Abstract: The HN gene of goose paramyxovirus (GPMV) E03 strain was amplified by RT-PCR with the primers designed on the basis of HN gene sequence of GPMV ZJI strain,and then was inserted into the prokaryotic vector pET32a for the construction of prokaryotic expression vector, named pET-HN. Then we transformed pET-HN into Escherichia coli BL21,and identified by SDS-PAGE and Western blot. The target gene was amplified by RT-PCR. SDS-PAGE and Western blot showed the target strap of fusion protein. It was apparent that the HN gene was expressed in E.coli at high level; meanwhile, the prokaryotic expression products of this gene would be a good foundation for immunologic identification about GPMV．

Key words: Goose paramyxovirus, HN gene, prokaryotic expression