Effects of dietary supplementation with compound probiotics on production performance, intestinal antioxidant capacity and intestinal ammonia nitrogen levels in late-phase laying hens

doi:10.3969/j.issn.1004-1524.2023.02.04

Acta Agriculturae Zhejiangensis ›› 2023, Vol. 35 ›› Issue (2): 275-284.DOI: 10.3969/j.issn.1004-1524.2023.02.04

• Animal Science • Previous Articles Next Articles

Effects of dietary supplementation with compound probiotics on production performance, intestinal antioxidant capacity and intestinal ammonia nitrogen levels in late-phase laying hens

CHEN Xin¹(), CI Wenjia¹, LI Zhefeng², CAI Pingli³, ZHU Jianjin¹^,^*()

1. School of Food Science and Technology, Jiangnan University, Wuxi 214122, Jiangsu, China
2. Hangzhou King Technology Feed Co., Ltd., Hangzhou 311107, China
3. Poultry Technical Guidance Station, Tongshan District, Xuzhou, Xuzhou 221100, Jiangsu, China

Received:2021-11-19 Online:2023-02-25 Published:2023-03-14
Contact: ZHU Jianjin

Abstract

Abstract:

This study was aimed to investigate the effects of of dietary supplementation of Lactobacillus acidophilus and Bacillus subtilis on the performance and intestinal health of late-phase laying hens. A total of 2 160 Hy-Line brown hens (50-weeks-old) were randomly divided into three groups, the control group (NC group) was fed the basal diet and the experimental group was supplemented with 250 mg·kg^-1 (LD group) and 500 mg·kg^-1 (HD group) Lactobacillus acidophilus and Bacillus subtilis complexes on the basal diet. The results showed that compared with the NC group, the egg production rate increased by 2.62% and 2.20% in the LD and HD groups respectively and the average daily feed intake (ADFI) was significantly lower in the HD group (P<0.05) at the 49 d. Compared with the NC group, the activity of catalase (CAT) and glutathione peroxidase (GSH-Px) and total antioxidant capacity (T-AOC) in ileal tissue in the LD group were significantly higher than those in the NC group. Similarly, T-AOC, GSH-Px, CAT and SOD activities in cecum and ileum tissues were significantly increased (P<0.05) in the HD group. Significantly higher levels of acetic acid, n-valeric acid and isovaleric acid in cecum contents in the LD group (P<0.05), and significantly higher levels of acetic acid, propionic acid and n-butyric acid in the HD group (P<0.05) compared with the NC group. Ammonia nitrogen, urea content and relative activity of urease in the cecum contents were significantly lower (P<0.05) compared with the NC group. However, the levels of uric acid were higher (P<0.05) in the HD group compared with the NC group. The correlation between microbiome and ammonia metabolites showed that the relative abundance (Bacteroides, Clostridium, Ruminococcus, etc.) were significantly correlated with ammonia metabolites. In conclusion, dietary supplementation of Lactobacillus acidophilus and Bacillus subtilis can improve the antioxidant capacity of the intestine, increase the level of short-chain fatty acids in the intestinal contents and reduce the ammonia content in the intestinal contents for improving the performance of laying hens in the late egg-laying period.

Key words: probiotics, laying hen, production performance, gut health

CLC Number:

S831

CHEN Xin, CI Wenjia, LI Zhefeng, CAI Pingli, ZHU Jianjin. Effects of dietary supplementation with compound probiotics on production performance, intestinal antioxidant capacity and intestinal ammonia nitrogen levels in late-phase laying hens[J]. Acta Agriculturae Zhejiangensis, 2023, 35(2): 275-284.

Figures/Tables 9

Table 1 Composition and nutrient levels of basal diet

原料组成Crude material	含量Content/(g·kg^-1)	营养水平Nutritional level	总计Total
玉米Corn grain	620.00	代谢能Metabolic energy/(MJ·kg^-1)	11.02
豆粕Soybean meal	250.00	粗蛋白Crude protein/%	16.85
磷酸氢钙Dicalcium phosphate	40.00	钙Calcium/%	3.20
石粉Limestone	80.00	有效磷Available phosphorus/%	0.35
预混料Premix¹⁾	10.00	赖氨酸Lysine/%	0.85
合计Total	1 000.00	蛋氨酸+胱氨酸Methionine+Cystine/%	0.63

Fig.1 Effects of dietary supplementation with compound probiotics on laying performance of late-phase laying hens *represented a significant difference between NC group and LD group (P<0.05), and # represented a significant difference between HD group and NC group (P<0.05); No same letters on the bars indicated significant difference among groups (P<0.05). The same as below.

Table 2 Effects of dietary supplementation with compound probiotics on egg quality of late-phase laying hens

指标Parameters	NC	LD	HD
蛋壳强度	41.89±8.64	40.97±5.25	42.12±9.50
Eggshell strength/N
蛋白高度	5.87±1.51	5.96 ±1.59	6.72 ±0.69
Albumen height/mm
蛋黄颜色	6.97±1.05	6.99±0.70	6.63±0.32
Yolk color
哈夫单位	73.36±13.32	72.04±12.78	78.86±7.01
Haugh units
蛋形指数	1.30±0.04	1.32±0.04	1.31±0.05
Egg shape index

Fig.2 Effects of dietary supplementation with compound probiotics on the antioxidant capacity of cecum and ileum tissues in late-phase laying hens

Fig.3 Effects of dietary supplementation with compound probiotics on the histopathology of cecum in late-phase laying hens (H&E staining, ×200)

Fig.4 Effects of dietary supplementation with compound probiotics on the ammonia-related metabolites in the contents of the cecum and plasma in late-phase laying hens

Table 3 Effects of dietary supplementation with compound probiotics on the ammonia-related enzyme activity in the contents of the cecum in late-phase laying hens

项目Item	NC	LD	HD
脲酶活性 Urease activity/(U·mg^-1)	16.42 ±1.81	16.09 ±3.4	10.11 ±1.95
尿酸酶相对活性 The activity of uricase/%	100.42 ±2.04 a	91.83 ±1.52 ab	88.67 ±3.51 b
谷氨酸脱氢酶活性 GDH activity/(U·mg^-1)	31.65 ±3.37	39.81 ±5.64	35.19 ±3.16
谷氨酰胺合成酶活性 GS activity/(μmol·h^-1·	0.96 ±0.09	1.09 ±0.14	1.18 ±0.09
mg^-1 prot)

Fig.5 Effects of dietary supplementation with compound probiotics on the short-chain fatty acids in contents of cecum in late-phase laying hens

Fig.6 Correlation between ammonia nitrogen, urea and uric acid in cecum contents and relative abundance of cecum microbial in late-phase laying hens

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Effects of dietary supplementation with compound probiotics on production performance, intestinal antioxidant capacity and intestinal ammonia nitrogen levels in late-phase laying hens

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