Histological study on the effect of high temperature stress on liver tissue in Larimichthys polyactis

doi:10.3969/j.issn.1004-1524.20230230

Acta Agriculturae Zhejiangensis ›› 2024, Vol. 36 ›› Issue (1): 58-66.DOI: 10.3969/j.issn.1004-1524.20230230

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Histological study on the effect of high temperature stress on liver tissue in Larimichthys polyactis

HE Yu¹(), LIU Feng²^,^*(), ZHANG Tianle³, LOU Bao², WEI Fuliang², YE Ting²

1. College of Life Sciences, Huzhou Normal University, Huzhou 313002, Zhejiang, China
2. State Key Laboratory for Managing Biotic and Chemical Threats to the Quality and Safety of Agro-Products, Institute of Hydrobiology, Zhejiang Academy of Agricultural Sciences, Hangzhou 310021, China
3. College of Life Sciences, China Jiliang University, Hangzhou 310018, China

Received:2023-02-25 Online:2024-01-25 Published:2024-02-18

Abstract

Abstract:

In order to explore the effect of high temperature stress on the liver tissue structure and apoptosis of Larimichthys polyactis, the rearing temperature was raised from 15 ℃ to 31 ℃ by altering the water temperature in the tank at a constant rate of 2 ℃·h^-1, and maintained to 96 h, recorded as the test group. At the same time, a control group rearing under the temperature of 15 ℃ was set. At the 0, 6, 12, 24, 48, 72, 96 h after the rearing temperature of the test group reached 31 ℃, the liver tissue of the test and control group were collected to investigate the histological changes and apoptosis of the liver tissues through the methods of tissue sections and terminal-deoxynucleotidyl transferase mediated dUTP nick end labeling (TUNEL), respectively. The results showed that the liver morphology of microstructure and ultrastructure were similar to those of the control group after 0 h treatment at 31 ℃, and there was no significant change. However, with the extension of high temperature treatment time, liver tissue damage gradually worsened. After 96 h of treatment, the liver showed serious damage, including edema, disappearance or even dissolution of nuclei, local necrosis of liver tissue, and destruction of the original structure of liver cells. In addition, with the continuation of high temperature stress, the phenomenon of liver cell apoptosis gradually aggravated. The results showed that high temperature stress caused a certain degree of damage to the liver tissue of L. polyactis and induced a large number of liver cell apoptosis. The results of the present study clarified the effect of high temperature stress on the liver tissue of L. polyactis, which is of great significance for the rearing management of L. polyactis to avoid the occurrence of liver disease.

Key words: Larimichthys polyactis, high-temperature stress, liver, organization structure, apoptosis

CLC Number:

S965.323

HE Yu, LIU Feng, ZHANG Tianle, LOU Bao, WEI Fuliang, YE Ting. Histological study on the effect of high temperature stress on liver tissue in Larimichthys polyactis[J]. Acta Agriculturae Zhejiangensis, 2024, 36(1): 58-66.

Figures/Tables 3

Fig.1 Histology of Larimichthys polyactis liver after high temperature stress (H.E, 400×) A, Control; B, 0 h high temperature stress; C, 6 h high temperature stress; D, 12 h high temperature stress; E, 24 h high temperature stress; F, 48 h high temperature stress; G, 72 h high temperature stress; H, 96 h high temperature stress. CV, Central veins; HC, Hepatocyte; BC, Blood corpuscle; VD, Vacuolation; AB, Gore; N, Nucleus; Oe, Oedema; H, Structural damage.

Fig.2 Ultrastructure of Larimichthys polyactis liver under high temperature stress(4 000×) A, Control; B, 0 h high temperature stress; C, 6 h high temperature stress; D, 12 h high temperature stress; E, 24 h high temperature stress; F, 48 h high temperature stress; G, 72 h high temperature stress; H, 96 h high temperature stress. RER, Rough endoplasmic reticulum; M, Mitochondrion; F, Lipid droplet; SER, Smooth endoplasmic reticulum; L,Lipofuscin; PL,Primary lysosome; SL, Secondary lysosome.

Fig.3 Observation of apoptosis of Larimichthys polyactis liver cells under high temperature stress (400×) A, Control; B, 0 h high temperature stress; C, 6 h high temperature stress; D, 12 h high temperature stress; E, 24 h high temperature stress; F, 48 h high temperature stress; G, 72 h high temperature stress; H, 96 h high temperature stress. Arrows in figure represent apoptotic cells (Brown).

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Histological study on the effect of high temperature stress on liver tissue in Larimichthys polyactis

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