Effects of oyster shells on the growth performance of Penaeus vannamei and water quality, and microbial community characteristics on shell surfaces

doi:10.3969/j.issn.1004-1524.20240840

Acta Agriculturae Zhejiangensis ›› 2025, Vol. 37 ›› Issue (7): 1441-1450.DOI: 10.3969/j.issn.1004-1524.20240840

• Animal Science • Previous Articles Next Articles

Effects of oyster shells on the growth performance of Penaeus vannamei and water quality, and microbial community characteristics on shell surfaces

HUANG Xianke¹^,²^,³(), HUANG Xiaolin¹^,²^,³^,^*(), ZHANG Xiang¹^,²^,³, LI Min¹^,²^,³, CAI Yilong¹^,²^,³, CHEN Ran¹^,²^,³

1. Zhejiang Mariculture Research Institute, Wenzhou 325005, Zhejiang, China
2. Zhejiang Key Laboratory of Coastal Biological Germplasm Resources Conservation and Utilization, Wenzhou 325005, Zhejiang, China
3. Wenzhou Key Laboratory of Marine Biological Genetics and Breeding, Wenzhou 325005, Zhejiang, China

Received:2024-10-08 Online:2025-07-25 Published:2025-08-20

Abstract

Abstract:

In the cultivation of Penaeus vannamei, the addition of oyster shells can improve water quality and enhance shrimp growth. However, the effects of in situ water quality treatment by oyster shells on water quality and shrimp growth, as well as the composition and dynamic succession of microbial communities in oyster shell biofilms, remain inadequately studied. To investigate the effects of adding oyster shells on the growth performance of Penaeus vannamei and aquaculture water quality, this study established a control group and three experimental groups with low (volume fraction 1‰), medium (volume fraction 2‰), and high (volume fraction 4‰) oyster shell additions, comparing their impacts on the growth metrics of Penaeus vannamei and analyzing the distribution characteristics of bacterial communities on the surface of the oyster shells. Results showed that the addition of oyster shells significantly increased the shrimp survival rate (F=9.222, P=0.006), body length (F=13.267, P=0.002), and body weight (F=8.102, P=0.008), the survival rate of the low addition group (90.73%) was significantly lower than that of the medium addition group (97.50%) and the high addition group (99.17%). The addition of oyster shells also increased phosphate content (F=52.509, P<0.001), decreased nitrite content (F=7.323, P=0.011), and increased nitrate content (F=23.615, P <0.001). In the biofilm, the relative abundance of Tenacibaculum significantly decreased as the aquaculture process progressed, while the relative abundance of Nitrospira and Ruegeria significantly increased. The increased relative abundance of Nitrospira in the biofilm accelerated the nitrogen cycle from nitrite to nitrate in the aquaculture system, thereby improving water quality and enhancing shrimp survival rate, body length and body weight. This study provided guidance on the addition of oyster shells in shrimp cultivation and offered a theoretical basis for further exploration of the mechanisms by which oyster shell biofilms improve water quality and promote shrimp health.

Key words: oyster shell, biofilm, Penaeus vannamei, nitrogen cycle, water quality improvement

CLC Number:

S968.22

HUANG Xianke, HUANG Xiaolin, ZHANG Xiang, LI Min, CAI Yilong, CHEN Ran. Effects of oyster shells on the growth performance of Penaeus vannamei and water quality, and microbial community characteristics on shell surfaces[J]. Acta Agriculturae Zhejiangensis, 2025, 37(7): 1441-1450.

Figures/Tables 6

Fig.1 Survival rate, body length and body weight of Penaeus vannamei under different treatments C, Control group; L, Low oyster shell addition; M, Medium oyster shell addition; H, High oyster shell addition. *, P <0.05; **, P<0.01; ***, P<0.001. The same as below.

Fig.2 Hydration indicators under different treatments

Fig.3 Correlation between hydration indicators and Penaeus vannamei growth indicators

Fig.4 Relative abundance of genus-level bacteria in the biofilm on the surface of shell surfaces L, Low oyster shell addition; M, Medium oyster shell addition; H, High oyster shell additions.

Fig.5 Bacterial abundance at the genus level on day 56 L, Low oyster shell addition; M, Medium oyster shell addition; H, High oyster shell additions. *, P <0.05; **, P<0.01; ***, P<0.001.

Fig.6 Correlation between bacterial genus-level abundance and hydration indicators, Penaeus vannamei growth indicators

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Effects of oyster shells on the growth performance of Penaeus vannamei and water quality, and microbial community characteristics on shell surfaces

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