Abstract: The purpose of the present study was to screen a high γaminobutyric acid （GABA） producing probiotics， which had the ability of biological antagonism and tolerance of acid and bile salt. The suspicious strains which producing GABA were analyzed qualitatively and quantitively by paper chromatography and high performance liquid chromatography and mass spectrometry （HPLCMS）. Combining the morphological， physiochemical properties with 16S rDNA sequencing analysis， a high GABAproducing bacterium was identified. Then the inhibition of this high GABAproducing strain against three pathogen bacteria from animal and the tolerance of acid and bile salt were performed. The results showed that the strain GA8 which isolated from pickle had high yield of GABA; it was cultured in GYP medium containing 1% Lglutamate at 37 ℃ for 48 h， the yield of GABA reached 3.50 g·L-1; it was identified as Lactobacillus plantarum. The bacteria liquid and supernatant of GA8 strain culture had some inhibitory effect on Salmonella pullorum， Escherichia coli and Staphylococcus aureus， and GA8 strain had no bacteriostasis ability. When the initial concentration of living bacteria was 108 cfu·mL-1， the living bacteria could reach more than 106 cfu·mL-1 after cultured on medium with pH 2.0 for 4 h and 103 cfu·mL-1 after cultured on medium contained bile salt 0.3% for 4 h. The results indicated that the strain GA8 had some antibacterial properties and acid resistance， but bile salt resistant ability was not prominent.

Key words: probiotics, γaminobutyric acid, bioantagonism, acid and bile salt tolerance