Effects of DON and ZEA on IL-2, IL-4, IL-10 and IFN-γ of chicken spleen lymphocytes cultured in vitro

doi:10.3969/j.issn.1004-1524.2018.07.02

›› 2018, Vol. 30 ›› Issue (7): 1110-1115.DOI: 10.3969/j.issn.1004-1524.2018.07.02

• Animal Science • Previous Articles Next Articles

Effects of DON and ZEA on IL-2, IL-4, IL-10 and IFN-γ of chicken spleen lymphocytes cultured in vitro

ZHANG Zhuo¹, GU Yanfei¹, YANG Shasha¹, WANG Yachao², DENG Junliang¹, REN Zhihua^{1, *}

1.College of Veterinary Medicine, Sichuan Agricultural University, Chengdu 611130, China;
2. Southwest University of Science and Technology, Mianyang 621010, China

Received:2017-09-21 Online:2018-07-20 Published:2018-08-02

Abstract

Abstract: The purpose of this study was to investigate the effects of DON and ZEA on the secretion of IL-2, IL-4, IL-10 and IFN-γ in cultured chicken spleen lymphocytes in vitro. The exposure doses of DON and ZEA were DON 0.012 5 μg·mL^-1 and ZEA 0.006 25 μg·mL^-1, DON 0.05 μg·mL^-1 and ZEA 0.025 μg·mL^-1, DON 0.2 μg·mL^-1 and ZEA 0.1 μg·mL^-1, DON 0.8 μg·mL^-1 and ZEA 0.4 μg·mL^-1, individually. Then, the effects of different doses of DON and ZEA on the concentrations and mRNA expressions of IL-2, IL-4, IL-10, and IFN-γ of chicken splenic lymphocytes in vitro were investigated. The results were as followed: The concentrations of IL-10 and IFN-γ, and the mRNA expressions of IL-2, IL-4, and IL-10 were increased with the DON and ZEA concentrations increasing (P<0.01). However, the concentrations of IL-2 and IL-4 were decreased with the DON and ZEA concentrations increasing (P<0.01). And the mRNA expressions of IFN-γ were increased at first and then decreased (P<0.01). DON combined with ZEA can affect the secretion of the IL-2, IL-4, IL-10 and IFN-γ with a dose-dependent. DON, ZEA combined with the spleen lymphocytes of the infected chicken to increase or decrease the level of inflammatory cytokines. The imbalance of Th1 and Th2 cytokines can result in cellular immune damage, which can be toxic to cells.

Key words: DON, ZEA, combined exposure, splenic lymphocyte, cytokines

CLC Number:

S859.87

ZHANG Zhuo, GU Yanfei, YANG Shasha, WANG Yachao, DENG Junliang, REN Zhihua. Effects of DON and ZEA on IL-2, IL-4, IL-10 and IFN-γ of chicken spleen lymphocytes cultured in vitro[J]. , 2018, 30(7): 1110-1115.

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Effects of DON and ZEA on IL-2, IL-4, IL-10 and IFN-γ of chicken spleen lymphocytes cultured in vitro

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