Abstract: An efficient and easy\|to\|use plant expression vector plays an important role in the research of gene functions. In this study， the glyphosate resistant gene CP4\|EPSPS was cloned to replace the original selective marker Bar gene of plant expression vector pBA002. The new plant expression vector was named as pES002. A Trihelix transcription factor GmGT\|2A was subsequently ligated into pES002 to form a new plant expression vector, namely pES002\|GmGT\|2A. This vector was transformed into Arabidopsis by floral dipping method to verify the functions of both CP4\|EPSPS and GmGT\|2A genes. Results showed that the transgenic lines greatly improved the tolerance to both glyphosate and high salinity stress， indicating that both the new selective marker gene and the target gene worked well. Thus， this vector was successfully constructed and could be further used in transgenic related research.

Key words: CP4\, EPSPS, GmGT\, 2A, glyphosate, transcription factor, expression vector