Identification and characterization of new tea line Lizaoxiang based on chemical fingerprint

doi:10.3969/j.issn.1004-1524.2016.10.14

›› 2016, Vol. 28 ›› Issue (10): 1724-1729.DOI: 10.3969/j.issn.1004-1524.2016.10.14

• Horticultural Science • Previous Articles Next Articles

Identification and characterization of new tea line Lizaoxiang based on chemical fingerprint

PAN Jian-yi¹, CHENG Hao^{2, *}, WANG Li-yuan², MA Jun-hui¹, CHEN Xiang-yun³

1. Crop Workstation of Agriculture Bureau of Lishui, Lishui 323000, China;
2. Tea Research Institute of Chinese Academy of Agricultural Sciences, National Center for Tea Improvement, Hangzhou 310008, China;
3.Forest Workstation of Gaochun District of Nanjing, Jiangsu, Nanjing 211300, China

Received:2016-06-16 Online:2016-10-15 Published:2016-11-20

Abstract

Abstract: Chemical fingerprint technology has widely been implicated for discriminating the identity and origin of tea plant. Difference identification between tea plant cultivars is important for applying for new variety certification. In the current study, we analyzed the chemical composition and proportion of bioactive compounds using HPLC and built a chemical fingerprint to distinguish Lizaoxiang from Longjing 43, Yingshuang and Jiukeng. On the basis of the obtained HPLC chromatographic data, we selected common peak data of more than 90% samples to build chemical fingerprint chromatograph for further analysis. Chromatography revealed that Lizaoxiang was closest to Jiukeng;while the proportion of catechins, gallic acid and caffeine was different from that of Longjing 43, Yingshuang and Jiukeng. These observations help to distinguish Lizaoxiang from Longjing 43, Yingshuang and Jiukeng, and thus provid reference to characterize the difference between the varieties of tea plant for new variety protection.

Key words: tea, Lizaoxiang, chemical fingerprint, identification analysis

CLC Number:

S571.1

PAN Jian-yi, CHENG Hao, WANG Li-yuan, MA Jun-hui, CHEN Xiang-yun. Identification and characterization of new tea line Lizaoxiang based on chemical fingerprint[J]. , 2016, 28(10): 1724-1729.

References

[1] 沈伟, 张一文, 李赫宇. 大川芎方 HPLC 指纹图谱方法学研究[J]. 食品研究与开发, 2015, 36(18): 159-161.
SHEN W, ZHANG Y W, LI H Y. The HPLC fingerprint analysis of dachuanxiong formula[J]. Food Research and Development , 2015, 36(18):159-161.(in Chinese with English abstract)
[2] 李静, 乔媛媛, 魏屹,等. 四川地区峨参高效液相指纹图谱研究[J]. 江苏农业科学, 2013, 41(10): 267-269.
LI J, QIAO Y Y, WEI Y, et al. The HPLC fingerprint analysis of wild chervil in Sichuan[J]. Jiangsu Agriculture Sciences , 2013, 41(10): 267-269. (in Chinese with English abstract)
[3] 周建良, 齐炼文, 李萍. 色谱指纹图谱在中药质量控制中的应用[J]. 色谱, 2008, 26(2): 153-159.
ZHOU J L, QI L W, LI P. Quality control of Chinese herbal medicines with chromatographic fingerprints[J]. Chinese Journal of Chromatography , 2008, 26(2): 153-159. (in Chinese with English abstract)
[4] 王丽鸳, 成浩, 周健,等. 基于多元化学指纹图谱的武夷岩茶身份判别研究[J]. 茶叶科学, 2010, 30(2): 83-88.
WANG L Y, CHENG H, ZHOU J, et al. Discriminant classification of Wuyi Yan tea based on multiple chemical fingerprint[J]. Journal of Tea Science , 2010, 30(2):83-88. (in Chinese with English abstract)
[5] 成浩, 王丽鸳, 周健,等. 基于化学指纹图谱的扁形茶产地判别分析研究[J]. 茶叶科学, 2008, 28(2): 83-88.
CHENG H, WANG L Y, ZHOU J, et al. Discriminant classification of production area of flatten-shaped green tea based on multiple chemical fingerprint[J]. Journal of Tea Science , 2008, 28(2):83-88. (in Chinese with English abstract)
[6] 王丽鸳, 成浩, 周健,等. 绿茶数字化多元化学指纹图谱建立初探[J]. 茶叶科学, 2007, 27(4): 335-342.
WANG L Y, CHENG H, ZHOU J, et al. A preliminary attempt to establish the digital multiple chemical fingerprints of green tea[J]. Journal of Tea Science , 2007, 27(4): 335-342. (in Chinese with English abstract)
[7] 程翼宇, 陈闽军, 吴永江. 化学指纹图谱的相似性测度及其评价方法[J]. 化学学报, 2002, 60(11): 2017-2021.
CHENG Y Y, CHEN M J, WU Y J. Measures for determining the similarity of chemical fingerprint and a method of evaluating the measures[J]. Acta Chemical Sinica , 2002, 60(11): 2017-2021. (in Chinese with English abstract)
[8] 黄丹娟, 马建强, 陈亮. 茶树 DNA 分子指纹图谱研究进展[J]. 茶叶科学, 2015, 35(6): 513-519.
HUANG D J, MA J Q, CHEN L. Research progress on DNA molecular fingerprinting of tea plant( Camellia sinensis ) [J]. Journal of Tea Sciences , 2015, 35(6): 513-519. (in Chinese with English abstract)
[9] 刘英, 吴曙光, 尹州,等. 指纹图谱技术在茶叶研究上的应用[J]. 茶叶科学, 2013, 33(1): 13-20.
LIU Y, WU S G, YIN Z, et al. Application of fingerprint technique in tea research[J]. Journal of Tea Sciences , 2013, 33(1): 13-20. (in Chinese with English abstract)
[10] 范杰文, 罗一帆, 凌彩金. 茶叶指纹图谱研究进展[J]. 广东农业科学, 2013, 40(14): 228-231.
FAN J W, LUO Y F, LING C J. Research progress of tea fingerprint[J]. Guangdong Agriculture Sciences , 2013, 40(14): 228-231. (in Chinese with English abstract)
[11] 贺巍. 基于化学指纹图谱的茶叶产地、原料品种判别分析和生化成分预测[D]. 南京:南京农业大学, 2011.
HE W. Discriminant analysis of production regions and tea plant cultivars, and prediction of chemistry components based on chemical fingerprint[D]. Nanjing: Nanjing Agricultural University, 2011. (in Chinese with English abstract)
[12] 成浩, 王丽鸳, 周健,等. 基于化学指纹图谱的绿茶原料品种判别分析[J]. 中国农业科学, 2008, 41(8): 2413-2418.
CHENG H, WANG L Y, ZHOU J, et al. Discriminant classification of green tea’s raw material cultivar based on multiple fingerprint[J]. Scientia Agricultura Sinica , 2008, 41(8):2413-2418. (in Chinese with English abstract)

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Identification and characterization of new tea line Lizaoxiang based on chemical fingerprint

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