Abstract: With the purpose of fast detecting of Curtobacterium flaccumfaciens pv. flaccumfaciens in imported soybean， we established nested PCR with the reported primer CffFOR and the primer CffF11 based on the REP\|PCR sequenced results of C. flaccumfaciens pv. flaccumfaciens and testified its specificity and sensitivity. In specificity assays， only the samples of C. flaccumfaciens pv. flaccumfaciens were positive， while the other pathogens were negative. The detecting sensitivity of the bacterial suspension using nested PCR was 31×103 cfu·mL-1 which was 1 000 times higher than normal PCR. Moreover， it could detect the pathogen in the soybean sample with 05% infected rate within 1 h， which significantly shortened the testing time. One hundred imported samples in the lab were detected by nested PCR， the specific DNA band could be amplified only from one sample， and the sequence results showed that the soybean sample was indeed infected by C. flaccumfaciens pv. flaccumfaciens. The nested PCR established in this study has properties of strong specificity， high sensitivity， shorter testing time and accurate result， it can be used in detecting C. flaccumfaciens pv. flaccumfaciens for imported soybean.

Key words: Curtobacterium flaccumfaciens pv. flaccumfaciens, nested PCR, detection