Studies on isolation, identification of cold-resistant petroleum-degrading strain and its degradation capability

doi:10.3969/j.issn.1004-1524.2016.10.21

›› 2016, Vol. 28 ›› Issue (10): 1781-1789.DOI: 10.3969/j.issn.1004-1524.2016.10.21

• Environmental Science • Previous Articles Next Articles

Studies on isolation, identification of cold-resistant petroleum-degrading strain and its degradation capability

YANG Rui-qi¹, XUE Lin-gui^{1, *}, CHANG Si-jing², ZHANG Gao-sen², CHEN Tuo², LIU Guang-xiu²

1. College of Chemical and Biology Engineering, Lanzhou Jiaotong University, Key Laboratory of Extreme Environmental Microbial Resources and Engineering of Gansu Province, Lanzhou 730070, China;
2. Key Laboratory of Extreme Environmental Microbial Resources and Engineering of Gansu Province, Key laboratory of Desert and Desertification, Cold and Arid Regions Environmental and Engineering Research Institute, Chinese Academy of Sciences, Lanzhou 730000, China

Received:2015-12-23 Online:2016-10-15 Published:2016-11-20

Abstract

Abstract: We obtained a low-temperature resistant crude oil degrading strain known as YF28-1(8) from the soil samples in Qinghai-Tibet Plateau, Qilian mountain, whose degradation rate was (57.90±3.86)%. Besides, after a preliminary identification on the basis of investigation on its biochemical and biological characteristics and 16S rRNA homology analysis, we defined this strain as Rhodococcus erythropolis. In addition, we drew a conclusion about degradation capability by means of GC-MS, UV-spectrophotometry, gravimetry that YF28-1(8) was able to biodegrade all alkane constituents in different degrees and especially effective to linear saturated alkane. The initial optimum conditions of this strain included: pH value at 7.5, temperature at 30 ℃ and inoculation size of 800 μL and its quantity and degradation rate could reach maximum in only 8 days, meanwhile, surfactant named Tween 80 could only slightly improve its degradation rate.

Key words: low-temperature, crude oil degrading bacterial, GC-MS, degradation capability

CLC Number:

S476.1

YANG Rui-qi, XUE Lin-gui, CHANG Si-jing, ZHANG Gao-sen, CHEN Tuo, LIU Guang-xiu. Studies on isolation, identification of cold-resistant petroleum-degrading strain and its degradation capability[J]. , 2016, 28(10): 1781-1789.

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Studies on isolation, identification of cold-resistant petroleum-degrading strain and its degradation capability

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