Characterization of Salmonella isolates recovered from processing chicken, retail chicken and chicken processing environment in Zhejiang and Shanghai

doi:10.3969/j.issn.1004-1524.2019.07.17

›› 2019, Vol. 31 ›› Issue (7): 1161-1169.DOI: 10.3969/j.issn.1004-1524.2019.07.17

• Food Science • Previous Articles Next Articles

Characterization of Salmonella isolates recovered from processing chicken, retail chicken and chicken processing environment in Zhejiang and Shanghai

CAO Chenyang¹, ZHANG Yi¹, ZHANG Qian¹, XIAO Yingping², ZHAO Ruiting¹, LIU Chenxing³, YANG Hua², ZHAO Min⁴, YANG Baowei^1,*

1. College of Food Science and Engineering, Northwest A&F University, Yangling 712100, China;
2. Institute of Quality and Standard for Agro-products, Zhejiang Academy of Agricultural Sciences, Hangzhou 310021, China;
3. College of Animal Science, Zhejiang University, Hangzhou 310058, China;
4. Xi'an Wanlong Pharmaceutical Co.,Ltd, Yangling 712100, China;

Received:2018-11-21 Online:2019-07-25 Published:2019-08-07

Abstract

Abstract: One hundred and two Salmonella isolates recovered from processing chickens, retail chickens and environment of chicken processing slaughterhouses that collected in Zhejiang and Shanghai during July to October in 2016 were characterized to determine their prevalence, serotype and antimicrobial susceptibility. Serotype of Salmonella was determined via slide agglutination method using Salmonella hyperimmune sera, the antimicrobial susceptibility was tested by disk diffusion method that recommended by the clinical laboratory standard institute (CLSI). Among 204 samples, 85 samples were tested positive to Salmonella. 32 samples collected from processing chickens were positive to Salmonella, the highest rate of Salmonella positive samples was identified in the cold storage section with the ratio of 70.00%. A total of 15 serotypes were identified among the 102 Salmonella isolates. The most common five serotypes were S.Derby (23.53%), S. Agona (17.65%), S. Indiana (12.75%), S. Typhimurium (11.76%) and S. Dabou (7.84%). S. Derby was tested most prevalent in processing chickens while S. Agona was the most commonly found in retail chickens. All isolates were sensitive to amoxicillin/clavulanate and cefoxitin. Seventy five (73.53%) were resistant to tetracycline, followed by to chloramphenicol (49.02%), ampicillin (41.18%), nalidixic acid (38.24%), sulfamethoxazole/trimethoprim (37.25%), streptomycin (28.43%), ciprofloxacin (23.53%), ceftriaxone (22.55%), cefoperazone (22.55%), kanamycin (20.59%), gentamycin (19.61%) and amikacin (17.65%). Salmonella isolates in retail chickens showed significantly lower resistance to most tested antibiotics than those from processing chickens and slaughterhouse environment. Among 102 Salmonella isolates, 97 (95.10%) were resistant to at least one antibiotic, and 17 (16.67%) to 10 or more than 10 antimicrobials. Among all sampling places, Salmonella positive samples were investigated most prevalent in Shaoxing (78.95%), where the rate of Salmonella positive samples was significantly higher than those in Huzhou and Shanghai. The rates of Salmonella positive samples were found significantly different (P<0.01) in different sampling months, and it was found most prevalent (80.00%) in September. In the whole chicken production and consumption chain, the positive rate, serotype, antibiotic resistance rate, and antibiotic resistance phenotype of Salmonella isolates showed significant changes. Salmonella was not only derived from the chickens carrying bacteria in the batch, but also in various links and carried by the personnel also joined, resulting in the status of the terminal products. Prevalence of Salmonella in processing and retail chickens in Zhejiang and Shanghai was common. Chickens in processing, especially in refrigeration stage were more easily contaminated by Salmonella. The serotype of Salmonella isolates was diverse, and these isolates were resistant to multi-antibiotics.

Key words: Salmonella, serotype, antimicrobial susceptibility

CLC Number:

S85
TS251.55

CAO Chenyang, ZHANG Yi, ZHANG Qian, XIAO Yingping, ZHAO Ruiting, LIU Chenxing, YANG Hua, ZHAO Min, YANG Baowei. Characterization of Salmonella isolates recovered from processing chicken, retail chicken and chicken processing environment in Zhejiang and Shanghai[J]. , 2019, 31(7): 1161-1169.

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Characterization of Salmonella isolates recovered from processing chicken, retail chicken and chicken processing environment in Zhejiang and Shanghai

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