1454. (in Chinese with English abstract)
[28]	苏奇倩, 何鹏搏, 何俊, 等. 中国滇西地区葡萄炭疽病病原菌的鉴定及其杀菌剂筛选[J]. 西南大学学报(自然科学版), 2024, 46(8): 31-44.
	SU Q Q, HE P B, HE J, et al. Identification of the pathogens causing grape anthracnose in west Yunnan, China and selection of effective fungicides[J]. Journal of Southwest University(Natural Science Edition), 2024, 46(8): 31-44. (in Chinese with English abstract)
[29]	王成, 赵丹阳, 扈丽丽, 等. 苹婆炭疽病病原菌生物学特性及室内毒力测定[J]. 林业与环境科学, 2023, 39(4): 21-26.
	WANG C, ZHAO D Y, HU L L, et al. Studies on biological characteristics and lab toxicity test of leaf pathogen(Colletotrichum siamense) on Sterculia monosperma[J]. Forestry and Environmental Science, 2023, 39(4): 21-26. (in Chinese with English abstract)
[30]	李文, 何月秋, 王佳莹, 等. 百日草炭疽病病原菌的分离鉴定及防治药剂筛选[J]. 农药学学报, 2021, 23(2): 341-347.
	LI W, HE Y Q, WANG J Y, et al. Isolation and identification of pathogen causing anthracnose on Zinnia elegans Jacq. and fungicides screening[J]. Chinese Journal of Pesticide Science, 2021, 23(2): 341-347. (in Chinese with English abstract)
[31]	徐丹丹, 石力允, 林泽勉, 等. 美丽崖豆藤炭疽病病原鉴定及防治药剂的室内毒力测定[J]. 华南农业大学学报, 2021, 42(4): 63-69.
	XU D D, SHI L Y, LIN Z M, et al. Pathogen identification of anthracnose disease on Millettia speciosa and indoor determination of fungicide toxicity[J]. Journal of South China Agricultural University, 2021, 42(4): 63-69. (in Chinese with English abstract)

目标基因/序列 Target gene/ sequence	正向引物序列(5'→3') Forward primer sequence(5'→3')	反向引物序列(5'→3') Reverse primer sequence(5'→3')	退火温度 Annealing temperature/℃	扩增片段大小 Amplified fragment size/bp
ITS	TCCTCCGCTTATTGATATGC	GGAAGTAAAAGTCGTAACAAGG	56	540
ACT	ATGTGCAAGGCCGGTTTCGC	TACGAGTCCTTCTGGCCCAT	59	240
CHS-1	TGGGGCAAGGATGCTTGGAAGAAG	TGGAAGAACCATCTGTGAGAGTTG	56	280
TUB2	GGTAACCAAATCGGTGCTGCTTTC	ACCCTCAGTGTAGTGACCCTTGGC	52	490
GAPDH	GCCGTCAACGACCCCTTCATTGA	GGGTGGAGTCGTACTTGAGCATGT	59	980
CAL	GAATTCAAGGAGGCCTTCTC	CTTCTGCACTGAGCTGGAC	50	240

目标基因/序列 Target gene/ sequence	正向引物序列(5'→3') Forward primer sequence(5'→3')	反向引物序列(5'→3') Reverse primer sequence(5'→3')	退火温度 Annealing temperature/℃	扩增片段大小 Amplified fragment size/bp
ITS	TCCTCCGCTTATTGATATGC	GGAAGTAAAAGTCGTAACAAGG	56	540
ACT	ATGTGCAAGGCCGGTTTCGC	TACGAGTCCTTCTGGCCCAT	59	240
CHS-1	TGGGGCAAGGATGCTTGGAAGAAG	TGGAAGAACCATCTGTGAGAGTTG	56	280
TUB2	GGTAACCAAATCGGTGCTGCTTTC	ACCCTCAGTGTAGTGACCCTTGGC	52	490
GAPDH	GCCGTCAACGACCCCTTCATTGA	GGGTGGAGTCGTACTTGAGCATGT	59	980
CAL	GAATTCAAGGAGGCCTTCTC	CTTCTGCACTGAGCTGGAC	50	240

杀菌剂 Fungicide	回归方程 Regression equation	R²	EC₅₀/(mg·L^-1)
杀菌剂 Fungicide	回归方程 Regression equation	R²	平均值Mean	95%CI
45%吡醚·甲硫灵悬浮剂	y=1.481+2.147x	0.999 5	0.690	0.635~0.751
45% Pyraclostrobin + thiophanate-methyl suspension concentrate
60%唑醚·戊唑醇水分散粒剂	y=1.196+3.261x	0.990 3	0.367	0.331~0.410
60% Azoxystrobin+tebuconazole water dispersible granules
50%咪鲜胺铜盐悬浮剂	y=1.594+3.861x	0.996 1	0.413	0.339~0.563
50% Prochloraz-copper salt suspension concentrate
40%吡唑萘菌胺戊唑醇悬浮剂	y=1.193+1.572x	0.945 7	0.759	0.667~0.913
40% Isopyrazam + tebuconazole suspension concentrate
17%唑醚·氟环唑悬浮剂	y=1.402+2.970x	0.986 4	0.371	0.292~0.570
17% Azoxystrobin epoxiconazole suspension concentrate
42%肟菌·戊唑醇悬浮剂	y=1.804+3.107x	0.968 4	0.581	0.530~0.656
42% Trifloxystrobin tebuconazole suspension concentrate
27.8%噻呋·苯醚甲悬浮剂	y=1.597+3.016x	0.991 0	0.530	0.484~0.593
27.8% Thifluzamide difenoconazole suspension concentrate
30%苯甲·吡唑酯悬浮剂	y=1.932+3.333x	0.968 2	0.263	0.213~0.319
30% Difenoconazole pyraclostrobin suspension concentrate
24%双胍·吡唑酯可湿性粉剂	y=1.929+2.555x	0.998 0	0.176	0.158~0.200
24% Guazatine pyraclostrobin wettable powder
27%春雷·溴菌腈可湿性粉剂	y=1.098+1.876x	0.965 2	0.889	0.783~1.063
27% Bromothalonil+kasugamycin wettable powder

杀菌剂 Fungicide	回归方程 Regression equation	R²	EC₅₀/(mg·L^-1)
杀菌剂 Fungicide	回归方程 Regression equation	R²	平均值Mean	95%CI
45%吡醚·甲硫灵悬浮剂	y=1.481+2.147x	0.999 5	0.690	0.635~0.751
45% Pyraclostrobin + thiophanate-methyl suspension concentrate
60%唑醚·戊唑醇水分散粒剂	y=1.196+3.261x	0.990 3	0.367	0.331~0.410
60% Azoxystrobin+tebuconazole water dispersible granules
50%咪鲜胺铜盐悬浮剂	y=1.594+3.861x	0.996 1	0.413	0.339~0.563
50% Prochloraz-copper salt suspension concentrate
40%吡唑萘菌胺戊唑醇悬浮剂	y=1.193+1.572x	0.945 7	0.759	0.667~0.913
40% Isopyrazam + tebuconazole suspension concentrate
17%唑醚·氟环唑悬浮剂	y=1.402+2.970x	0.986 4	0.371	0.292~0.570
17% Azoxystrobin epoxiconazole suspension concentrate
42%肟菌·戊唑醇悬浮剂	y=1.804+3.107x	0.968 4	0.581	0.530~0.656
42% Trifloxystrobin tebuconazole suspension concentrate
27.8%噻呋·苯醚甲悬浮剂	y=1.597+3.016x	0.991 0	0.530	0.484~0.593
27.8% Thifluzamide difenoconazole suspension concentrate
30%苯甲·吡唑酯悬浮剂	y=1.932+3.333x	0.968 2	0.263	0.213~0.319
30% Difenoconazole pyraclostrobin suspension concentrate
24%双胍·吡唑酯可湿性粉剂	y=1.929+2.555x	0.998 0	0.176	0.158~0.200
24% Guazatine pyraclostrobin wettable powder
27%春雷·溴菌腈可湿性粉剂	y=1.098+1.876x	0.965 2	0.889	0.783~1.063
27% Bromothalonil+kasugamycin wettable powder

杀菌剂 Fungicide	病情指数Disease index				防治效果Control efficacy/%
杀菌剂 Fungicide	药前 Before medication	第1次药后7 d 7 days after the 1^st medication	第2次药后7 d 7 days after the 2^nd medication	第3次药后7 d 7 days after the 3^rd medication	第1次药后7 d 7 days after the 1^st medication	第2次药后7 d 7 days after the 2^nd medication	第3次药后7 d 7 days after the 3^rd medication
45%吡醚·甲硫灵悬浮剂	7.18	5.71	5.41	5.33	58.71 bcd	70.21 c	81.24 cd
45% Pyraclostrobin + thiophanate- methyl suspension concentrate
60%唑醚·戊唑醇水分散粒剂	7.21	7.18	7.27	7.05	47.91 e	59.82 e	75.15 ef
60% Azoxystrobin+tebuconazole water dispersible granules
50%咪鲜胺铜盐悬浮剂	7.22	4.91	4.23	2.61	64.17 abc	76.65 b	90.83 a
50% Prochloraz-copper salt suspension concentrate
40%吡唑萘菌胺戊唑醇悬浮剂	7.29	6.39	6.19	6.43	53.64 cde	65.83 d	77.32 e
40% Isopyrazam + tebuconazole suspension concentrate
17%唑醚·氟环唑悬浮剂	7.23	6.83	6.39	5.39	50.53 de	64.72 d	80.95 d
17% Azoxystrobin epoxiconazole suspension concentrate
42%肟菌·戊唑醇悬浮剂	7.24	7.41	7.52	7.64	46.33 e	58.49 a	73.15 f
42% Trifloxystrobin tebuconazole suspension concentrate
27.8%噻呋·苯醚甲悬浮剂	7.35	6.01	5.36	4.63	56.37 bcde	70.41 c	83.68 c
27.8% Thifluzamide difenoconazole suspension concentrate
30%苯甲·吡唑酯悬浮剂	7.41	4.16	3.31	2.42	69.86 a	81.71 a	91.51 a
30% Difenoconazole pyraclostrobin suspension concentrate
24%双胍·吡唑酯可湿性粉剂	7.08	5.33	5.20	3.40	61.46 abc	71.30 c	88.00 b
24% Guazatine pyraclostrobin wettable powder
27%春雷·溴菌腈可湿性粉剂	7.28	4.68	4.24	5.25	66.14 ab	76.59 b	81.48 cd
27% Bromothalonil+kasugamycin wettable powder
CK	7.21	13.92	18.17	28.46	—	—	—

Pathogens identification of pepper anthracnose and fungicide screening

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