Cadmium tolerance mechanism of Solanum nigrum L. based on components and enzyme activity changes in apoplast

doi:10.3969/j.issn.1004-1524.2020.05.09

›› 2020, Vol. 32 ›› Issue (5): 816-823.DOI: 10.3969/j.issn.1004-1524.2020.05.09

• Horticultural Science • Previous Articles Next Articles

Cadmium tolerance mechanism of Solanum nigrum L. based on components and enzyme activity changes in apoplast

WANG Xiaoming^1,2, CHEN Ailing¹, DONG Lu¹, SHI Yujun¹, ZHENG Liuchang¹, ZHAO Lianhui¹, ZHANG Ruiqi¹, ZHANG Fenqin^1,2,*

1. College of Life Science and Engineering, Hexi University, Zhangye 734000, China;
2.Key Laboratory of Hexi Corridor Resources Utilization of Gansu, Zhangye 734000, China

Received:2019-11-28 Online:2020-05-25 Published:2020-05-29

Abstract

Abstract: To investigate the cadmium tolerance mechanism of Solanum nigrum L., a substrate culture experiment was conducted to study the effects of 50 μmol·L^-1 Cd (CdCl₂·2.5H₂O) treatment on the contents of pectin and lignin, the activities of polygalacturonase (PG), cellulase (Cx), NADH-peroxidase (NADH-POD), guaiacol peroxidase (GPOD) and coniferylalcohol peroxidase (CAPX) in cell wall, and the content of hydrogen peroxide (H₂O₂) and the activities of superoxide dismutase (SOD), catalase (CAT), ascorbic acid oxidase (APX) in apoplast sap. The results showed that after 50 μmol·L^-1 Cd treatment for 120 days, compared with the control, the length of shoot and root were inhibited, and the biomass of root was decreased (P<0.05), but the leaf area, chlorophyll content (SPAD), net photosynthetic rate (P_n) and intercellular CO₂ concentration (C_i) showed no significant difference (P>0.05). In the cell wall of leaves, the activity of NADH-POD, GPOD and CAPX and the pectin content were all significantly increased (P<0.05), the PG activity was decreased (P<0.05), and Cx activity and lignin content showed no significant difference (P>0.05). In the apoplast sap of leaves, H₂O₂ content increased significantly (P<0.05), and the activity and isozyme band of GPOD and SOD weren't significantly influenced (P>0.05), but the activities of APX and CAT increased (P<0.05) with more isozyme band number and enhanced isozyme band, respectively. In conclusion, the pectin, H₂O₂-producing NADH-POD and H₂O₂-consuming GPOD in cell wall, and H₂O₂-consuming APX and CAT in apoplast sap might play major roles in Cd tolerance of Solanum nigrum L.

Key words: Solanum nigrum L., apoplast, enzymes activity, cadmium tolerance

CLC Number:

S332.9

WANG Xiaoming, CHEN Ailing, DONG Lu, SHI Yujun, ZHENG Liuchang, ZHAO Lianhui, ZHANG Ruiqi, ZHANG Fenqin. Cadmium tolerance mechanism of Solanum nigrum L. based on components and enzyme activity changes in apoplast[J]. , 2020, 32(5): 816-823.

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Cadmium tolerance mechanism of Solanum nigrum L. based on components and enzyme activity changes in apoplast

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