Abstract: To evaluate the effect of immunopotentiator VA5 on immunity potency of inactive vaccine of pseudorabies virus (PRV), inactive vaccine was mixed with VA5. The comparison test of the immunity potency was conducted by using conventional inactive vaccine of Bartha-K61 strain of PRV, inactive vaccine mixed with VA5 and attenuated vaccine of Bartha-K61 strain. Blood samples were collected at 14 and 35 days after the first immunization, respectively. Neutralization experiments and cytokine detection were performed. The results showed that, compared with live and inactive vaccine of Bartha-K61 strain of PRV, the inactive vaccine with VA5 could induce higher neutralizing antibody against the heterologous strain of PRV and increase the expression levels of IL-2 and IFN-γ mRNA in porcine peripheral blood lymphocytes (PBMC). Neutralization test showed that VA5 could significantly increase the level of neutralizing antibody against the epidemic variation strain of PRV. This experiment showed that VA5 could improve the humoral and cellular immunity of inactive PRV vaccine, and provide a basis for paving the better immune effect of inactivated PRV vaccine.

Key words: immunopotentiator, inactive PRV vaccine, Bartha-K61 strain, immunity potency