Abstract:  MicroRNAs （miRNAs） play a very important role in regulating the stress resistance and growth development. MiR164 is specific in plants， whose target gene is mainly NAC transcription factor family. By utilizing the bioinformatic methods， we analyzed the phylogenetic of miR164 in Vitis vinifera and some other plants， predicted and analyzed the secondary structure of miR164 family members， the base conservation of the mature sequence， as well as the target gene in NAC （71 members） family of Vitis vinifera. The results showed that four members of miR164 in Vitis vinifera were clustered with the dicotyledon plants， which was accorded with evolution law. The precursors of four members could form stable secondary stemloop structure. Otherwise， the base of mature sequence of four members were highly conserved. According to predicting and analyzing of the target gene， we found two NAC members （GSVIVT01007982001 and GSVIVT01020478001）. By BLAST in NCBI， it showed that they were related with plant root development， which indicated that miR164 family and their target gene in Vitis vinifera play important regulatory role in root development and stress resistance.

Key words: Vitis vinifera, miR164, target gene, stress resistance, bioinformatics