Epitope analysis of cow mastitis important vaccine candidate proteins and design of amino acid sequence of triple recombinant epitope vaccine

doi:10.3969/j.issn.1004-1524.2016.09.04

›› 2016, Vol. 28 ›› Issue (9): 1476-1484.DOI: 10.3969/j.issn.1004-1524.2016.09.04

• Animal Science • Previous Articles Next Articles

Epitope analysis of cow mastitis important vaccine candidate proteins and design of amino acid sequence of triple recombinant epitope vaccine

LIU Xiang¹, KONG Zhi-xiang¹, YIN Shu-ping², OU Sha-sha¹, LIU Cheng-yan¹, TONG Han-hu¹, WU San-qiao¹, CHEN Chen¹, CHEN Chun-lin¹

1. Chinese-German Joint Institute for Natural Product Research, Shaanxi University of Technology, Hanzhong 723001, China;
2. Shaanxi Pharmaceutical Holding Group Co., Ltd, Hanzhong 723000, China

Received:2015-12-22 Online:2016-09-15 Published:2016-11-23

Abstract

Abstract: In order to design cow mastitis triple recombinant epitope polypeptide vaccine, six candidate vaccine proteins were selected, which were from three main pathogens of cow mastitis, concluding Ebps and ClfA of Staphylococcus aureus, OmpA and OmpC of Escherichia coli, SIP and PGK of Streptococcus. Using ABCpred and BepiPred prediction program, each protein had 2 B cell epitopes. CTL cell epitopes were obtained by the prediction method of quantitative matrix and artificial neural network, the results showed that the SIP protein had no CTL cell epitope, and there was 1 CTL cell epitope to other proteins. Th cell epitopes were gained using an online server prediction for MHC class Ⅱ peptide binding affinity, the results showed that each protein had 1 Th cell epitope. DNASTAR software was used to analyze the secondary structure for the six proteins, and the results showed that most of the B/T cell epitopes were located on the exposed surface, the random coil and the corner. The B/T cell epitopes were recomposed by DNASTAR Protean software, and triple epitope vaccine amino acid sequence holding better antigen was designed finally. These laid the foundation for the novel triple epitope vaccine of cow mastitis.

Key words: cow mastitis, epitope vaccine, antigenic analysis

CLC Number:

S858.23

LIU Xiang, KONG Zhi-xiang, YIN Shu-ping, OU Sha-sha, LIU Cheng-yan, TONG Han-hu, WU San-qiao, CHEN Chen, CHEN Chun-lin. Epitope analysis of cow mastitis important vaccine candidate proteins and design of amino acid sequence of triple recombinant epitope vaccine[J]. , 2016, 28(9): 1476-1484.

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Epitope analysis of cow mastitis important vaccine candidate proteins and design of amino acid sequence of triple recombinant epitope vaccine

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