Treatment of diseased broilers with Shigella bacteriophage ΦDS8 and the effect on their intestinal flora

doi:10.3969/j.issn.1004-1524.2022.07.05

Acta Agriculturae Zhejiangensis ›› 2022, Vol. 34 ›› Issue (7): 1386-1395.DOI: 10.3969/j.issn.1004-1524.2022.07.05

• Animal Science • Previous Articles Next Articles

Treatment of diseased broilers with Shigella bacteriophage ΦDS8 and the effect on their intestinal flora

CHEN Shiyu¹^,²(), XU Meiyu¹^,², DENG Zhengyu¹^,², WANG Feng¹^,², ZHANG Qilin¹^,², DENG Xianyu¹^,², LIN Lianbing¹^,²^,^*()

1. Faculty of Life Science and Technology, Kunming University of Science and Technology, Kunming 650500, China
2. Engineering Research Center for Replacement Technology of Feed Antibiotics of Yunnan College, Kunming 650500, China

Received:2021-04-22 Online:2022-07-25 Published:2022-07-26
Contact: LIN Lianbing

Abstract

Abstract:

Shigella is a common enteric pathogenic bacterium, and the bacterial disease it causes is one of the zoonotic diseases that endanger public health safety. With the misuse of antibiotics, the problem of Shigella resistance is difficult to control and new approaches are urgently needed to solve it. The use of bacteriophages to kill drug-resistant bacteria has received increasing attention. In this study, the Shigella infection model of broiler chickens was established. The broiler chickens were treated with phage fluid 2 h before and after infection. The broilers fed with Shigella for 2 h all had diarrhea, with the highest Shigella content in feces of 1×10⁷ CFU·g^-1, a large decrease in the content of dominant flora and a rise in the proportion of harmful flora. After phage therapy, the fecal phage content of broilers increased, Shigella content decreased, the fecal traits and intestinal flora returned to the normal, and the broiler survival rate was 100%. The survival rate of broiler chickens without phage treatment was 60%, and anatomical observation revealed obvious organ lesions. Bacteriophage ΦDS8 has shown promising efficacy in the treatment of Shigella strain infection, and it has good application value and prospect as an alternative product to antibiotics.

Key words: Shigella, bacteriophage, broiler, becal microflora

CLC Number:

S816.7

CHEN Shiyu, XU Meiyu, DENG Zhengyu, WANG Feng, ZHANG Qilin, DENG Xianyu, LIN Lianbing. Treatment of diseased broilers with Shigella bacteriophage ΦDS8 and the effect on their intestinal flora[J]. Acta Agriculturae Zhejiangensis, 2022, 34(7): 1386-1395.

Figures/Tables 10

References 34

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分组 Group	动物数量 Animal number	每只灌喂细菌量 Amount of bacteria per single animal/CFU	每只灌喂噬菌体量 Bacteriophage volume per single animal/PFU	灌喂噬菌体治疗时间点 Time point of phage therapy/h
阴性对照组Negative control	15	—	—	—
阳性对照组Positive control group	15	1×10⁸	—	—
噬菌体治疗组Therapeutic group	15	1×10⁸	1×10⁸	4
噬菌体预防组Prophylactic group	15	1×10⁸	1×10⁸	0
噬菌体治疗对照组Therapeutic control	15	—	1×10⁸	4
噬菌体预防对照组Prophylactic control	15	—	1×10⁸	0

分组 Group	动物数量 Animal number	每只灌喂细菌量 Amount of bacteria per single animal/CFU	每只灌喂噬菌体量 Bacteriophage volume per single animal/PFU	灌喂噬菌体治疗时间点 Time point of phage therapy/h
阴性对照组Negative control	15	—	—	—
阳性对照组Positive control group	15	1×10⁸	—	—
噬菌体治疗组Therapeutic group	15	1×10⁸	1×10⁸	4
噬菌体预防组Prophylactic group	15	1×10⁸	1×10⁸	0
噬菌体治疗对照组Therapeutic control	15	—	1×10⁸	4
噬菌体预防对照组Prophylactic control	15	—	1×10⁸	0

分组Group	0 h	2 h	4 h	6 h	12 h	24 h	48 h
阴性对照组Negative control	-	-	-	-	-	-	-
阳性对照组Positive control	-	+	+	++	+++	+++	++
噬菌体治疗组Therapeutic group	-	+	++	++	+	-	-
噬菌体预防组Prophylactic group	-	+	++	++	+	-	-
噬菌体治疗对照组Therapeutic control	-	-	-	-	-	-	-
噬菌体预防对照组Prophylactic control	-	-	-	-	-	-	-

分组Group	0 h	2 h	4 h	6 h	12 h	24 h	48 h
阴性对照组Negative control	-	-	-	-	-	-	-
阳性对照组Positive control	-	+	+	++	+++	+++	++
噬菌体治疗组Therapeutic group	-	+	++	++	+	-	-
噬菌体预防组Prophylactic group	-	+	++	++	+	-	-
噬菌体治疗对照组Therapeutic control	-	-	-	-	-	-	-
噬菌体预防对照组Prophylactic control	-	-	-	-	-	-	-

分组 Group	死亡数量 Number of deaths	存活率 Survival rate/%
阴性对照组Negative control	0	100
阳性对照组Positive control	6	60
噬菌体治疗组Therapeutic group	0	100
噬菌体预防组Prophylactic group	1	93
噬菌体治疗对照组Therapeutic control	0	100
噬菌体预防对照组Prophylactic control	0	100

Treatment of diseased broilers with Shigella bacteriophage ΦDS8 and the effect on their intestinal flora

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Figures/Tables 10

References 34

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样品 Sample	Ace指数 Ace	Chao1指数 Chao1	辛普森指数 Simpson	香农指数 Shannon	覆盖率 Coverage
0-A	268.734	272.7	0.313 2	2.627 4	0.983 6
0-B	312.564	286.2	0.091 8	3.568 5	0.966 8
2-A	229.834	236.4	0.113 0	3.157 7	0.984 8
2-B	282.828	269.3	0.084 4	3.404 6	0.982 3
12-A	222.394	169.1	0.08 23	3.524 2	0.990 7
12-B	207.277	217.3	0.353 1	2.121 1	0.976 8
48-A	224.704	235.7	0.536 9	1.779 6	0.998 2
48-B	207.677	299.1	0.111 0	3.074 0	0.991 7

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