Establishment and optimization of SRAP-PCR amplification system in Takifugu obscurus

›› 2011, Vol. 23 ›› Issue (6): 0-1089.

• 动物科学 •

Establishment and optimization of SRAP-PCR amplification system in Takifugu obscurus

CHENG Chanhong;ZHANG Minying;LIU Kai;XU Dongpo;DUAN Jinrong;ZHOU Yanfeng;SHI Weigang;*

1Wuxi Fisheries College of Nanjing Agricultural University,Wuxi 214081, China；2Key Field Station of Observation and Research for Fishery Resources and Environment of the Lower Reaches of Yangtze River, Ministry of Agriculture, Key Laboratory of Ecological Environment and Resources of Inland Fisheries, Freshwater Fisheries Research Center, CAFS, Wuxi 214081, China

Received:1900-01-01 Revised:1900-01-01 Online:2011-11-25 Published:2011-11-25

Abstract

Abstract: Using genomic DNA of Takifugu obscurus, SRAP-PCR amplification system of Takifugu obscurus was optimized using orthogonal design based on five influencing factors of the amplification efficiency (Mg2+, Taq DNA polymerase, dNTPs, DNA template, primer) at four levels. The results showed that the optimal 20 μL SRAP-PCR reaction system contained Mg2+ 1.5mmol·L-1, TaqDNA polymerase 0.5 U, dNTPs 0.15 mmol·L-1, template DNA 75 ng and primer 0.2 μmol·L-1. The optimized SRAP\|PCR system was tested for 30 Takifugu obscurus germplasms and proved to be stable and reliable.

Key words: Takifugu obscurus, SRAP, orthogonal design, optimization

CHENG Chanhong;ZHANG Minying;LIU Kai;XU Dongpo;DUAN Jinrong;ZHOU Yanfeng;SHI Weigang;*. Establishment and optimization of SRAP-PCR amplification system in Takifugu obscurus[J]. , 2011, 23(6): 0-1089.

[1]	YUE Denggao, CHENG Liang, GUO Qingyun. Antibacterial activities and optimization of fermentation conditions of lipopeptides produced by Aureobasidium pullulans PA-2 [J]. Acta Agriculturae Zhejiangensis, 2021, 33(3): 479-489.
[2]	HU Jian, HU Fengjun, ZHANG Hong, ZHU Ying. Optimization of wireless sensor network for water quality monitoring based on improved cuckoo search algorithm [J]. , 2020, 32(5): 897-903.
[3]	ZHANG Hongtao, LI Yijia, TAN Lian, XU Shuaitao. Image recognition of millet leaf disease based on CS-SVM [J]. , 2020, 32(2): 274-282.
[4]	WANG Mingzhe, YANG Ying, TANG Weimin, LIU Zhe, SUN Peilong, LU Shengmin. Optimization of fermentation techniques on yield of exopolysaccharide produced by Lactobacillus pentosae YY112 [J]. , 2020, 32(2): 327-336.
[5]	CHEN Yanli, TANG Zhipeng, OU Kewei, LU Yefei, ZHANG Yu. Optimization of CDDP-PCR reaction system in Fortunella japonica [J]. , 2020, 32(1): 65-71.
[6]	WANG Xiaoxuan, MENG Qingyan, ZHANG Haixiang, WEI Xiangqin, YANG Zenan. Inversion of maize and wheat leaf area index based on particle swarm optimization neural network model [J]. , 2019, 31(7): 1170-1176.
[7]	SONG Zhujun, WU Xuemei, LI Guochang, WANG Fang, LIU Hongyun, ZHANG Fugui, ZHANG Peikun. Optimization of ground wheel components of base fertilizer fixed-point deep applicator [J]. , 2019, 31(5): 829-836.
[8]	JIANG Wu, WU Shiwu, WU Zhigang, TAO Zhengming. Genetic diversity analysis of Gardenia jasminoides in Zhejiang based on SRAP molecular marker technique [J]. , 2019, 31(3): 408-413.
[9]	FAN Ming, XIANG Lu, LIU Zhe, LU Shengmin. Optimization of ultrasonic-assisted technology on mulberry residue extracts with in vitro hypoglycemic activity [J]. , 2019, 31(3): 480-486.
[10]	LI Qi, ZHAO Jie, YANG Liu, WANG Jun, GAO Yixing. Cucumber leaf lesion identification based on GA-BP neural network and feature vector optimization combination [J]. , 2019, 31(3): 487-495.
[11]	MA Lu, KONG Weizhou, WANG Liping, ZHANG Xikang, WANG Cong, LI Peipei, LIU Dunhua. Optimization of processing conditions for venison jerky fermented by response surface methodology and its shelf life prediction [J]. , 2019, 31(12): 2109-2119.
[12]	CHEN Zhi, WANG Yiting, LYU Yongping, MU Haojie, CHEN Jianping. Optimization of aseptic seeding system of Dendrobium officinale Kimura et Migo [J]. , 2018, 30(11): 1870-1878.
[13]	CHU Tingting, ZHU Dequan, ZHU Hong, ZHANG Shun, JIANG Rui, ZHANG Jun. Multidisciplinary collaborative optimization and experiment of screw axis type moving-box mechanism [J]. , 2018, 30(11): 1946-1957.
[14]	ZHANG Anshi, ZHANG Sumin, FAN Dingchen, LIU Ying. Genetic diversity and fingerprints of Gleditsia sinensis germplasm based on SRAP [J]. , 2017, 29(9): 1524-1530.
[15]	DU Xinkai, REN Juan, HU Jun, WANG Changgao, LIN Jianguo, CAI Jun, DU Xin. Prokaryotic expression and purification of sterol carrier protein-2 from Helicoverpa armigera [J]. , 2017, 29(7): 1166-1171.

Establishment and optimization of SRAP-PCR amplification system in Takifugu obscurus

PDF (PC)

Knowledge

Abstract

Cite this article

share this article

References

Related Articles 15

Recommended Articles

Metrics

Comments