›› 2012, Vol. 24 ›› Issue (4): 0-558.

• 论文 •    

Effects of liposome on immune efficacy of hemorrhage disease of grass carp DNA vaccine

XU Shi-ying;ZOU Yong;WANG Xi-da;LIU Lin;YANG Jia-xin;CHEN Hui;*   

  1. 1 College of Life Sciences, Nanjing Normal University, Nanjing 210046, China; 2 Jiangsu Provincial Key Laboratory for Aquatic Animal Epidemic Prevention and Quarantine, Jiangsu Center for Control and Prevention of Aquatic Animal Infectious Disease, Nanjing 210036, China; 3School of Biology and Basic Medical Sciences, Soochow University, Suzhou 215123, China
  • Received:1900-01-01 Revised:1900-01-01 Online:2012-07-25 Published:2012-07-25

Abstract: Recombinant plasmids pFastBac-β-VP71-VP72, which were used as DNA vaccines based on 30 μg dose, were injected into grass carps by dorsal muscles after being enwrapped by liposome. Transcription situation and antibody level were tested respectively on a regular basis from RTPCR and indirect agglutination reaction for evaluating the role of liposome in comparison with no liposome groups, pFastBacTMDual group and control. The results indicated that transcription of target gene was still in process on the 70th day in liposome group, and antibody level was higher than no liposome groups. Therefore, liposome could promote the immune effect of DNA vaccine and should be further studied and put into practice as a kind of DNA vaccine adjuvant.

Key words: hemorrhage disease of grass carp, DNA vaccine, liposome, immune efficacy