Prokaryotic expression of the recombinant σC protein of new Muscovy duck reovirus and preliminary study of the antigen characteristic

Prokaryotic expression of the recombinant σC protein of new Muscovy duck reovirus and preliminary study of the antigen characteristic

（1 College of Animal Science and Veterinary Medicine，Shandong Agricultural University，Taian 271018，China;2 Institute of Animal Husbandry and Veterinary Sciences， Zhejiang Academy of Agricultural Sciences，Hangzhou 310021，China）

Online:2014-11-25 Published:2014-12-02

Abstract

Abstract: The new Muscovy duck reovirus （N\|DRV） was detected in recently years which could result in serious disease of Muscovy duck. It was different from classical MDRV in host range， characteristics in vitro， clinical pathogenicity and characterization of autoimmunity etc. The σC gene of N\|DRV was amplified by RT\|PCR， digested by NcoⅠand XhoⅠ， and inserted into prokaryotic expression vector pET\|28a（+）. The expression plasmid pET\|28a\|σC was transformed into the competent BL21（DE3）. The fusion protein was expressed after induction and presented mainly in inclusion body. SDS\|PAGE analysis showed that the fusion protein （36 kDa） was expressed correctly. The specific antiserum against σC was produced in rabbit immunized three times by the purified fusion protein σC. The protein σC had high reaction activity through Western Blot and IFA assay.

Key words: new Muscovy duck reovirus, σC gene, prokaryotic expression, rabbit anti serum

CHEN Hai\|peng1，2， YUN Tao2， ZHANG Cun2，*， YU Bin2， NI Zheng2， Hua Jiong\|gang2， CUI Yan\|shun1，*. Prokaryotic expression of the recombinant σC protein of new Muscovy duck reovirus and preliminary study of the antigen characteristic [J]. .

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Prokaryotic expression of the recombinant σC protein of new Muscovy duck reovirus and preliminary study of the antigen characteristic

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