›› 2017, Vol. 29 ›› Issue (8): 1221-1227.DOI: 10.3969/j.issn.1004-1524.2017.08.01

• Crop Science •     Next Articles

Improving rice quality of ZH7954 by molecular marker-assisted selection

NIU Kaimeng1, 2, XU Junbo2, ZHONG Liang2, WANG Hua2, ZHU Ying2, *   

  1. 1. College of Chemistry and Life Science, Zhejiang Normal University, Jinhua 321004, China;
    2. State Key Laboratory Breeding Base for Zhejiang Sustainable Pest and Disease Control, Institute of Virology and Biotechnology, Zhejiang Academy of Agricultural Sciences, Hangzhou 310021, China
  • Received:2017-03-09 Online:2017-08-20 Published:2017-09-06

Abstract: Zhehui7954 (ZH7954) is an elite restorer line with high combining ability for three-line indica hybrid rice production. However, the amylose content of ZH7954 was high (25%), which resulted in poor eating and cooking quality. Improvement of rice quality of ZH7954 was hard to achieve by using traditional breeding strategies. In this study, a codominant molecular marker named PCR-AccI was developed based on the G/T SNP at the first intron splicing site of rice Wx gene which mainly determines the amylose biosynthesis in rice endosperm. Here, an elite parental line 93-11 with intermediate AC (17%) and T-type Wx(called Wxb) were used as donors. Two lines L2 and L9 were obtained with intermediate AC but similar phenotype to ZH7954 by backcross and molecular marker-assisted selection. Both of them had better eating and cooking quality than receptor line ZH7954, while the appearance quality, such as grain shape, grain weight and milled rice rate were almost identical to ZH7954. These results suggested that the quality of ZH7954 was successfully improved through combining the traditional breeding and marker assisted selection. Data from SNP-typing showed that less than 7% of genome from 93-11 was integrated to ZH7954 in both L2 and L9. The combining ability would be further tested and such intermediate lines might be the valuable materials to generate elite restorer line with high yield and good quality in the future.

Key words: ZH7954, amylose content, Wx gene, marker-assisted selection, SNP

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