Studies on the detached-leaf inoculation method for determination of rice resistance to sheath blight (Rhizoctonia solani)

doi:10.3969/j.issn.1004-1524.2016.10.15

›› 2016, Vol. 28 ›› Issue (10): 1730-1737.DOI: 10.3969/j.issn.1004-1524.2016.10.15

• Plant Protection • Previous Articles Next Articles

Studies on the detached-leaf inoculation method for determination of rice resistance to sheath blight (Rhizoctonia solani)

MA Chen-yan^{1, 2}, YUAN Zheng-jie², YANG Hai-he^{1, 2}, QU Hai-yan^{1, 2}, HE Hai-yan², ZHANG Yu², QU Shao-hong^{2, *}

1. College of Chemistry and Life Sciences, Zhejiang Normal University, Jinhua 321004, China;
2. Institute of Virology and Biotechnology, Zhejiang Academy of Agricultural Sciences, Hangzhou 310021, China

Received:2016-03-15 Online:2016-10-15 Published:2016-11-20

Abstract

Abstract: Rice sheath blight (Rhizoctonia solani) is one of the important diseases causing large loss to rice production. Rice resistance to R. solani belongs to quantitative genetic trait. Rice germplasm with high resistance or immunity to sheath blight has not yet been identified. In order to improve the efficiency of screening and characterizing sheath blight-resistant rice germplasm, we made precise control of the experimental conditions for the detached-leaf inoculation method, and developed a quantitative protocol for the evaluation of disease severity. The detached leaf tissues inoculated with R. solani were subjected to quantitative reverse-transcription qRT-PCR analysis. The induced expression of rice pathogenesis-related genes verified the infection of the pathogen into rice. A sheath blight-resistant rice cultivar and a sensitive cultivar were tested using the detached-leaf method, the field inoculation method and the micro-chamber inoculation method, respectively. The three methods showed similar results, and disease scores of the resistant and sensitive cultivars were significantly different based on statistical tests. Taken together, the improved detached-leaf inoculation method had the advantages of easy operation and good repeatability, and could be used for large-scale screening of sheath blight-resistant rice germplasm.

Key words: rice (Oryza sativa), sheath blight, Rhizoctonia solani, detached-leaf inoculation, pathogenesis-related gene

CLC Number:

S511

MA Chen-yan, YUAN Zheng-jie, YANG Hai-he, QU Hai-yan, HE Hai-yan, ZHANG Yu, QU Shao-hong. Studies on the detached-leaf inoculation method for determination of rice resistance to sheath blight (Rhizoctonia solani)[J]. , 2016, 28(10): 1730-1737.

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Studies on the detached-leaf inoculation method for determination of rice resistance to sheath blight (Rhizoctonia solani)

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