Abstract: In order to optimize the micro\|propagation system of Dendrobium officinale， the aseptic seedlings were used to study the effects of minimal medium， banana amount， activated carbon concentration， NAA concentration， light source， illumination time and temperature on the growth of seedlings through single\|factor experiments. The results showed that the best strengthening medium for seedling growth of Dendrobium officinale was the B5（or improved MS2） with 70 g·L-1 banana， 03 g·L-1 activated carbon， 15 mg·L-1 NAA and 30 g·L-1 sucrose， and the optimum culture condition was at red LED light source， illumination time of 16 h·d-1， and the temperature of （25±1）℃.

Key words: Dendrobium officinale Kimura et Migo, rapid propagation, strong seedlings cultivation, banana, activated carbon, NAA