Isolation and identification of Elizabethkingia meningoseptica from Chinese spiny frog (Quasipaa spinosa) and pathological lesions of its infection

doi:10.3969/j.issn.1004-1524.2018.03.03

›› 2018, Vol. 30 ›› Issue (3): 371-377.DOI: 10.3969/j.issn.1004-1524.2018.03.03

• Animal Science • Previous Articles Next Articles

Isolation and identification of Elizabethkingia meningoseptica from Chinese spiny frog (Quasipaa spinosa) and pathological lesions of its infection

LEI Xueping^a, GENG Yi^a,*, YU Zehui^a, ZHENG Liping^a, CAO Shiqi^a, HUANG Xiaoli^b, CHEN Defang^b, OUYANG Ping^a, LIU Kairui^a

a.College of Veterinary Medicine Sichuan Agricultural University, Chengdu 611134, China;
b.Department of Aquaculture, Sichuan Agricultural University, Chengdu 611134, China

Received:2017-11-27 Online:2018-03-20 Published:2018-03-21

Abstract

Abstract: In April 2016, a serious infectious disease characterized by cataract, cutaneous ulcer and neurological symptoms emerged in a Quasipaa spinosa farm in Ya'an, Sichuan Province. In order to explore the etiology, the pathogen isolation, artificial infection, physical and chemical characteristics of isolated bacteria and sequence analysis of 16S rDNA gene were analyzed. The results showed that a Gram-negative, short rod-shaped bacillus (CM160701) was isolated from the diseased frogs. Healthy Chinese spiny frogs injected the isolate intraperitoneally exhibited the similar clinical signs, gross and histopathological lesions to the naturally infected frogs, and the LD₅₀ value of the isolate was 1.19×10⁶ cfu per frog. The isolated strain was identified to be Elizabethkingia meningoseptica according to physicochemical properties and the 16S rDNA gene sequence analysis. The strain was sensitive to florfenicol, but resistant to enrofloxacin, amoxicillin, doxycycline and so on. Histopathological observation found that the E. meningoseptica infection in Quasipaa spinosa could cause pathological damages to multiple organs which showed degeneration, necrosis and inflammation significantly, especially in the brain, liver, spleen, heart and kidney. This study confirmed E. meningoseptica was the pathogen of the epidemic, and the E. meningoseptica infection could cause Quasipaa spinosa multi-system injuries and lead to death.

Key words: Quasipaa spinosa, Elizabethkingia meningoseptica, biological characteristics, pathological lesions

CLC Number:

S947.2

LEI Xueping, GENG Yi, YU Zehui, ZHENG Liping, CAO Shiqi, HUANG Xiaoli, CHEN Defang, OUYANG Ping, LIU Kairui. Isolation and identification of Elizabethkingia meningoseptica from Chinese spiny frog (Quasipaa spinosa) and pathological lesions of its infection[J]. , 2018, 30(3): 371-377.

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Isolation and identification of Elizabethkingia meningoseptica from Chinese spiny frog (Quasipaa spinosa) and pathological lesions of its infection

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