Function of DnaJ-like proteins in infection of Turnip mosaic virus onto Nicotiana benthamiana

doi:10.3969/j.issn.1004-1524.2018.12.10

›› 2018, Vol. 30 ›› Issue (12): 2056-2064.DOI: 10.3969/j.issn.1004-1524.2018.12.10

• Plant Protection • Previous Articles Next Articles

Function of DnaJ-like proteins in infection of Turnip mosaic virus onto Nicotiana benthamiana

SUN Chaochen^1,2, YAN Fei², CHEN Jianping^2,*

1. College of Plant Protection, Yunnan Agricultural University, Kunming 650201, China;
2. State Key Laboratory Breeding Base of Zhejiang Sustainable Pest and Disease Control, Key Laboratory of Biotechnology in Plant Protection of Ministry of Agriculture and Rural Affairs, Key Laboratory of Biotechnology in Plant Protection of Zhejiang Province, Institute of Virology and Biotechnology, Zhejiang Academy of Agricultural Sciences, Hangzhou 310021, China

Received:2018-04-23 Online:2018-12-25 Published:2018-12-28

Abstract

Abstract: Heat shock protein 40 (HSP40) contains a conserved J domain, also named DnaJ protein. Its homologous protein is called DnaJ-like protein. Current research shows that HSP40 family responds to the infection of multiple viruses, but the ways in their respective functions are different. Turnip mosaic virus (TuMV) is an important vegetable virus that causes serious economic losses to vegetable production. At present, it is unclear whether the DnaJ-like protein responds to the infection of TuMV and whether DnaJ-like protein play a role in TuMV infection. In this study, 16 genes belonging to DnaJ protein class A subfamily and B subfamily were cloned from Nicotiana benthamiana. The qRT-PCR analysis showed that DnaJ-like protein family genes were up-regulated in TuMV-infected N. benthamiana. Silencing the genes NbJ1 and NbJ5, the typical gene of subfamily A and B, respectively, caused the reduced TuMV accumulation. The results showed that DnaJ-like protein might play a role in TuMV infecting, which provided a new way to understand the pathogenic mechanism of TuMV.

Key words: HSP40, turnip mosaic virus, homology analysis

CLC Number:

S432.1

SUN Chaochen, YAN Fei, CHEN Jianping. Function of DnaJ-like proteins in infection of Turnip mosaic virus onto Nicotiana benthamiana[J]. , 2018, 30(12): 2056-2064.

References

[1] DE C A, KOHIYAMA M, RICHARME G.A novel function of Escherichia coli chaperone DnaJ. protein-disulfide isomerase[J]. Journal of Biological Chemistry, 1995, 270(39):22669-22672.
[2] RAJAN VB, D'SILVA P. Arabidopsis thaliana J-class heat shock proteins: cellular stress sensors[J]. Functional & Integrative Genomics, 2009, 9(4):433.
[3] LIU J Z, WHITHAM S A.Overexpression of a soybean nuclear localized type-Ⅲ DnaJ domain-containing HSP40 reveals its roles in cell death and disease resistance[J]. Plant Journal, 2013, 74(1):110-121.
[4] SEDBROOK J C, CHEN R, MASSON P H.ARG1 (Altered response to gravity) encodes a DnaJ-like protein that potentially interacts with the cytoskeleton[J]. Proceedings of the National Academy of Sciences of the United States of America, 1999, 96(3):1140-1145.
[5] REN C E, ZHU X, LI J, et al.Microarray analysis on gene regulation by estrogen, progesterone and tamoxifen in human endometrial stromal cells[J]. International Journal of Molecular Sciences, 2015, 16(3):5864-5885.
[6] ACUN T, DOBERSTEIN N, HABERMANN J K, et al.HLJ1 (DNAJB4) gene is a novel biomarker candidate in breast cancer[J]. Omics, 2017, 21(5):257-265.
[7] CRAIG E A, GAMBILL B D, NELSON R J.Heat shock proteins: molecular chaperones of protein biogenesis[J]. Microbiological Reviews, 1993, 57(2):402-414.
[8] SUSIN M F, BALDINI R L, GUEIROS-FILHO F, et al.GroES/GroEL and DnaK/DnaJ have distinct roles in stress responses and during cell cycle progression in Caulobacter crescentus[J]. Journal of Bacteriology, 2006, 188(23):8044.
[9] WANG G, CAI G, KONG F, et al.Overexpression of tomato chloroplast-targeted DnaJ protein enhances tolerance to drought stress and resistance to Pseudomonas solanacearum in transgenic tobacco[J]. Plant Physiology and Biochemistry, 2014, 82(3):95-104.
[10] KELLEY W L.The J-domain family and the recruitment of chaperone power[J]. Trends in Biochemical Sciences, 1998, 23(6):222-227.
[11] SOHN S Y, KIM S B, KIM J, et al.Negative regulation of hepatitis B virus replication by cellular Hsp40/DnaJ proteins through destabilization of viral core and X proteins[J]. Journal of General Virology, 2006, 87(7):1883-1891.
[12] WANG Y L, HUANG Y R, CHONG K M, et al.DnaJ homolog Hdj2 facilitates Japanese encephalitis virus replication[J]. Virology Journal, 2011, 8(1):1-7.
[13] TOMITA Y, MIZUNO T, DÍEZ J, et al. Mutation of host DnaJ homolog inhibits brome mosaic virus negative-strand RNA synthesis[J]. Journal of Virology, 2003, 77(5):2990-2997.
[14] SHIMIZU T, YOSHII A, SAKURAI K, et al.Identification of a novel tobacco DnaJ-like protein that interacts with the movement protein of tobacco mosaic virus[J]. Archives of Virology, 2009, 154(6):959-967.
[15] LU L, DU Z M, WANG P, et al.Pc4, a putative movement protein of Rice stripe virus, interacts with a type I DnaJ protein and a small Hsp of rice[J]. Virus Genes, 2009, 38(2):320-327.
[16] BARGEN S V, SALCHERT K, PAAPE M, et al.Interactions between the tomato spotted wilt virus movement protein and plant proteins showing homologies to myosin, kinesin and DnaJ-like chaperones[J]. Plant Physiology & Biochemistry, 2001, 39(12):1083-1093.
[17] HOFIUS D, MAIER A T, DIETRICH C, et al.Capsid protein-mediated recruitment of host DnaJ-like proteins is required for potato virus Y infection in tobacco plants[J]. Journal of Virology, 2007, 81(21):11870.
[18] CHO S Y, CHO W K, SOHN S H, et al.Interaction of the host protein NbDnaJ with Potato virus X, minus-strand stem-loop 1 RNA and capsid protein affects viral replication and movement[J]. Biochemical & Biophysical Research Communications, 2012, 417(1):451-456.
[19] SHUKLA D D, WARD C W, Brunt A A.The Potyviridae[M]. Wallingford: CAB International. 1994.
[20] WEINMANN W, BOHNERT M.Lethal monointoxication by overdosage of MDEA[J]. Forensic Science International, 1998, 91(2):91-101.
[21] 陈集双, 张耀洲, 徐平, 等. 甘蓝型油菜芜菁花叶病毒分离物TuMVH_1的生物学研究[J]. 中国油料作物学报, 1994 (1): 24-28.
CHEN J S, ZHANG Y Z, XU P, et al.Biological study of Brassica napus isolates TuMVH_1[J]. Chinese Journal of Oil Crops, 1994 (1): 24-28. (in Chinese)
[22] 陈瑞泰, 朱贤朝, 王智发, 等. 全国16个主产烟省(区)烟草侵染性病害调研报告[J]. 中国烟草科学, 1997 (18):1-7.
CHEN R T, ZHU X C, WANG Z F, et al.Report on tobacco infective diseases in 16 major tobacco producing provinces (regions)[J]. China Tobacco Science, 1997 (18):1-7. (in Chinese)
[23] CHO J D, CHOI H S, KIM J S, et al.Ultrastructural aspects of mixed infections with Turnip mosaic virus (TuMV-ACl8 and-C5) and Ribgrass mosaic virus (RMV-CA1) in oriental cabbage[J]. Plant Pathology Journal, 2002, 18:192-198.
[24] CHUNG P Y J, BEYENS G, GUAÑABENS N, et al. Founder effect in different european countries for the recurrent P392L SQSTM1 mutation in paget's disease of bone[J]. Calcif Tissue Int, 2008, 83(1):34-42.
[25] DU Y, ZHAO J, CHEN T, et al.Type I J-domain NbMIP1 proteins are required for both Tobacco mosaic virus infection and plant innate immunity[J]. Plos Pathogens, 2013, 9(10):e1003659.

Function of DnaJ-like proteins in infection of Turnip mosaic virus onto Nicotiana benthamiana

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