Optimization of fermentation techniques on yield of exopolysaccharide produced by Lactobacillus pentosae YY112

doi:10.3969/j.issn.1004-1524.2020.02.17

›› 2020, Vol. 32 ›› Issue (2): 327-336.DOI: 10.3969/j.issn.1004-1524.2020.02.17

• Food Science • Previous Articles Next Articles

Optimization of fermentation techniques on yield of exopolysaccharide produced by Lactobacillus pentosae YY112

WANG Mingzhe^1,2, YANG Ying², TANG Weimin², LIU Zhe², SUN Peilong¹, LU Shengmin^2,*

1.Ocean College, Zhejiang University of Technology, Hangzhou 310014,China;
2. Institute of Food Sciences, Zhejiang Academy of Agricultural Sciences, Hangzhou 310021, China

Received:2019-08-15 Online:2020-02-25 Published:2020-03-13

Abstract

Abstract: In order to improve the yield of exopolysaccharide produced by Lactobacillus pentosae YY112, the nutritional conditions and fermentation technology of the strain were optimized by single factor test, orthogonal test and Box-Behnken response surface design. The results showed that: within the scope of this study, MRS medium was the appropriate basic one, using 3% (m/V) sucrose was the appropriate carbon source, 1% (m/V) soybean peptone was the appropriate nitrogen source and 2% (m/V) yeast powder could increase polysaccharide yield of the strain. The initial pH value of the medium was 6.0, and the inoculation amount of the strain was 4% (V/V). The optimum fermentation temperature was 29.5 ℃, and the best fermentation time was 24.5 h. Under the optimized culture conditions, the yield of exopolysaccharide reached (380.97±0.45) mg·L^-1, which was basically consistent with the predicted value of response surface analysis, an increase of 46.52% compared with that before optimization.

Key words: lactic acid bacteria, exopolysaccharide, nutritional condition, technological condition, optimization

CLC Number:

TS201.3

WANG Mingzhe, YANG Ying, TANG Weimin, LIU Zhe, SUN Peilong, LU Shengmin. Optimization of fermentation techniques on yield of exopolysaccharide produced by Lactobacillus pentosae YY112[J]. , 2020, 32(2): 327-336.

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Optimization of fermentation techniques on yield of exopolysaccharide produced by Lactobacillus pentosae YY112

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