Abstract: MDCK\|Sus cell strain was adapted and screened out by reducing serum concentration in culture medium， the growth and metabolism status， abundance of avian influenza virus （AIV） receptor on cell membrane were detected and AIV replication in bioreactor scale was primarily applied. The results showed that the average specific growth rate of 0556 d-1 and maximum viable cell density of 242×106 cells·mL-1 could be achieved in MDCK\|Sus single\|cell suspension growth mode in serum free media after adaptation and screening. Normal abundance of AIV receptor， sialic acid\|α\|2，3\|gal， could be detected on MDCK\|Sus cells membrane. AIV\|H9 JS03 virus strain could be replicated with 8log2·25μL-1 of HA titer in MDCK\|Sus cells suspension cultured in bioreactor with the condition of MOI 005 and 1μg·mL-1 TPCK treated proteinase.

Key words: MDCK cells, Avian influenza virus, single\, cell suspension culture, AIV receptor