Effect of Wuling San on copper poisoning in experimental rats

doi:10.3969/j.issn.1004-1524.2017.02.05

›› 2017, Vol. 29 ›› Issue (2): 206-212.DOI: 10.3969/j.issn.1004-1524.2017.02.05

• Animal Science • Previous Articles Next Articles

Effect of Wuling San on copper poisoning in experimental rats

CHENG Yan¹, LIU Yayun¹, ZHANG Fangjie¹, HUANG Cuihua¹, KANG Youxi¹, HAN Chunyang¹, CHEN Dingding^{2, *}, LIU Cuiyan^{1, *}

1. College of Animal Science and Technology, Anhui Agricultural University, Hefei 230036, China;
2. Biotechnology Center, Anhui Agricultural University, Hefei 230036, China

Received:2016-09-09 Online:2017-02-15 Published:2017-03-06

Abstract

Abstract: In order to investigate the effects of Wuling San, a Chinese herbal medicine compound powder with promoting diuresis, on the copper excretion in animal, the pathological model of subchronic copper poisoning in SD rats was established by feeding high copper diet, and then Wuling San as a therapeutic drug was given by means of intragastric administration to rats. After the success of building models and at the end of treatment, the blood sample of rats was collected by cardiac puncture for determining copper content with the method of flame atomic absorption spectrometry, and the activities of serum AST, LDH and SOD were detected by reagent kit. At the end of the experiment, all the rats were anatomized to observe the changes of pathologic autopsy of the internal organs, and the histopathologic changes of liver and kidney. Results showed that compared with control group, the rats of the model group were slow in velocity of body weight increasement, the obvious pathological changes of liver and kidney structure were found and the level of blood copper was significantly higher (P<0.01), indicating that the model of subchronic copper poisoning in rats was successfully established. After treated with Wuling San, compared with model group, the treatment group had less detectable changes in histopathology, the level of blood copper was significantly lower (P<0.05), SOD activity increased (P<0.05) and AST and LDH activities declined (P<0.05). The above five indexes had no significant differences between the treatment group and the control group (P<0.05). The results of this study indicated that Wuling San could effectively promote the excretion of copper in rats, alleviate the damage of liver and kidney, so it could be used to treat clinical subchronic copper poisoning of animals.

Key words: Wuling San, rat, copper poisoning, therapy

CLC Number:

S853.91

CHENG Yan, LIU Yayun, ZHANG Fangjie, HUANG Cuihua, KANG Youxi, HAN Chunyang, CHEN Dingding, LIU Cuiyan. Effect of Wuling San on copper poisoning in experimental rats[J]. , 2017, 29(2): 206-212.

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Effect of Wuling San on copper poisoning in experimental rats

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