Photosynthetic physiological responses of Dioscorea opposite Thunb. cv. Guangfeng Qianjin acclimated and transplanted plantlets under PEG drought stress

doi:10.3969/j.issn.1004-1524.2016.09.03

›› 2016, Vol. 28 ›› Issue (9): 1462-1475.DOI: 10.3969/j.issn.1004-1524.2016.09.03

• Crop Science • Previous Articles Next Articles

Photosynthetic physiological responses of Dioscorea opposite Thunb. cv. Guangfeng Qianjin acclimated and transplanted plantlets under PEG drought stress

KE Wei-zhong, WANG Li, YANG Xing-peng, XU Yu-qin, WU Dan, JAING Xia, XIA Jin-hua, YIN Ming-hua, HONG Sen-rong^*

College of Life Sciences, Shangrao Normal University, Shangrao 334001, China

Received:2015-12-28 Online:2016-09-15 Published:2016-11-23

Abstract

Abstract: In order to explore the drought-resistant physiological mechanism of Dioscorea opposite Thunb. cv. Guangfeng Qianjin acclimated and transplanted plantlets, its photosynthetic physiological responses under drought stress were tested in this paper. The results showed that 50 and 100 g·L^-1 PEG-6000 could significantly promote the biomass and root-shoot ratio of D. opposite Thunb. cv. Guangfeng Qianjin acclimated and transplanted plantlets, while 150 and 200 g·L^-1 PEG-6000 could significantly reduced the biomass and root-shoot ratio. When PEG-6000 concentration was 50 and 100 g·L^-1, the leaf relative water content, stomatal aperture, Tr, Fo, Fv/Fm, Φ_PSⅡ, NPQ and ZR had no significant changes, the chlorophyll content, stomatal density, Pn, Gs, Ls, WUE, CUE, Fm, Fv/Fo, Fv'/Fm', IAA, GA₃ and ABA increased significantly, Ci decreased significantly. When PEG-6000 concentration reached 150 and 200 g·L^-1, the leaf relative water content, chlorophyll content, stomatal aperture, Pn, Gs, Ci, Tr, WUE, CUE, Fo, Fm, Fv/Fo, Φ_PSⅡ, Fv'/Fm', Fv/Fm, qP, GA₃, ZR and IAA decreased significantly, stomatal density, Ls, NPQ and ABA increased significantly. Under drought stress, ZR/ABA ratio and ZR/IAA ratio of D. opposite Thunb. cv. Guangfeng Qianjin acclimated and transplanted plantlets decreased significantly with the increase of PEG-6000 concentration, while the ABA/ (IAA+GA3+ZR) increased significantly with the increase of PEG-6000 concentration. The FCM test results of DNA content showed that the ploidy of D. opposite Thunb. cv. Guangfeng Qianjin acclimated and transplanted plantlets under PEG drought stress was stable, but when PEG-6000 concentration reached 200 g·L^-1, there were clear DNA ladders in the electrophoretic pattern. The results showed that D. opposite Thunb. cv. Guangfeng Qianjin acclimated and transplanted plantlets could adapt to a certain degree of drought stress, but with the aggravation of drought stress, its growth and development, photosynthesis and endogenous hormone regulation mechanism would be affected, which would lead to programmed cell death.

Key words: Dioscorea opposite Thunb. cv. Guangfeng Qianjin, acclimated and transplanted plantlets, drought stress, photosynthetic physiological responses

CLC Number:

S632.1

KE Wei-zhong, WANG Li, YANG Xing-peng, XU Yu-qin, WU Dan, JAING Xia, XIA Jin-hua, YIN Ming-hua, HONG Sen-rong. Photosynthetic physiological responses of Dioscorea opposite Thunb. cv. Guangfeng Qianjin acclimated and transplanted plantlets under PEG drought stress[J]. , 2016, 28(9): 1462-1475.

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Photosynthetic physiological responses of Dioscorea opposite Thunb. cv. Guangfeng Qianjin acclimated and transplanted plantlets under PEG drought stress

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