Major gene plus polygene inheritance analysis of curd sitting height in cauliflower

doi:10.3969/j.issn.1004-1524.20230492

Acta Agriculturae Zhejiangensis ›› 2024, Vol. 36 ›› Issue (3): 527-533.DOI: 10.3969/j.issn.1004-1524.20230492

• Horticultural Science • Previous Articles Next Articles

Major gene plus polygene inheritance analysis of curd sitting height in cauliflower

CAI Shiyi¹(), YU Huifang², WANG Jiansheng², ZHU Biao¹, SHEN Yusen², GU Honghui², SHENG Xiaoguang²^,^*()

1. College of Horticulture Science, Zhejiang A&F University, Hangzhou 311300, China
2. Institute of Vegetable, Zhejiang Academy of Agricultural Sciences, Hangzhou 310021, China

Received:2023-04-17 Online:2024-03-25 Published:2024-04-09

Abstract

Abstract:

The “curd sitting height” is one of the important agronomic traits for evaluating the suitability of cauliflower varieties for mechanized harvesting. In order to analyze the genetic pattern of the “curd sitting height” trait in cauliflower, the F₇ inbred line of cauliflower (ZAASC4101, early-maturing and compact type) and the F₆ inbred line of Chinese kale (ZAASJ1401) were used as parents to construct six combined populations including P₁, P₂, F₁, F₂, B₁ and B₂. The “curd sitting height” trait was anchored using two indicators: main stem height and leaf scar spacing. The results of this study showed that there was a significant correlation between the main stem height and the leaf scar spacing in the F₂ population (the correlation coefficient was 0.652). And the two indicators were both continuous and approximately normal distribution, which was consistent with the characteristics of quantitative inheritance. The genetic analysis of the six-generation populations for the main stem height and the F₂ population for the leaf scar spacing both showed that the optimal genetic model of “curd sitting height” trait in cauliflower was two pairs of additive-dominance-epistatic major genes + additive-dominance-epistatic polygenes genetic model, indicating that this trait was mainly controlled by two pairs of major genes + multiple minor genes, and the heritability reached 97.84%. Therefore, it is possible to use the linkage molecular markers to assist selection and genetic improvement of the “curd sitting height ” trait in cauliflower in early generations. In summary, the results of this study laid a preliminary research foundation for further locating and exploring the key genes controlling the “curd sitting height” of cauliflower, and ultimately using biotechnology to cultivate new varieties of cauliflower suitable for mechanized harvesting.

Key words: cauliflower, curd sitting height, six-generation populations, main gene plus polygene genetic analysis, quantitative trait

CLC Number:

S635.3

CAI Shiyi, YU Huifang, WANG Jiansheng, ZHU Biao, SHEN Yusen, GU Honghui, SHENG Xiaoguang. Major gene plus polygene inheritance analysis of curd sitting height in cauliflower[J]. Acta Agriculturae Zhejiangensis, 2024, 36(3): 527-533.

Figures/Tables 6

References 21

[1]	丁海凤, 范建光, 贾长才, 等. 我国蔬菜种业发展现状与趋势[J]. 中国蔬菜, 2020(9): 1-8.
	DING H F, FAN J G, JIA C C, et al. Present situation and trend of vegetable seed industry development in China[J]. China Vegetables, 2020(9): 1-8. (in Chinese)
[2]	李素文, 赵前程, 孙德岭, 等. 国内外花椰菜种植面积及产量变化趋势[J]. 中国蔬菜, 2005(3): 36-37, 62.
	LI S W, ZHAO Q C, SUN D L, et al. Change trend of cauliflower planting area and yield at home and abroad[J]. China Vegetables, 2005(3): 36-37, 62. (in Chinese)
[3]	张倩男, 王克雄, 吴立晓, 等. 松花菜机械化移栽配套提质增效栽培技术[J]. 北方园艺, 2023(1): 151-154.
	ZHANG Q N, WANG K X, WU L X, et al. Cultivation techniques for improving quality and efficiency of mechanized transplanting of Chinese cabbage[J]. Northern Horticulture, 2023(1): 151-154. (in Chinese)
[4]	吕广德, 靳雪梅, 郭营, 等. 小麦株高分子遗传学研究进展[J]. 植物遗传资源学报, 2021, 22(3): 571-582.
	LYU G D, JIN X M, GUO Y, et al. Advances in molecular genetics of wheat plant height[J]. Journal of Plant Genetic Resources, 2021, 22(3): 571-582. (in Chinese with English abstract)
[5]	裘霖琳, 刘窍, 付亚萍, 等. 水稻矮化小穗基因DSP2的鉴定与克隆[J]. 中国水稻科学, 2022, 36(2): 150-158.
	QIU L L, LIU Q, FU Y P, et al. Identification and gene cloning of DSP2 in rice(Oryza sativa L.)[J]. Chinese Journal of Rice Science, 2022, 36(2): 150-158. (in Chinese with English abstract)
[6]	高歌, 杨媛, 郑军, 等. 玉米株高QTL的定位及主效QTL的确认[J]. 核农学报, 2022, 36(8): 1530-1536.
	GAO G, YANG Y, ZHENG J, et al. Mapping of plant height QTL and confirmation of a major QTL in maize[J]. Journal of Nuclear Agricultural Sciences, 2022, 36(8): 1530-1536. (in Chinese with English abstract)
[7]	YAMAGUCHI M, FUJIMOTO H, HIRANO K, et al. Sorghum Dw1, an agronomically important gene for lodging resistance, encodes a novel protein involved in cell proliferation[J]. Scientific Reports, 2016, 6: 28366.
[8]	张晓科, 王辉. 小麦矮源的研究和利用现状[J]. 麦类作物学报, 1996, 16(4): 10-12.
	ZHANG X K, WANG H. Research and utilization status of wheat dwarf sources[J]. Tritical Crops, 1996, 16(4): 10-12. (in Chinese)
[9]	刘超. 甘蓝型油菜矮秆基因Bnrga-ds的克隆和功能分析[D]. 武汉: 华中农业大学, 2010.
	LIU C. Cloning and functional analysis of dwarf gene bnrga-ds in rapeseed (Brassica napus L.)[D]. Wuhan: Huazhong Agricultural University, 2010. (in Chinese with English abstract)
[10]	赵波. 甘蓝型油菜矮秆基因定位、克隆及功能分析[D]. 武汉: 华中农业大学, 2017.
	ZHAO B. Gentic mapping, cloning and functional analysis of dwarf genes in Brassica napus L.[D]. Wuhan: Huazhong Agricultural University, 2017. (in Chinese with English abstract)
[11]	宋晓玉, 甘德芳, 杨琳, 等. 分子标记技术在花椰菜品种鉴定上的应用研究进展[J]. 中国蔬菜, 2022(12): 30-37.
	SONG X Y, GAN D F, YANG L, et al. Research progress in applying molecular marker technology to identify cauliflower varieties[J]. China Vegetables, 2022(12): 30-37. (in Chinese with English abstract)
[12]	SUN D L, WANG C G, ZHANG X L, et al. Draft genome sequence of cauliflower (Brassica oleracea L. var. botrytis) provides new insights into the C genome in Brassica species[J]. Horticulture Research, 2019, 6: 82.
[13]	王五宏, 汪精磊, 李必元, 等. 结球甘蓝抽薹性遗传规律和QTL定位分析[J]. 园艺学报, 2020, 47(5): 974-982.
	WANG W H, WANG J L, LI B Y, et al. Genetic and QTL mapping analysis of bolting time in cabbage(Brassica oleracea)[J]. Acta Horticulturae Sinica, 2020, 47(5): 974-982. (in Chinese with English abstract)
[14]	王靖天, 张亚雯, 杜应雯, 等. 数量性状主基因+多基因混合遗传分析R软件包SEA v2.0[J]. 作物学报, 2022, 48(6): 1416-1424.
	WANG J T, ZHANG Y W, DU Y W, et al. SEA v2.0: an R software package for mixed major genes plus polygenes inheritance analysis of quantitative traits[J]. Acta Agronomica Sinica, 2022, 48(6): 1416-1424. (in Chinese with English abstract)
[15]	盖钧镒. 植物数量性状遗传体系的分离分析方法研究[J]. 遗传, 2005, 27(1): 130-136.
	GAI J Y. Segregation analysis of genetic system of QuantitativeTraits in plants[J]. Hereditas (Beijing), 2005, 27(1): 130-136. (in Chinese with English abstract)
[16]	李光庆, 姚雪琴, 刘春晴, 等. 花椰菜内叶盖球性状的主基因+多基因遗传分析[J]. 上海农业学报, 2017, 33(5): 1-7.
	LI G Q, YAO X Q, LIU C Q, et al. Analysis on cauliflower’s trait for inner leaves to cover curd by means of mixed major gene plus polygene genetic model[J]. Acta Agriculturae Shanghai, 2017, 33(5): 1-7. (in Chinese with English abstract)
[17]	谭华强. 花椰菜绿色花球性状的QTL定位及候选基因分析[D]. 雅安: 四川农业大学, 2020.
	TAN H Q. QTL mapping of green curd trait in cauliflower and analysis of candidate genes[D]. Yaan: Sichuan Agricultural University, 2020. (in Chinese with English abstract)
[18]	何文昭, 王红武, 胡小娇, 等. 玉米株高和穗位高在不同环境下的数量遗传分析[J]. 作物杂志, 2017(3): 13-18.
	HE W Z, WANG H W, HU X J, et al. Quantitative genetic research of plant height and ear height in maize under different environments[J]. Crops, 2017(3): 13-18. (in Chinese with English abstract)
[19]	解松峰, 吉万全, 张耀元, 等. 小麦重要产量性状的主基因+多基因混合遗传分析[J]. 作物学报, 2020, 46(3): 365-384.
	XIE S F, JI W Q, ZHANG Y Y, et al. Genetic effects of important yield traits analysed by mixture model of major gene plus polygene in wheat[J]. Acta Agronomica Sinica, 2020, 46(3): 365-384. (in Chinese with English abstract)
[20]	李英双, 胡丹, 聂蛟, 等. 甜荞株高和茎粗的遗传分析[J]. 作物学报, 2018, 44(8): 1185-1195.
	LI Y S, HU D, NIE J, et al. Genetic analysis of plant height and stem diameter in common buckwheat[J]. Acta Agronomica Sinica, 2018, 44(8): 1185-1195. (in Chinese with English abstract)
[21]	李军庆, 崔翠, 陈雪峰, 等. 油菜半矮秆新种质10D130株高主基因+多基因遗传模型分析[J]. 植物遗传资源学报, 2013, 14(4): 641-646.
	LI J Q, CUI C, CHEN X F, et al. Genetic analysis of rapeseed plant height of new semi-dwaf germplasm 10D130 using major gene plus polygene mixed genetic model[J]. Journal of Plant Genetic Resources, 2013, 14(4): 641-646. (in Chinese with English abstract)

性状	世代	平均值±标准差	株数	变异系数
Traits	Generation	Mean±SD/cm	No. of plants	CV/%
主茎高度	P₁	28.68±0.84	50	2.94
Main stem	P₂	8.12±0.824	50	10.15
height	F₁	20.98±0.79	50	3.79
	F₂	22.95±5.48	219	23.88
	B₁	27.89±2.18	70	7.83
	B₂	20.21±3.52	185	17.42
叶痕间距	P₁	5.47±0.49	50	8.96
Leaf scar	P₂	2.56±0.29	50	11.33
spacing	F₂	4.00±0.98	219	24.50

性状	世代	平均值±标准差	株数	变异系数
Traits	Generation	Mean±SD/cm	No. of plants	CV/%
主茎高度	P₁	28.68±0.84	50	2.94
Main stem	P₂	8.12±0.824	50	10.15
height	F₁	20.98±0.79	50	3.79
	F₂	22.95±5.48	219	23.88
	B₁	27.89±2.18	70	7.83
	B₂	20.21±3.52	185	17.42
叶痕间距	P₁	5.47±0.49	50	8.96
Leaf scar	P₂	2.56±0.29	50	11.33
spacing	F₂	4.00±0.98	219	24.50

六世代群体主茎高度 The height of the main stem in the six-generation population			F₂群体叶痕间距 F₂ group leaf scar spacing
模型Model	MLV	AIC	模型Model	MLV	AIC
1MG-NCD	-1 957.999	3 921.997	1MG-NCD	-301.558 7	611.117 5
1MG-EAD	-1 911.200	3 828.401	1MG-EAD	-298.587 7	605.175 4
1MG-AD	-1 726.858	3 461.716	1MG-AD	-285.367 0	578.734
1MG-A	-1 758.092	3 522.183	1MG-A	-293.133 0	592.265 9
2MG-EAD	-1 802.738	3 611.476	2MG-EAD	-306.559 5	619.118 9
2MG-EA	-1 754.709	3 515.419	2MG-EA	331.458 3	-656.916 6
2MG-CD	-1 802.738	3 613.476	2MG-CD	-306.559 5	621.118 9
2MG-ADI	-1 592.234	3 204.468	2MG-ADI	-295.229 6	610.459 2
2MG-AD	-1 717.980	3 447.959	2MG-AD	346.549 7	-681.099 3
2MG-A	-1 780.579	3 569.157	2MG-A	648.082 0	-1 288.164
PG-ADI	-1 515.611	3 051.222	PG-ADI
PG-AD	-1 754.194	3 522.388	PG-AD
MX1-NCD-AD	-1 677.031	3 370.061	MX1-NCD-AD
MX1-EAD-AD	-1 578.622	3 173.244	MX1-EAD-AD
MX1-AD-ADI	-1 510.622	3 045.244	MX1-AD-ADI
MX1-AD-AD	-1 565.957	3 149.914	MX1-AD-AD
MX1-A-AD	-1 648.965	3 313.931	MX1-A-AD
MX2-EAD-AD	-1 614.513	3 245.025	MX2-EAD-AD
MX2-EA-AD	-1 621.050	3 258.100	MX2-EA-AD
MX2-CD-AD	-1 575.601	3 169.202	MX2-CD-AD
MX2-ADI-ADI	-1 506.296	3 048.592	MX2-ADI-ADI
MX2-ADI-AD	-1 521.620	3 073.240	MX2-ADI-AD
MX2-AD-AD	-1 550.306	3 122.612	MX2-AD-AD
MX2-A-AD	-1 528.941	3 075.882	MX2-A-AD
			0MG	-306.558 7	617.117 4

六世代群体主茎高度 The height of the main stem in the six-generation population			F₂群体叶痕间距 F₂ group leaf scar spacing
模型Model	MLV	AIC	模型Model	MLV	AIC
1MG-NCD	-1 957.999	3 921.997	1MG-NCD	-301.558 7	611.117 5
1MG-EAD	-1 911.200	3 828.401	1MG-EAD	-298.587 7	605.175 4
1MG-AD	-1 726.858	3 461.716	1MG-AD	-285.367 0	578.734
1MG-A	-1 758.092	3 522.183	1MG-A	-293.133 0	592.265 9
2MG-EAD	-1 802.738	3 611.476	2MG-EAD	-306.559 5	619.118 9
2MG-EA	-1 754.709	3 515.419	2MG-EA	331.458 3	-656.916 6
2MG-CD	-1 802.738	3 613.476	2MG-CD	-306.559 5	621.118 9
2MG-ADI	-1 592.234	3 204.468	2MG-ADI	-295.229 6	610.459 2
2MG-AD	-1 717.980	3 447.959	2MG-AD	346.549 7	-681.099 3
2MG-A	-1 780.579	3 569.157	2MG-A	648.082 0	-1 288.164
PG-ADI	-1 515.611	3 051.222	PG-ADI
PG-AD	-1 754.194	3 522.388	PG-AD
MX1-NCD-AD	-1 677.031	3 370.061	MX1-NCD-AD
MX1-EAD-AD	-1 578.622	3 173.244	MX1-EAD-AD
MX1-AD-ADI	-1 510.622	3 045.244	MX1-AD-ADI
MX1-AD-AD	-1 565.957	3 149.914	MX1-AD-AD
MX1-A-AD	-1 648.965	3 313.931	MX1-A-AD
MX2-EAD-AD	-1 614.513	3 245.025	MX2-EAD-AD
MX2-EA-AD	-1 621.050	3 258.100	MX2-EA-AD
MX2-CD-AD	-1 575.601	3 169.202	MX2-CD-AD
MX2-ADI-ADI	-1 506.296	3 048.592	MX2-ADI-ADI
MX2-ADI-AD	-1 521.620	3 073.240	MX2-ADI-AD
MX2-AD-AD	-1 550.306	3 122.612	MX2-AD-AD
MX2-A-AD	-1 528.941	3 075.882	MX2-A-AD
			0MG	-306.558 7	617.117 4

一阶参数Univalent parameter		二阶参数Bivalent parameter
参数 Parameter	估计值 Estimated value	参数 Parameter	估计值 Estimated value
m	23.498 4	σ² mg	4.033 6
d_a	1.727 6	σ² pg	25.229 4
h_a	-0.399 7	h² mg/%	13.485 1
h_b	0.394 6	h² pg/%	84.346 7

Major gene plus polygene inheritance analysis of curd sitting height in cauliflower

RichHTML

PDF (PC)

Knowledge

Abstract

Cite this article

share this article

Figures/Tables 6

References 21

Related Articles 15

Recommended Articles

Metrics

Comments

[1]	HOU Dong, LI Yali, YUE Hongzhong, ZHANG Dongqin, YAO Tuo, HUANG Shuchao, YANG Haixing. Effects of microbial fertilizer instead of partial chemical fertilizer on yield, quality and soil microorganisms of cauliflower [J]. Acta Agriculturae Zhejiangensis, 2024, 36(3): 589-599.
[2]	ZHAI Yilan, ZHANG Chulei, CHU Aixiang, GAO Junge, XIA Qingqing, LU Zhichang. Phenotypic diversity in 27 Acer species [J]. Acta Agriculturae Zhejiangensis, 2023, 35(11): 2621-2635.
[3]	SHENG Xiaoguang, SHEN Yusen, YU Huifang, WANG Jiansheng, ZHAO Zhenqing, GU Honghui. Development and application of KASP marker of BoCAL gene related to curd development in cauliflower [J]. Acta Agriculturae Zhejiangensis, 2022, 34(6): 1183-1192.
[4]	WANG Zhangjun, YAO Mingming, YU Huixia, WANG Yanqing, LI Qingfeng, LIU Fenglou, LIU Caixia, ZHANG Shuangxi, ZHANG Xiaogang, LIU Shengxiang. Construction of genetic map and analysis of QTL for grain protein traits using F_2∶5 pedigrees derived from Ningchun No.4×Hedong black wheat [J]. Acta Agriculturae Zhejiangensis, 2021, 33(8): 1367-1378.
[5]	LI Ju, XIE Bojie, WEI Shouhui, ZHANG Guobin, WU Yue, TANG Zhongqi, XIAO Xuemei, YU Jihua. Effects of combined application of organic fertilizer and chemical fertilizer on nutritional quality and volatile compounds of cauliflower [J]. Acta Agriculturae Zhejiangensis, 2021, 33(7): 1199-1211.
[6]	LI Jinwu, YU Jihua, LYU Jian, FENG Zhi, YANG Haixing, CHE Xusheng, QIN Qijie, ZHANG Yang, JIN Ning. Effects of different mulching methods on yield, quality and soil nutrients of open-air loose-curd cauliflower on plateau in summer [J]. , 2020, 32(9): 1626-1633.
[7]	HUANG Lei, LI Guangqing, YAO Xueqin, LIU Chunqing, XIE Zhujie, GENG Chunnü. Effects of genotype and environment on downy mildew of cauliflower in different developmental stages [J]. , 2020, 32(8): 1420-1426.
[8]	NIU Bo, LI Lina, PANG Guangchang, LU Dingqiang. Construction of plant root tip mesenchymal sensors and their effects on urea sensing dynamics [J]. , 2020, 32(8): 1466-1474.
[9]	WANG Zhangjun, LIU Yan, ZHANG Shuangxi, LIU Fenglou, LI Qingfeng, ZHANG Xiaogang, LIU Shengxiang, JIA Biao. Identification on disease resistance and molecular markers of F₂ hybrids from Ningchun No.4 and Hedong black wheat [J]. , 2019, 31(5): 677-687.
[10]	WANG Xiaomin, ZHAO Yufei, YUAN Dongsheng, LIU Peijun, ZHENG Fushun, HU Xinhua, FU Jinjun, GAO Yanming, LI Jianshe. Analysis of combining ability and heredity for quantitative traits of 33 tomato inbred lines [J]. , 2019, 31(12): 2025-2035.
[11]	WU Wenwen;LU Bing;*. Genetic architecture of quantitative traits based on Shanyou63 recombinant inbred lines [J]. , 2014, 26(2): 0-268273.
[12]	JIANG Zong-qing;CAI Zhi-ling;XIE Ji-xian;SHI Jin-min;LI Xiang-qian;SHI Jyu-qin. Effects of potassium application amount on yield and quality of cauliflower [J]. , 2013, 25(2): 0-327.
[13]	YANG Jia-fu;RAO Li-bing;GU Hong-hui. Analysis of heterosis for agronomic traits in cauliflower (Brassica oleracea L. var. botrytis L.) at different environments [J]. , 2012, 24(3): 0-420.
[14]	ZHAO Zhen-qing;YU Hui-fang;ZHANG Xiao-hui;GU Hong-hui. Advances in DNA markers of cauliflower（Brassica oleracea* var. botrytis L.）and broccoli（Brassica oleracea var. italica L.） [J]. , 2010, 22(2): 0-262.
[15]	CHEN Guo-lin;WU Jian-guo;SHI Chun-hai . Analysis of the genetic effects on the trait of seed amino acid content in crops [J]. , 2009, 21(05): 0-528.