Rapid detection of citrus bacterial canker by loop-mediated isothermal amplification

doi:10.3969/j.issn.1004-1524.2017.01.16

›› 2017, Vol. 29 ›› Issue (1): 113-118.DOI: 10.3969/j.issn.1004-1524.2017.01.16

• Plant Protection • Previous Articles Next Articles

Rapid detection of citrus bacterial canker by loop-mediated isothermal amplification

GU Yuan¹, CEN Mingsong^{1, 2}, MA Haijie¹, LI Hongye^{1, *}

1. Institute of Biotechnology,College of Agriculture and Biotechnology,Zhejiang University, Hangzhou 310058, China;
2. Hangzhou Plant Protection and Soil-fertilizer Station, Hangzhou 310004, China

Received:2016-06-22 Online:2017-01-15 Published:2017-02-23

Abstract

Abstract: Citrus canker (Xanthomonas citri subsp. citri) is an important quarantine disease. Previous studies showed that loop-mediated isothermal amplification (LAMP) can be used for rapid detection of suspected citrus canker samples. Based on a new conserved genome DNA sequence, we designed LAMP primers and established a LAMP detection method for detection of citrus canker samples in this study. The specificity of established LAMP detection method was consistent with that of regular PCR. However, this LAMP detection method could detect as low as 100 pg DNA of X. citri subsp. citri, and only 4 cells per microliter of X. citri, sensitivity of LAMP was 1 250 times higher than that of the regular PCR. The LAMP assay could distinguish citrus canker from citrus scab, the two diseases easily confused based on symptoms. Therefore, we suggest that LAMP detection method for citrus canker established in this study is simple, rapid, sensitive and specific, it could be used in laboratory with relatively simple conditions.

Key words: citrus canker, Xanthomonas citri subsp. citri, detection, loop-mediated isothermal amplification (LAMP), regular PCR

CLC Number:

S436.661.1

GU Yuan, CEN Mingsong, MA Haijie, LI Hongye. Rapid detection of citrus bacterial canker by loop-mediated isothermal amplification[J]. , 2017, 29(1): 113-118.

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Rapid detection of citrus bacterial canker by loop-mediated isothermal amplification

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