Soil microbial community structure, labile organic carbon and nitrogen and enzyme activities in paddy field and upland affected by long-term fertilization systems

doi:10.3969/j.issn.1004-1524.2018.05.20

›› 2018, Vol. 30 ›› Issue (5): 817-824.DOI: 10.3969/j.issn.1004-1524.2018.05.20

• Environmental Science • Previous Articles Next Articles

Soil microbial community structure, labile organic carbon and nitrogen and enzyme activities in paddy field and upland affected by long-term fertilization systems

WANG Guiyue, SU Ting, HAN Hailiang, TAN Heping, BAO Fei, ZHAO Fucheng^*

Dongyang Institute of Maize Research, Zhejiang Academy of Agricultural Sciences, Dongyang 322100, China

Received:2017-11-01 Online:2018-05-20 Published:2018-05-23

Abstract

Abstract: To investigate the effects of long-term fertilization systems on soil microbial community structure, labile organic carbon and nitrogen, and enzyme activities in yellow sand paddy field and upland, a field experiment was conducted at the experimental station of Dongyang Institute of Maize Research, Zhejiang, China in 2009. The experiment consisted of six treatments with three replicates and was arranged in a completely randomized design: PCK, no fertilization in paddy field; PCF, conventional fertilization in paddy field; PSTF, formulated fertilization by soil testing in paddy field; PSTF+OF, formulated fertilization by soil testing with organic manure in paddy field; DCF, conventional fertilization on upland; DSTF+OF, formulated fertilization by soil testing with organic manure on upland. Soil nutrients, enzyme activities, microbial biomass and community structure were determined in 2015. It was shown that compared with no fertilization in paddy field (PCK), all fertilization treatments significantly (P<0.05) increased soil available phosphorus and potassium contents, and decreased soil pH value. All treatments had no significant effects on quantity of soil culturable bacteria and actinomycetes, yet PSTF+OF significantly (P<0.05) increased the quantity of soil culturable fungi. Formulated fertilization by soil testing with organic manure (PSTF+OF and DSTF+OF) significantly (P<0.05) increased soil microbial carbon and nitrogen contents both in paddy field and upland. All treatments had no significant effect on soil cellulose activity, while formulated fertilization by soil testing with organic manure (PSTF+OF and DSTF+OF) significantly (P<0.05) increased soil dehydrogenase and catalase activities. In general, application of chemical fertilizers with organic manure could improve soil fertility.

Key words: fertilization, soil fertility, long-term experiment

CLC Number:

S147.2

WANG Guiyue, SU Ting, HAN Hailiang, TAN Heping, BAO Fei, ZHAO Fucheng. Soil microbial community structure, labile organic carbon and nitrogen and enzyme activities in paddy field and upland affected by long-term fertilization systems[J]. , 2018, 30(5): 817-824.

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Soil microbial community structure, labile organic carbon and nitrogen and enzyme activities in paddy field and upland affected by long-term fertilization systems

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