Fludioxonil， a phenylpyrrole fungicide， is effective to control many plant diseases through interfering high glycerol response pathway. In this study， sensitivities to fludioxonil of seventy\|eight isolates of Penicillium digitatum collected from Quzhou in Zhejiang province and Gannan in Jiangxi province were determined, based on tests of conidial germination and mycelial growth. The results demonstrated that the effective concentrations （EC50 values） against conidial germination and mycelial growth by 50% are ranged from 0.0285 μg·mL-1 to 0.11 μg·mL-1 and from 0.0019 μg·mL-1 to 0.04 μg·mL-1; averaged （0.0557±0.0041） μg·mL-1 and （0.0118±0.0015） μg·mL-1， respectively. Frequency distributions of EC50 values of 78 isolates growth were normally distributed， which suggested these two values could be used as the sensitivity baselines.