Abstract: Hypocrellin has wide application prospects in the fields of medicine, food and chemical industry. The preparation method of economical and efficient is the basis of its industrialization. Single factor test method was employed in the study for extraction of hypocrellin from the solid fermentation substrate by microwave-assisting. The results showed that the absolute ethyl alcohol was the appropriate solvent, and the productivity of hypocrellin was up to 0.1% in the optimized condition of reaction temperature 50℃, extraction time 7 min, microwave power 600 W, ratio between solvent and substrate 1∶75. Microwave was a remarkable and economical mean in the extraction of hypocrellin from solid fermentation substrate．

Key words: hypocrellin, microwave, productivity, Shiraia bambusicola