Arabidopsis N-terminal acetyltransferase Naa50 is involved in regulation of root cell mitosis

doi:10.3969/j.issn.1004-1524.2022.12.03

Acta Agriculturae Zhejiangensis ›› 2022, Vol. 34 ›› Issue (12): 2603-2609.DOI: 10.3969/j.issn.1004-1524.2022.12.03

• Crop Science • Previous Articles Next Articles

Arabidopsis N-terminal acetyltransferase Naa50 is involved in regulation of root cell mitosis

FENG Jinlin(), XI Xiaoyu, ZHAO Shifeng

College of Life Science, Shanxi Normal University, Taiyuan 030031, China

Received:2022-03-22 Online:2022-12-25 Published:2022-12-26

Abstract

Abstract:

N-terminal acetyltransferase Naa50 plays an important role in regulating growth and development in Arabidopsis. In order to explore whether Naa50 affects plant growth and development by regulating cell mitosis, the naa50-1 mutant was crossed with H2B-YFP line, and the segregation behavior of chromosomes during cell division was detected by H2B-YFP. The results showed that compared with the wild-type, the cell mitotic index in root meristem region of naa50-1 mutant decreased, chromosome aberration rate and micronucleus rate increased, and so as the proportion of cells in metaphase increased. The expression of CYCB1 protein in root cells was detected by crossing of naa50-1 mutant with CYCB1-CUS, and it was found that the expression of CYCB1 protein in naa50-1 mutant was increased. PI staining analysis showed that there were several dead cells in the root of naa50-1 mutant. These results indicated that Naa50 promotes the equal distribution of genetic material between two daughter cells during mitosis and the transition from metaphase to anaphase. Overall, Naa50 is involved in the regulation of plant growth and development by regulating cell division.

Key words: Arabidopsis, N-terminal acetyltransferase Naa50, mitotic index, cell mitosis

CLC Number:

Q945.3

FENG Jinlin, XI Xiaoyu, ZHAO Shifeng. Arabidopsis N-terminal acetyltransferase Naa50 is involved in regulation of root cell mitosis[J]. Acta Agriculturae Zhejiangensis, 2022, 34(12): 2603-2609.

Figures/Tables 5

Fig.1 Observation of nuclear phenotype in root tip cells of wild-type and naa50-1 mutant Observation of nuclear morphology of root tip cells in 5-day-old Arabidopsis by using H2B-YFP. Bar=20 μm.

Fig.2 Mitosis and chromosomal aberration of Arabidopsis root apical meristem cells at different stages A, B, C and D are showing the wild-type cells at prophase, metaphase, anaphase and telophase respectively; E, F, G and H are showing the naa50-1 mutant cells at prophase, metaphase, anaphase and telophase respectively; I, Cell with micronucleus; J, Cell with chromosome bridge; K, Cell with multiple bundles of chromosome; L, Asymmetric division cell; M, Cell with plate migration; N, Cell with two nuclei; Bar=5 μm.

Table 1 Effects of Naa50 on chromosome aberration rate and of Arabidopsis root apical meristem cells

基因型 Genotype	TNC	NAC	NCM	NCB	NCC	NAD	CPM	CTN	CFC	PCA/%	PCM/%
野生型Wild-type	3 254	66	3	12	1	10	18	1	20	2.0	0.09
naa50-1	2 265	253	14	17	14	35	130	2	41	11.2	0.62

Table 2 Effects of Naa50 on mitotic rate of Arabidopsis root apical meristem cells

基因型Genotype	TNC	NMS	NCP	NCM	NCA	NCT	MI/%
野生型Wild-type	3254	426	47	20(0.6%)	164	195	13.0
naa50-1	2265	241	38	34(1.5%)	114	55	10.6

Fig.3 Expression of CYCB1 and PI staining in the root cells of Arabidopsis A, Detection of the expression of CYCB1 in the roots of 5-day-old Arabidopsis by using CYCB1-GUS; B, PI staining in the root tips of 5-day-old Arabidopsis; Bar=50 μm.

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Arabidopsis N-terminal acetyltransferase Naa50 is involved in regulation of root cell mitosis

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