›› 2017, Vol. 29 ›› Issue (2): 193-199.DOI: 10.3969/j.issn.1004-1524.2017.02.03

• Animal Science • Previous Articles     Next Articles

Isolation, identification and phylogenetic analysis of Lactobacillus from vagina of giant panda

MA Xiaoping1, YANG Tianyi1, YU Yan1, ZHANG Zhihe2, *, WANG Chengdong3, GU Yu4, *   

  1. 1. College of Veterinary Medicine, Sichuan Agricultural University/ Key Laboratory of Animal Disease and Human Health of Sichuan Province, Chengdu 611130, China;
    2. Chengdu Research Base of Giant Panda Breeding, Chengdu 610000, China;
    3. China Conservation and Research Center for the Giant Panda, Ya'an 625000, China;;
    4. College of Life Science, Sichuan Agricultural University, Ya'an 625014, China;
  • Received:2016-07-19 Online:2017-02-15 Published:2017-03-06

Abstract: In order to separate and identify the Lactobacillus from vagina of giant panda, the vaginal secretions were taken from estrus female giant panda and women. The isolated Lactobacillus strains were identified by morphology, biochemical characteristics, 16S rDNA sequencing and phylogenetic analysis. The results showed that a total of 6 gram-positive Lactobacillus strains were obtained. Lactobacillus salivarius GPV01, Lactobacillus plantarum GPV02, Lactobacillus saerimneri GPV03 were isolated from the vaginal secretion of the giant panda (test group), and Lactobacillus acidophilus WAV01, Lactobacillus crispatus WAV02, Lactobacillus plantarum WAV03 were isolated from the vaginal secretion of the woman (control group). It was interesting that the giant panda and woman all had L. plantarum in the vagina (GPV02 and WAV03). This result would lay the foundation for studying biological property of vagina Lactobacillus of giant panda and exploiting vaginal probiotics for giant panda.

Key words: giant panda, vagina, Lactobacillus, phylogenetics

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