Effects of spermidine on immune organ index and expression of inflammatory cytokines in mice

doi:10.3969/j.issn.1004-1524.2017.02.04

›› 2017, Vol. 29 ›› Issue (2): 200-205.DOI: 10.3969/j.issn.1004-1524.2017.02.04

• Animal Science • Previous Articles Next Articles

Effects of spermidine on immune organ index and expression of inflammatory cytokines in mice

XU Qilin, KANG Bo^*, JIANG Dongmei^*, WEI Chunye, JIA Yilin, YI Zhixin, CHEN Ziyu

College of Animal Science and Technology, Sichuan Agricultural University/Farm Animal Genetic Resources Exploration and Innovation Key Laboratory of Sichuan Province, Chengdu 611130, China

Received:2016-08-22 Online:2017-02-15 Published:2017-03-06

Abstract

Abstract: To clarify the effects of exogenous spermidine on mice immune organs index and expression of inflammatory cytokines in thymus and spleen, 0, 0.05, 0.10 and 0.15 mg·g^-1 (body weight) spermidine were intraperitoneal injected for the six-week Kunming mice. The thymus and spleen index were measured after 24 h and the expression of TNF-α、IL-1β、IL-6 and IFN-γ in thymus and spleen were assessed by quantitative real-time PCR. The results showed that, compared with the control group, intraperitoneal injection of 0.15 mg·g^-1 spermidine group significantly (P<0.05) decreased the thymus index and the spleen index. 0.1 mg·g^-1 spermidine group enhanced the thymus IFN-γ expression (P<0.05). In the spleen, the expressions of TNF-α and IL-1β in 0.05 mg·g^-1 spermidine group were significantly (P<0.05) lower than those of the control group, but the expressions of the IL-6 and IFN-γ gene in 0.1 mg·g^-1 spermidine group were significantly higher than those of the control group (P<0.05). The expression of TNF-α in 0.15 mg·g^-1 spermidine group was significantly (P<0.05) lower than that of the control group. Thus, the spermidine showed tissue specific and dose dependent manner on the expression of inflammatory cytokines in mice. Low concentration of spermidine had anti-inflammatory effect by inhibiting the expression of inflammatory cytokines, but high-dose spermidine could also induce inflammation.

Key words: spermidine, thymus index, spleen index, inflammatory cytokines

CLC Number:

XU Qilin, KANG Bo, JIANG Dongmei, WEI Chunye, JIA Yilin, YI Zhixin, CHEN Ziyu. Effects of spermidine on immune organ index and expression of inflammatory cytokines in mice[J]. , 2017, 29(2): 200-205.

References

[1] MINOIS N, CARMONA-GUTIERREZ D, MADEO F. Polyamines in aging and disease [J]. Aging (Albany NY), 2011, 3(8):716-732.
[2] MILLER-FLEMING L, OLIN-SANDOVAL V, CAMPBELL K, et al. Remaining mysteries of molecular biology: the role of polyamines in the cell [J]. Journal of Molecular Biology, 2015, 427(21):3389-3406.
[3] BJELAKOVIĆ G, STOJANOVIĆ I, STOIMENOV T J, et al. Metabolic correlations of glucocorticoids and polyamines in inflammation and apoptosis [J]. Amino Acids, 2010, 39(1):29-43.
[4] 樊桂成, 荣新洲, 王学敏, 等. 外源性腐胺和尸胺对兔外周血炎症因子的影响 [J]. 中华烧伤杂志,2012,28(6):451-454.
FAN G C, RONG X Z, WANG X M, et al. Influence of exogenous putrescine and cadaverine on pro-inflammatory factors in the peripheral blood of rabbits [J].Chinese Journal of Burns, 2012, 28(6):451-454. (in Chinese with English abstract)
[5] SOULET D, RIVEST S. Polyamines play a critical role in the control of the innate immune response in the mouse central nervous system [J]. The Journal of Cell Biology, 2003, 162(2):257-268.
[6] TER STEEGE J C, FORGET P P, BUURMAN W A. Oral spermine administration inhibits nitric oxide-mediated intestinal damage and levels of systemic inflammatory mediators in a mouse endotoxin model [J]. Shock, 1999, 11(2):115-119.
[7] ZHANG M, CARAGINE T, WANG H, et al. Spermine inhibits proinflammatory cytokine synthesis in human mononuclear cells: a counterregulatory mechanism that restrains the immune response [J]. The Journal of Experimental Medicine, 1997, 185(10):1759-1768.
[8] HASKȮ G, KUHEL D G, MARTON A, et al. Spermine differentially regulates the production of interleukin-12 p40 and interleukin-10 and suppresses the release of the T helper 1 cytokine interferon-gamma[J]. Shock, 2000, 14(2):144-149.
[9] 张少帅, 甄龙, 冯京海, 等. 持续偏热处理对肉仔鸡免疫器官指数、小肠形态结构和黏膜免疫指标的影响 [J]. 动物营养学报,2015,27(12):3887-3894.
ZHANG S S, ZHEN L, FENG J H, et al. Effect of continuous partial heart treatment on immune organ indexes, small intestinal morphology and mucosal immunity indexes of broiler chickens [J]. Chinese Journal of Animal Nutrition, 2015, 27(12):3887-3894. (in Chinese with English abstract)
[10] 周岳平,肖能坎,荣新洲,等. 外源性腐胺对正常大鼠肾功能和细胞凋亡的影响[J]. 南方医科大学学报,2012,32(11):1651-1654.
ZHOU Y P, XIAO N K, RONG X Z, et al. Exogenous putrescine causes renal function impairment and cell apoptosis in rats [J]. Journal of Southern Medical University, 2012, 32(11):1651-1654. (in Chinese with English abstract)
[11] 郑灿财,黄艳玲,肖芳. 铬源与铬水平对常规饲养肉仔鸡生长性能、免疫器官指数及肉品质的影响 [J]. 动物营养学报,2015,27(8):2378-2387.
ZHENG C C, HUANG Y L, XIAO F. Effect of chromium sources and chromium levels on growth performance, immune organ indexes and meat quality of broilers under normal rearing condition [J]. Chinese Journal of Animal Nutrition, 2015, 27(8):2378-2387. (in Chinese with English abstract)
[12] 赵宏涛,何余湧,陆伟. 外源精胺对断奶仔猪血液精胺含量、脏器发育和生产性能的影响 [J]. 饲料工业,2015,36(11):15-17.
ZHAO H T, HE Y Y, LU W. Effect of exogenous spermine on the level of spermine on blood, organ development and performance of weaner piglets [J]. Feed Industry, 2015, 36(11):15-17. (in Chinese with English abstract)
[13] THOMAS T, THOMAS T J. Polyamines in cell growth and cell death: molecular mechanisms and therapeutic applications [J]. Cellular and Molecular Life Sciences, 2001, 58(2):244-258.
[14] WOOD P L, KHAN M A, MOSKAL J R. The concept of “aldehyde load” in neurodegenerative mechanisms: cytotoxicity of the polyamine degradation products hydrogen peroxide, acrolein, 3-aminopropanal, 3-acetamidopropanal and 4-aminobutanal in a retinal ganglion cell line [J]. Brain Research, 2007, 1145:150-156.
[15] KUANG S Y, XIAO W W, FENG L, et al. Effects of graded levels of dietary methionine hydroxy analogue on immune response and antioxidant status of immune organs in juvenile Jian carp (Cyprinus carpio var. Jian) [J]. Fish & Shellfish Immunology, 2012, 32(5):629-636.
[16] WEI C, WANG Y, LI M, et al. Spermine inhibits endoplasmic reticulum stress-induced apoptosis: a new strategy to prevent cardiomyocyte apoptosis [J]. Cellular Physiology and Biochemistry, 2016, 38(2):531-544.
[17] KUMURA S, TERATANI T, FUJIMOTO Y, et al. Oral administration of polyamines ameliorates liver ischemia/reperfusion injury and promotes liver regeneration in rats [J]. Liver Transplantation, 2016, 22(9):1231-1244.
[18] 刘玲,赵弘卿,王昕华,等. 重症肺炎患者干扰素-γ, IL-6和TNF-α含量变化研究 [J]. 现代生物医学进展,2015,15(36):7107-7110.
LIU L, ZHAO H Q, WANG X H, et al. Study on the changes of interferon-γ, IL-6 and TNF-α of patients with severe pneumonia [J]. Progress in Modern Biomedine, 2015, 15(36):7107-7110. (in Chinese with English abstract)
[19] YIM C Y, BASTIAN N R, SMITH J C, et al. Macrophage nitric oxide synthesis delays progression of ultraviolet light-induced murine skin cancers [J]. Cancer Research, 1993, 53(22):5507-5511.
[20] PAUL S, KANG S C. Natural polyamine inhibits mouse skin inflammation and macrophage activation [J]. Inflammation Research, 2013, 62(7):681-688.
[21] THAKUR P, NEHRU B. Anti-inflammatory properties rather than anti-oxidant capability is the major mechanism of neuroprotection by sodium salicylate in a chronic rotenone model of Parkinson's disease [J]. Neuroscience, 2013, 231:420-431.
[22] YE L, HUANG Y, ZHAO L, et al. IL-1β and TNF-α induce neurotoxicity through glutamate production: a potential role for neuronal glutaminase [J]. Journal of Neurochemistry, 2013, 125(6):897-908.
[23] 殷银霞,许雅清,李海龙,等. IL-1、IL-6、TNF-α及 IFN-γ在脾肾阳虚型溃疡性结肠炎模型大鼠血清及组织中的表达 [J]. 中国实验动物学报, 2015, 23(2):139-142.
YIN Y X, XU Y Q, LI H L, et al. Expression and significance of IL-1, IL-6, TNF-α and INF-γ in serum and colon tissue in the rat models of ulcerative colitis with spleen and kidney yang deficiency [J]. Acta Laboratorium Animallis Scientia Sinica, 2015, 23(2):139-142. (in Chinese with English abstract)
[24] CHOI Y H, PARK H Y. Anti-inflammatory effects of spermidine in lipopolysaccharide-stimulated BV2 microglial cells [J]. Journal of Biomedical Science, 2012, 19(1):31.
[25] MERENTIE M, UIMARI A, PIETIL M, et al. Oxidative stress and inflammation in the pathogenesis of activated polyamine catabolism-induced acute pancreatitis [J]. Amino Acids, 2007, 33(2):323-330.
[26] JAMWAL S,KUMAR P. Spermidine ameliorates 3-nitropropionic acid (3-NP)-induced striatal toxicity: possible role of oxidative stress, neuroinflammation, and neurotransmitters [J]. Physiology & Behavior, 2016, 155:180-187.
[27] JAMWAL S, SINGH S, KAUR N, et al. Protective effect of spermidine against excitotoxic neuronal death induced by quinolinic acid in rats: possible neurotransmitters and neuroinflammatory mechanism [J]. Neurotoxicity Research, 2015, 28(2):171-184.
[28] SZABO C, SOUTHAN G J, WOOD E, et al. Inhibition by spermine of the induction of nitric oxide synthase in J774. 2 macrophages: requirement of a serum factor [J]. British Journal of Pharmacology, 1994, 112(2):355-356.

Effects of spermidine on immune organ index and expression of inflammatory cytokines in mice

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