›› 2016, Vol. 28 ›› Issue (9): 1485-1492.DOI: 10.3969/j.issn.1004-1524.2016.09.05

• Animal Science • Previous Articles     Next Articles

Effects of obesity on blood physiological parameters, four cytokines and immune organs index in response to nonfatal pneumonia in mice

WAN Tao-mei, YUAN Gui-qiang, WANG Zheng-yi, JIA Yi-ping, REN Yi, ZUO Zhi-cai*   

  1. Key Laboratory of Animal Disease and Human Health of Sichuan Province/ Key Laboratory of Environmental Hazard and Animal Disease of Sichuan Province/ College of Veterinary Medicine,Sichuan Agricultural University,Wenjiang 611130,China
  • Received:2015-12-03 Online:2016-09-15 Published:2016-11-23

Abstract: To investigate the impact of obesity on systemic immune responses of nonfatal pneumonia, high-fat induced obese mice were divided into group Ⅰ, Ⅱ, and the mice fed with standard diet were divided into group Ⅲ, Ⅳ. Group Ⅰ, Ⅲ were infused intranasally by 40 μL bacteria solution containing 4×109 cfu Escherichia coli (E.coli), and group Ⅱ, Ⅳ were given the same dose of saline. The blood physiological parameters, cytokines in serum and indexes of immune organs of all groups were detected at 2, 6, 12, 24, 48, 72 and 96 h post-infection. As a result, fed with high-fat diet for 8 weeks, the mice had greater WBC, GRA, MID counts, higher concentrations of TNF-α, IL-6, IL-10 and resistin, and lower indexes of spleen and thymus. After infection, indexes of spleen in group Ⅰ were higher than those in group Ⅱ during 2 to 72 h, and in group Ⅲ at 2, 6, 48, 72 and 96. Indexes of thymus in groups Ⅰ were lower than those in group Ⅱ at 6, 12 h and in group Ⅲ at 72, 96 h post-infection. During infection, WBC, GRA, MID counts increased firstly, then decreased, finally decreased to the controls in group Ⅰ, Ⅲ, and were both lower than those in control groups at 24 h post-infection. In the experimental period, WBC, GRA, MID counts in group Ⅰ were higher than those in group Ⅲ. The concentrations of TNF-α, IL-6, IL-10 and resistin elevated at 2 h post-infection, and maintained high concentrations to 48 h post-infection except resistin in group Ⅲ. The four cytokines in group Ⅰ were higher than those in group Ⅲ at 2, 6 h, and lower at 96 h post-infection. The results revealed that experimental mice presented systemic inflammatory responses during nonfatal pneumonia, and obesity enhanced the immune responses to inflammation to promote the abreaction of inflammation.

Key words: obesity, nonfatal pneumonia, blood physiological parameters, cytokine, immune organs index

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