Abstract: In this study， a convenient， sensitive， specific and appreciable LAMP （loop\|mediated isothermal amplification） assay for detection of PCV-2 （porcine circocirus type 2） based on the ORF2 gene was established. This method completed amplification of PCV-2 in the role of the Bst DNA polymerase， by four specific primers designed through six sites of target sequence. After optimization of the reaction mixture and conditions， the assay could successfully detect PCV-2 genomic DNA at 61℃ for 1 h， which was easy to operate， never need PCR instrument and other complex instruments， and the result can be obtained by agarose gel electrophoresis or naked eye. The sensitivity and specificity tests showed that the method was able to detect PCV-2 specifically and its sensitivity could be up to 0.5 pg PCV-2 DNA. The positive rate of LAMP method for PCV-2 was 100% in accordance with international standard of PCR. This study provided an easier， faster way for rapid detection of PCV-2， which met the needs of grass-roots quarantine．

Key words: PCV-2, LAMP, visual observation, quick detection