Bioinformatics and expression analysis of 5 newfound ERF genes in soybean

doi:10.3969/j.issn.1004-1524.2016.10.03

›› 2016, Vol. 28 ›› Issue (10): 1644-1649.DOI: 10.3969/j.issn.1004-1524.2016.10.03

• Crop Science • Previous Articles Next Articles

Bioinformatics and expression analysis of 5 newfound ERF genes in soybean

ZHAI Ying¹, ZHANG Jun², ZHAO Yan¹, GAO Shi-tong¹, SUN Wan-shu¹, ZHANG Chuang¹, REN Wei-wei¹, WANG Xiu-wen¹, WANG Yu-shu¹

1. College of Life Science and Agro-forestry, Qiqihar University, Qiqihar 161006, China;
2. Heilongjiang Institute of Veterinary Science, Qiqihar 161005, China

Received:2016-02-16 Online:2016-10-15 Published:2016-11-20

Abstract

Abstract: ERF transcription factors are widespread in plants, which are widely involved in plant response to biotic and abiotic stress. In this study, five novel ERF gene sequences, ERFa/b/c/d/e, were obtained by blast from NCBI data base. Protein sequences analysis showed that the five ERF proteins all contained a typical AP2/ERF binding domain. Phylogenetic analysis indicated that GmERFe was highly homologous to GmERF3 and GmERF7; GmERFb and GmERFc were highly homologous to GmERF5; GmERFd was homologous to GmEREBP1; while GmERFa had lower homology with other ERF proteins. The expression patterns of five ERF genes in soybean tissues and under stress treatments were analyzed by real-time quantitative PCR. The results showed that the five ERF genes expressed highly in root and leaf. GmERFd/e responded mainly to ethylene signal and low temperature, GmERFb responded mainly to drought, and GmERFa responded mainly to low temperature.

Key words: soybean, ERF transcription factor, bioinformatics analysis, expression analysis

CLC Number:

S565.1

ZHAI Ying, ZHANG Jun, ZHAO Yan, GAO Shi-tong, SUN Wan-shu, ZHANG Chuang, REN Wei-wei, WANG Xiu-wen, WANG Yu-shu. Bioinformatics and expression analysis of 5 newfound ERF genes in soybean[J]. , 2016, 28(10): 1644-1649.

References

[1] 崔喜艳, 陈众峰, 陈展宇. AP2/ERF转录因子对植物非生物胁迫应答的研究进展[J]. 吉林农业大学学报, 2015, 37(4): 417-423.
CUI X Y, CHEN Z F, CHEN Z Y. Advances of researches on AP2/ERF transcription factor response to plant abiotic stress[J]. Journal of Jilin Agricultural University , 2015, 37(4): 417-423. (in Chinese with English abstract)
[2] UPADHYAY R K, SONI D H, SINGH R, et al. SIERF 36, an EAR-motif-containing ERF gene from tomato, alters stomatal density and modulates photosynthesis and growth[J]. Journal of Experimental Botany , 2013, 64(11): 3237-3247.
[3] 张淑珍, 华彩峰, 董利东, 等. ERF转录因子及在大豆中的研究进展[J]. 大豆科学, 2015, 34(3): 512-517.
ZHANG S Z, HUA C F, DONG L D, et al. ERF transcription factors and their research advancement in soybean[J]. Soybean Science , 2015, 34(3): 512-517. (in Chinese with English abstract)
[4] THIRUGNANASAMBANTHAM K, DURAIRAJ S, SARAVANAN S, et al. Role of ethylene response transcription factor (ERF) and its regulation in response to stress encountered by plants[J]. Plant Molecular Biology Reporter , 2015, 33(3): 347-357.
[5] OHME-TAKAGI M, SHINSHI H. Ethylene-inducible DNA binding proteins that interact with an ethylene-responsive element[J]. Plant Cell , 1995, 7(2): 173-182.
[6] HAO D, OHME-TAKAGI M, SARAI A. Unique mode of GCC box recognition by the DNA-binding domain of ethylene-responsive element binding factor(ERF domain) in plants[J]. Journal of Biological Chemistry , 1998, 273(41): 26857-26861.
[7] PARK J M, PARK C J, LEE S B, et al. Overexpression of the tobacco Tsi1 gene encoding an EREBP /AP2-type transcription factor enhances resistance against pathogen attack and osmotic stressing tobacco[J]. Plant Cell , 2001, 13(5): 1035-1046.
[8] BUTTNER M, SINGH K B. Arabidopsis thaliana ethylene-responsive element binding protein (AtEBP), an ethylene-inducible, GCC box DNA-binding protein interacts with an ocs element binding protein[J]. Proceedings of the National Academy of Sciences of the United States of America , 1997, 94(11): 5961-5966.
[9] XUE G P, LOVERIDGE C W. HvDRF1 is involved in abscisic acid mediated gene regulation in barley and produces two forms of AP2 transcriptional activators, interacting preferably with a CT-rich element[J]. Plant Journal , 2004, 37(3): 326-339.
[10] FUJIMOTO S Y, OHTA M, USUI A, et al. Arabidopsis ethylene-responsive element binding factors act as transcriptional activators or repressors of GCC box-mediated gene expression[J]. Plant Cell , 2000, 12(3): 393-404.
[11] XU Z S, XIA L Q, CHEN M, et al. Isolation and molecular characterization of the Triticum aestivum L. ethylene-responsive factor 1 ( TaERF 1) that increases multiple stress tolerance[J]. Plant Molecular Biology , 2007, 65(6): 719-732.
[12] ZHAI Y, WANG Y, LI Y J, et al. Isolation and molecular characterization of GmERF 7, a soybean ethylene-response factor that increases salt stress tolerance in tobacco[J]. Gene , 2013, 513(1): 174-183.
[13] ZHAI Y, LI J W, LI X W, et al. Isolation and characterization of a novel transcriptional repressor GmERF 6 from soybean[J]. Biologia Plantarum , 2013, 57(1): 26-32.
[14] OHTA M, MATSUI K, HIRATSU K, et al. Repression domains of class II ERF transcriptional repressors share an essential motif for active repression[J]. Plant Cell , 2001, 13(8): 1959-1968.
[15] DONG L D, CHENG Y X, WU J J, et al. Overexpression of GmERF 5, a new member of the soybean EAR motif-containing ERF transcription factor, enhances resistance to Phytophthora sojae in soybean[J]. Journal of Experimental Botany , 2015, 66(9): 2635-2647.
[16] ZHANG G Y, CHEN M, CHEN X P, et al. Phylogeny, gene structures, and expression patterns of the ERF gene family in soybean ( Glycine max L.) [J]. Journal of Experimental Botany , 2008, 59(15): 4095-4107.
[17] ZHANG Y Y, YANG C W, LI Y, et al. SDIR1 is a RING finger E3 ligase that positively regulates stress-responsive abscisic acid signaling in Arabidopsis [J]. Plant Cell , 2007, 19(6): 1912-1929.
[18] TOURNIER B, SANCHEZ-BALLESTA M T, JONES B, et al. New members of the tomato ERF family show specific expression pattern and diverse DNA-binding capacity to the GCC box element[J]. FEBS Letters , 2003, 550(1-3): 149-154.
[19] ZHANG G Y, CHEN M, LI L C et al. Overexpression of the soybean GmERF 3 gene, an AP2/ERF type transcription factor for increased tolerances to salt, drought, and diseases in transgenic tobacco[J]. Journal of Experimental Botany , 2009, 60(13): 3781-3796.
[20] ONATE-SANCHEZ L, SINGH K B. Identification of Arabidopsis ethylene-responsive element binding factors with distinct induction kinetics after pathogen infection[J]. Plant Physiology , 2002, 128(4): 1313-1322.

Bioinformatics and expression analysis of 5 newfound ERF genes in soybean

RichHTML

PDF (PC)

Knowledge

Abstract

Cite this article

share this article

References

Related Articles 15

Recommended Articles

Metrics

Comments

[1]	ZHAO Xiuping, WANG Shuang, YAN Xingyi, DUAN Qiang, ZHANG Shuai, CHEN Yongsheng, LI Guorui. Expression, purification and bioinformatics analysis of Magnaporthe oryzae MGG-01005 [J]. Acta Agriculturae Zhejiangensis, 2021, 33(3): 470-478.
[2]	HE Jiaqi, ZHAI Ying, ZHANG Jun, QIU Shuang, LI Mingyang, ZHAO Yan, ZHANG Meijuan, MA Tianyi. Cloning and expression analysis of GmDof1.5 in soybean under abiotic stress [J]. Acta Agriculturae Zhejiangensis, 2021, 33(1): 1-7.
[3]	YANG Yiwei, XIAO Hua, CHEN Hu, XIAO Niejia, GUO Cheng. Structural characteristics of soil mite communities under different modes of rose-based agroforestry in Karst area [J]. Acta Agriculturae Zhejiangensis, 2021, 33(1): 112-121.
[4]	WANG Weike, LU Na, YAN Jing, SONG Jiling, YUAN Weidong, ZHOU Zufa. Cloning and expression analysis of PpSAMS gene of Pleurotus pulmonarius [J]. Acta Agriculturae Zhejiangensis, 2021, 33(1): 62-68.
[5]	LIANG Liqin, YANG Rui, GAO Gang. Bioinformatics analysis of StUOXs gene family in potato [J]. , 2020, 32(9): 1523-1532.
[6]	WU Jia, CHEN Lang, JIANG Tao, HUANG Guoming, LI Zhuo, LI Yaodong, ZHANG Li, LIU Lixia. Genetic polymorphism screening of CSF3 gene in dairy cow and its bioinformatics analysis [J]. , 2020, 32(6): 986-993.
[7]	ZHANG Guwen, SHEN Li, ZHENG Huazhang, LIU Na, FENG Zhijuan, GONG Yaming. Research advances of zinc finger protein transcription factor Di19 in regulation of soybean responding to drought stress [J]. , 2020, 32(2): 373-382.
[8]	WANG Weike, SONG Jiling, LU Na, YUAN Weidong, YAN Jing, CHEN Guanping. Cloning and expression of the PpFBD1 involved in primordium formation of Pleurotus pulmonarius [J]. , 2020, 32(1): 93-97.
[9]	JIANG Fengqian, LI Yang, YU Dawei, SUN Min, ZHANG Enbao. Soybean disease detection system based on convolutional neural network under Caffe framework [J]. , 2019, 31(7): 1177-1183.
[10]	LIU Zhengkui, WU Yuan, CHEN Lin, WANG Lei, MU Hongye, ZHU Xuhang, WANG Xiaodu. Prokaryotic expression and bioinformatics analysis of Nsp5 gene of porcine epidemic diarrhea virus [J]. , 2019, 31(4): 532-538.
[11]	DONG Xinxing, LI Mingli, CUI Yijia, LAN Guoxiang, WANG Xiaoyi, YAN Dawei. Cloning, bioinformatics analysis and tissue expression detection of MCUR1 gene in Saba pig [J]. , 2019, 31(11): 1825-1833.
[12]	LIU Peng, JIN Chengqian, YIN Xiang, NING Xinjie, LI Qinglun. Research progress of soybean combine harvester cleaning equipment and key technologies [J]. , 2019, 31(10): 1758-1766.
[13]	WANG Yushu, WANG Huan, GUO Yu, ZHOU Minghui, CHEN Lu, CHEN Yang. Cloning and expression analysis of β-carotene hydroxylase gene from kale (Brassica oleracea L. var. acephala) [J]. , 2019, 31(1): 80-85.
[14]	HU Zhihui, WANG Yanjie, ZHANG Liqin. Prediction of vegetable soybean yield with fluorescence, spectra, photosynthetic parameters after fertilization [J]. , 2018, 30(8): 1355-1362.
[15]	WU Wei, FENG Zhijuan, XU Shengchun, LIU Na, ZHANG Guwen, HU Qizan, GONG Yaming. Genome-wide identification and expression analysis of soybean NIPs [J]. , 2018, 30(7): 1101-1109.