Preliminary study on transcriptional regulation sequence of chicken NLRC5 promoter

doi:10.3969/j.issn.1004-1524.2017.03.07

›› 2017, Vol. 29 ›› Issue (3): 395-400.DOI: 10.3969/j.issn.1004-1524.2017.03.07

• Animal Science • Previous Articles Next Articles

Preliminary study on transcriptional regulation sequence of chicken NLRC5 promoter

ZHANG Xin, GUO Xiaomin, CHANG Guobin^*, ZHU Pengfei, XU Lu, QIU Lingling, ZHANG Yang, XU Qi, CHEN Guohong

College of Animal Science and Technology, Yangzhou University, Yangzhou 225009, China

Received:2016-10-01 Online:2017-03-20 Published:2017-03-31

Abstract

Abstract: In order to explore the potential regulation mechanism of chicken NLRC5 promoter, deletion fragments of chicken NLRC5 promoter was cloned and inserted into pGL3-basic vector to construct recombinant plasmids for transfecting DF1 cells. Dual-luciferase assay was carried out to find the core regulation region. Then target sequence capture assay was used to detect SNPs in the core region of NLRC5 promoter of 27 chicken breeds. Finally, transcription factor binding sites and CpG islands were predicted by using online softwares TRANSFAC, JASPAR and MethPrimer. The results showed that NLRC5 promoter had 2 core regions, 1 to 617 and 1 448 to 2 108, respectively. There were 3 SNPs loci totally in the first core region in all breeds. SNP1 was found to influence the transcription factor Hic1 binding site. However, SNPs in the first core region did not affect CpG island. The results above suggested that NLRC5 promoter activity might be regulated by different factors, but SNPs didn’t influence the promoter activity at methylation level.

Key words: chicken, NLRC5 promoter, SNPs, transcription factor binding site, CpG island

CLC Number:

S852.4

ZHANG Xin, GUO Xiaomin, CHANG Guobin, ZHU Pengfei, XU Lu, QIU Lingling, ZHANG Yang, XU Qi, CHEN Guohong. Preliminary study on transcriptional regulation sequence of chicken NLRC5 promoter[J]. , 2017, 29(3): 395-400.

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Preliminary study on transcriptional regulation sequence of chicken NLRC5 promoter

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