Cloning of ZmREM and its expression analysis in maize under stress

doi:10.3969/j.issn.1004-1524.2016.11.03

›› 2016, Vol. 28 ›› Issue (11): 1822-1827.DOI: 10.3969/j.issn.1004-1524.2016.11.03

• Crop Science • Previous Articles Next Articles

Cloning of ZmREM and its expression analysis in maize under stress

WENG Qiao-yun¹, ZHAO Yan-min², ZHANG He¹, SONG Jin-hui¹, MA Hai-lian¹, YUAN Jin-cheng¹, LIU Ying-hui^{1, *}

1. College of Agriculture and Forestry, Hebei North University, Zhangjiakou 075000, China;
2. Zhangjiakou Radio & TV University, Zhangjiakou 075000, China

Received:2016-03-31 Online:2016-11-15 Published:2016-11-16

Abstract

Abstract: The plant-specific B3 superfamily constituted a large transcription factor superfamily. They play central roles in plant development, morphogenesis and stress-resistance. Taking total RNA of maize as template, REM gene was cloned by use the method of reverse transcription polymerase chain reaction (RT-PCR). Length of 1 047 bp cDNA sequence that encoding 349 amino acids was obtained. CD search result showed that it contained two B3-DNA domains and was named as ZmREM. Blast result indicated that ZmREM had 84% and 81% homology with Sorghum bicolor hypothetical protein and Setaria italica B3 domain-containing protein. In various tissues, gene expression in root was the highest. The expression of ZmREM in maize was induced by heat, high-salt, cold and PEG treatment. These results suggested that ZmREM might be a stress related gene of maize.

Key words: maize (Zea mays L.), REM transcription factor, gene cloning, abiotic stress

CLC Number:

S513

WENG Qiao-yun, ZHAO Yan-min, ZHANG He, SONG Jin-hui, MA Hai-lian, YUAN Jin-cheng, LIU Ying-hui. Cloning of ZmREM and its expression analysis in maize under stress[J]. , 2016, 28(11): 1822-1827.

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Cloning of ZmREM and its expression analysis in maize under stress

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