LC-MS/MS-based study on effect of rice straw instead of partial corn silage on plasma metabolites of dairy cows

doi:10.3969/j.issn.1004-1524.2023.02.03

Acta Agriculturae Zhejiangensis ›› 2023, Vol. 35 ›› Issue (2): 266-274.DOI: 10.3969/j.issn.1004-1524.2023.02.03

• Animal Science • Previous Articles Next Articles

LC-MS/MS-based study on effect of rice straw instead of partial corn silage on plasma metabolites of dairy cows

LI Yanan(), YE Wenxing, ZHU Xiangde, CHEN Lin, XU Xiaofeng, ZHANG Lili()

College of Agriculture, Ningxia University, Yinchuan 750021, China

Received:2021-05-01 Online:2023-02-25 Published:2023-03-14
Contact: ZHANG Lili

Abstract

Abstract:

In order to investigate the effect of rice straw replacing part of corn silage on plasma metabolites of dairy cows, LC-MS/MS material separation and identification technology combined with multivariate statistical method was used in this paper to analyze the metabolites of rice straw before and after replacing corn silage. The results showed that after rice straw replaced part of corn silage, a total of 41 differential metabolites were detected in the plasma of the two groups. Twenty-two metabolites including epinephrine, β-hydroxybutyric acid, nicotinamide and cholic acid were up-regulated, and 19 metabolites such as glutathione, cortisol and corticosterone were down-regulated in the experimental group. It could be seen that replacing part of corn silage with rice straw enhanced the fat mobilization of dairy cows and reduced the ability to resist stress. The results provided a reference for the application of rice straw instead of corn silage in dairy cow diet, and provided a new idea for the development and utilization of rice straw resources.

Key words: rice straw, corn silage, dairy cow, plasma, metabolite

CLC Number:

S823.91

LI Yanan, YE Wenxing, ZHU Xiangde, CHEN Lin, XU Xiaofeng, ZHANG Lili. LC-MS/MS-based study on effect of rice straw instead of partial corn silage on plasma metabolites of dairy cows[J]. Acta Agriculturae Zhejiangensis, 2023, 35(2): 266-274.

Figures/Tables 8

References 33

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项目Items	对照组CS	试验组RSc
原料Ingredients
浓缩料Concentrate feed	13.84	13.84
压片玉米Flaked corn	22.50	22.50
甜菜颗粒Pelleted sugar beet pulp	2.00	2.00
啤酒糟Brewer’s grains	1.53	1.53
苜蓿青贮Alfalfa silage	4.12	4.12
苜蓿Alfalfa	10.32	10.32
全株玉米青贮Whole-plant corn silage	45.06	30.06
稻草Rice straw		15.00
小苏打Sodium bicarbonate	0.166	0.166
益康XP Yikang XP	0.464	0.464
合计Total	100.00	100.00
营养水平Nutrient levels¹⁾
粗蛋白质Crude protein	11.69	10.98
产奶净能Net energy/(MJ·kg^-1)	5.94	5.58
钙Ca	0.67	0.64
磷P	0.28	0.26
中性洗涤纤维Neutral detergent fiber	30.74	26.25

项目Items	对照组CS	试验组RSc
原料Ingredients
浓缩料Concentrate feed	13.84	13.84
压片玉米Flaked corn	22.50	22.50
甜菜颗粒Pelleted sugar beet pulp	2.00	2.00
啤酒糟Brewer’s grains	1.53	1.53
苜蓿青贮Alfalfa silage	4.12	4.12
苜蓿Alfalfa	10.32	10.32
全株玉米青贮Whole-plant corn silage	45.06	30.06
稻草Rice straw		15.00
小苏打Sodium bicarbonate	0.166	0.166
益康XP Yikang XP	0.464	0.464
合计Total	100.00	100.00
营养水平Nutrient levels¹⁾
粗蛋白质Crude protein	11.69	10.98
产奶净能Net energy/(MJ·kg^-1)	5.94	5.58
钙Ca	0.67	0.64
磷P	0.28	0.26
中性洗涤纤维Neutral detergent fiber	30.74	26.25

序号	代谢物	变量投影重要度	P值	差异倍数	趋势
No.	Metabolites	VIP	P-value	Fold change	Trend
1	氨基水杨酸Mesalamine	2.09	0	2.2	上调Up
2	4-羟基维甲酸4-Hydroxyretinoic Acid	1.91	0	2.47	上调Up
3	烟酰胺Nicotinamide	1.35	0	1.83	上调Up
4	苯乙烯Styrene	1.79	0.001	2.36	上调Up
5	前列腺素B₂ Prostaglandin B₂	2.07	0.001	2.63	上调Up
6	别胆酸Allocholic acid	1.69	0.001	3.12	上调Up
7	肾上腺素Epinephrine	1.62	0.001	1.54	上调Up
8	哌啶酸Pipecolinic Acid	1.52	0.008	2.51	上调Up
9	N-乙酰缬氨酸N-Acetyl valine	1.32	0.010	1.54	上调Up
10	肉碱Carnitine	1.28	0.013	1.89	上调Up
11	7-甲基鸟嘌呤7-Methylguanine	1.62	0.014	1.55	上调Up
12	四氢皮质酮Tetrahydro corticosterone	2.01	0	0.26	下调Down
13	4-苯基丁酸4-Phenylbutyric acid	1.92	0	0.39	下调Down
14	皮质酮Corticosterone	2.15	0	0.22	下调Down
15	L-胱硫醚L-Cystathionine	1.41	0	0.55	下调Down
16	L-鸟氨酸L-Ornithine	1.91	0	0.61	下调Down
17	棕榈油酸Palmitoleic acid	1.78	0.001	0.46	下调Down
18	磺乙酸Sulfoacetic acid	1.67	0.004	0.49	下调Down
19	21-脱氧皮质醇21-Deoxycortisol	1.72	0.006	0.56	下调Down
20	4-吡哆醇酸4-Pyridoxic acid	1.62	0.007	0.62	下调Down
21	胆甾酮Cholest-4-en-3-one	1.64	0.008	0.53	下调Down
22	癸酰肉碱Decanoyl carnitine	1.06	0.008	0.56	下调Down
23	二十碳五烯酸Eicosatetraenoic acid	1.12	0.010	0.63	下调Down
24	皮质醇Cortisol	1.4	0.011	0.45	下调Down
25	谷胱甘肽Glutathione	1.17	0.014	0.35	下调Down
26	L-羟脯氨酸L-Hydroxyproline	1.41	0.027	0.64	下调Down

序号	代谢物	变量投影重要度	P值	差异倍数	趋势
No.	Metabolites	VIP	P-value	Fold change	Trend
1	氨基水杨酸Mesalamine	2.09	0	2.2	上调Up
2	4-羟基维甲酸4-Hydroxyretinoic Acid	1.91	0	2.47	上调Up
3	烟酰胺Nicotinamide	1.35	0	1.83	上调Up
4	苯乙烯Styrene	1.79	0.001	2.36	上调Up
5	前列腺素B₂ Prostaglandin B₂	2.07	0.001	2.63	上调Up
6	别胆酸Allocholic acid	1.69	0.001	3.12	上调Up
7	肾上腺素Epinephrine	1.62	0.001	1.54	上调Up
8	哌啶酸Pipecolinic Acid	1.52	0.008	2.51	上调Up
9	N-乙酰缬氨酸N-Acetyl valine	1.32	0.010	1.54	上调Up
10	肉碱Carnitine	1.28	0.013	1.89	上调Up
11	7-甲基鸟嘌呤7-Methylguanine	1.62	0.014	1.55	上调Up
12	四氢皮质酮Tetrahydro corticosterone	2.01	0	0.26	下调Down
13	4-苯基丁酸4-Phenylbutyric acid	1.92	0	0.39	下调Down
14	皮质酮Corticosterone	2.15	0	0.22	下调Down
15	L-胱硫醚L-Cystathionine	1.41	0	0.55	下调Down
16	L-鸟氨酸L-Ornithine	1.91	0	0.61	下调Down
17	棕榈油酸Palmitoleic acid	1.78	0.001	0.46	下调Down
18	磺乙酸Sulfoacetic acid	1.67	0.004	0.49	下调Down
19	21-脱氧皮质醇21-Deoxycortisol	1.72	0.006	0.56	下调Down
20	4-吡哆醇酸4-Pyridoxic acid	1.62	0.007	0.62	下调Down
21	胆甾酮Cholest-4-en-3-one	1.64	0.008	0.53	下调Down
22	癸酰肉碱Decanoyl carnitine	1.06	0.008	0.56	下调Down
23	二十碳五烯酸Eicosatetraenoic acid	1.12	0.010	0.63	下调Down
24	皮质醇Cortisol	1.4	0.011	0.45	下调Down
25	谷胱甘肽Glutathione	1.17	0.014	0.35	下调Down
26	L-羟脯氨酸L-Hydroxyproline	1.41	0.027	0.64	下调Down

序号 No.	代谢物 Metabolites	变量投影重要度 VIP	P值 P-value	差异倍数 Fold Change	趋势 Trend
1	前列腺素D₃ Prostaglandin D₃	2.58	0	4.85	上调Up
2	2,4-二羟基苯甲酸2,4-Dihydroxybenzoic acid	1.90	0	3.76	上调Up
3	十四烷二酸Tetradecane dioic acid	2.22	0	1.77	上调Up
4	胆酸Cholic acid	2.28	0	2.66	上调Up
5	十二烷二酸Dodecanedioic acid	2.01	0	1.77	上调Up
6	β-羟基丁酸Beta hydroxybutyrate	1.25	0	1.68	上调Up
7	脱氧胆酸Deoxycholic Acid	1.83	0.003	4.15	上调Up
8	丁基苯甲酸Butylparaben	1.87	0.005	1.79	上调Up
9	肌肽Carnosine	1.59	0.008	2.38	上调Up
10	新喋呤Neopterin	1.70	0.014	3.33	上调Up
11	2,3-Dinor-TXB₂ 2,3-Dinor-TXB₂	1.45	0.015	1.53	上调Up
12	3b,7b-二羟基-5-雄甾烯-17-酮3b,7b-Dihydroxy-5-androsten-17-one	2.43	0	0.21	下调Down
13	12-羟基二十碳四烯酸12-Hete	1.56	0	0.63	下调Down
14	2,4,6-三甲基苯酚2,4,6-Trimethylphenol	2.67	0	0.31	下调Down
15	20-羟基二十碳四烯酸20-Hydroxy-(5Z,8Z,11Z,14Z)-eicosatetraenoic acid	2.43	0	0.32	下调Down

LC-MS/MS-based study on effect of rice straw instead of partial corn silage on plasma metabolites of dairy cows

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进样时间Time/min	A/%	B/%
0	98	2
1.5	98	2
12	0	100
14	0	100
14.1	98	2
17	98	2

进样时间Time/min	A/%	B/%
0	98	2
1.5	98	2
12	0	100
14	0	100
14.1	98	2
17	98	2